akhoza centrifugation kulekanitsa nanoparticles zosungunuka

Chiyambi

Kutsegula centrifugation ndi njira yomwe imagwiritsidwa ntchito kwambiri m'munda wa nanotechnology kulekanitsa tinthu tating'ono ...

Kumvetsetsa Centrifugation

Kugawanitsa zinthu ndi njira yomwe imagwiritsa ntchito mphamvu ya centrifugal kulekanitsa tinthu tating'onoting'ono tosiyanasiyana. Zimaphatikizapo kupotoza chitsanzo pa liwiro lalikulu, zomwe zimapangitsa tinthu tating'onoting'ono kukhala tochepa kutengera kulemera kwawo. Mfundo yaikulu ya kugawanitsa zinthu ndi yakuti tinthu tating'onoting'ono tomwe timakhala tolimba tidzakhazikika mofulumira ndikupanga pellet pansi pa chubu, pomwe tinthu tating'onoting'ono topepuka tidzatsala mu supernatant.

1. Njira Zachikhalidwe Zogwiritsira Ntchito Centrifugation

Pankhani yolekanitsa tinthu tating'onoting'ono tosungunuka, njira zachikhalidwe zoyezera mlengalenga sizingapereke zotsatira zabwino. Popeza tinthu tating'onoting'ono tosungunuka timakonda kusungunuka kapena kufalikira mumadzimadzi, zingakhale zovuta kuyika matope bwino. Komabe, kusintha kwina kwa njira zachikhalidwe zoyezera mlengalenga kwapangidwa kuti kuthetse vutoli.

2. Kuchepetsa Mphamvu ya Maginito

Kuchuluka kwa ma gradient centrifugation ndi njira yomwe imagwiritsidwa ntchito kwambiri kulekanitsa tinthu tomwe tili ndi ma densities ofanana. Pankhani ya tinthu tating'onoting'ono tomwe timasungunuka, density medium ingasinthidwe kuti igwirizane ndi kuchuluka komwe tinthu tating'onoting'ono timafunikira. Mwa kuyika chitsanzo mosamala pamwamba pa density gradient yopangidwa kale, tinthu tating'onoting'ono tingalekanitsidwe malinga ndi ma densities awo pamene akusunthira ku malo awo ofanana mkati mwa medium.

3. Kujambula kwa Kukula ndi Kuchotsa Kukula (SEC)

Chromatography ya Kukula-Kuchotsa (SEC), yomwe imadziwikanso kuti gel filtration chromatography, ndi njira yotchuka yomwe imagwiritsidwa ntchito kulekanitsa mamolekyu kapena tinthu tating'onoting'ono kutengera kukula kwawo. Pankhani ya tinthu tating'onoting'ono tomwe timasungunuka, SEC ikhoza kusinthidwa kuti ilekanitse tinthu tating'onoting'ono kutengera kukula kwawo kwa hydrodynamic. Tinthu tating'onoting'onoting'onoting'ono timalowa m'mabowo a gawo lokhazikika, zomwe zimapangitsa kuti nthawi yayitali isungidwe, pomwe tinthu tating'onoting'onoting'onoting'onoting'onoting'ono timatha kutuluka mwachangu.

4. Kuchepetsa mphamvu ya ultracentrifugation

Kupondereza kwa Ultracentrifugation ndi njira yapamwamba kwambiri yopondereza yomwe imaphatikizapo liwiro lalikulu komanso nthawi yayitali yogwiritsira ntchito kuposa njira zachikhalidwe. Njirayi ingagwiritsidwe ntchito kulekanitsa tinthu tating'onoting'ono tosungunuka mwa kusintha mphamvu ya centrifugal yomwe imagwiritsidwa ntchito ndikuwonjezera nthawi yogwiritsira ntchito. Mwa kupeza mikhalidwe yabwino kwambiri, tinthu tating'onoting'ono tosungunuka titha kulekanitsidwa bwino kutengera ma coefficients awo a sedimentation, zomwe zimathandiza kuti tisonkhanitse bwino tinthu tating'onoting'ono tomwe tikufuna.

5. Kuchulukana kwa Maginito a Ultracentrifugation

Kuchuluka kwa ma gradient ultracentrifugation kumaphatikiza mfundo za density gradient centrifugation ndi ultracentrifugation kuti zilekanitse nanoparticles zosungunuka. Kuchuluka kwa ma density gradient kumakhazikitsidwa pogwiritsa ntchito medium yoyenera, ndipo chitsanzocho chimayikidwa pamwamba. Kuchuluka kwa ma centrifugation kumachitika kuti zilekanitse nanoparticles kutengera kuchuluka kwawo ndikupeza kugawa kwakukulu. Njirayi imalola kulekanitsa molondola kukula kapena mitundu yosiyanasiyana ya nanoparticles mu mawonekedwe osungunuka.

Mapeto

Njira zoyezera centrifugation zathandiza kwambiri pakulekanitsa tinthu tating'ono ...