tuaj yeem centrifugation cais cov nanoparticles uas yaj tau
Kev Taw Qhia
Centrifugation yog ib txoj kev siv dav hauv thaj chaw ntawm nanotechnology los cais ntau yam nanoparticles raws li lawv cov khoom, xws li qhov loj me, cov duab, thiab qhov ceev. Cov nanoparticles yaj tau, tshwj xeeb, ua rau muaj kev cov nyom tshwj xeeb vim lawv muaj peev xwm yaj lossis tawg hauv cov kua nruab nrab. Tsab xov xwm no nkag mus rau hauv daim ntawv thov ntawm centrifugation hauv kev sib cais cov nanoparticles yaj tau thiab tshawb nrhiav cov tswv yim sib txawv siv los ua kom muaj kev sib cais zoo.
Nkag Siab Txog Kev Siv Centrifugation
Kev centrifugation yog ib qho txheej txheem uas siv lub zog centrifugal los cais cov khoom me me hauv cov khoom sib xyaw ua ke. Nws cuam tshuam nrog kev tig cov qauv ntawm qhov ceev ceev, uas ua rau cov khoom me me ua cov av raws li lawv qhov hnyav. Lub hauv paus ntsiab lus tom qab centrifugation yog tias cov khoom me me yuav poob sai dua thiab tsim cov pellet hauv qab ntawm lub raj, thaum cov khoom me me dua yuav nyob hauv cov supernatant.
1. Cov Txuj Ci Centrifugation Ib Txwm Muaj
Hauv cov ntsiab lus ntawm kev sib cais cov nanoparticles uas yaj tau, cov txheej txheem centrifugation ib txwm muaj tej zaum yuav tsis ua rau muaj txiaj ntsig zoo. Vim tias cov nanoparticles uas yaj tau feem ntau yaj lossis tawg hauv cov kua nruab nrab, lawv tuaj yeem nyuaj rau cov av noo kom zoo. Txawm li cas los xij, ntau qhov kev hloov kho rau cov txheej txheem centrifugation ib txwm muaj tau tsim los daws qhov teeb meem no.
2. Kev Siv Centrifugation Gradient Ceev
Kev siv centrifugation uas muaj qhov ceev sib txawv yog ib txoj kev siv dav dav los cais cov khoom me me uas muaj qhov ceev sib xws. Hauv cov khoom me me uas yaj tau, qhov ceev nruab nrab tuaj yeem hloov kho kom phim qhov ceev ntawm cov khoom me me uas xav tau. Los ntawm kev ua tib zoo muab cov qauv tso rau saum qhov ceev gradient uas tau tsim ua ntej, cov nanoparticles tuaj yeem sib cais raws li lawv qhov ceev thaum lawv mus rau lawv qhov chaw sib npaug hauv qhov nruab nrab.
3. Kev Siv Chromatography Los Ntawm Qhov Loj (SEC)
Kev Siv Chromatography Loj-Tsis suav nrog (SEC), tseem hu ua gel filtration chromatography, yog ib txoj kev nrov siv los cais cov molecules lossis cov khoom me me raws li lawv qhov loj me. Hauv cov ntaub ntawv ntawm cov nanoparticles uas yaj tau, SEC tuaj yeem hloov kho kom zoo cais cov nanoparticles raws li lawv qhov loj me hydrodynamic. Cov nanoparticles me dua yuav nkag mus rau hauv cov qhov ntawm theem ruaj khov, ua rau lub sijhawm khaws cia ntev dua, thaum cov nanoparticles loj dua yuav tawm sai dua.
4. Kev siv tshuab centrifugation ntau heev
Ultracentrifugation yog ib hom kev centrifugation siab heev uas cuam tshuam nrog kev ceev dua thiab lub sijhawm khiav ntev dua li cov txheej txheem ib txwm muaj. Cov txheej txheem no tuaj yeem siv los cais cov nanoparticles uas yaj tau los ntawm kev kho lub zog centrifugal siv thiab ua kom lub sijhawm khiav zoo dua. Los ntawm kev nrhiav cov xwm txheej zoo tshaj plaws, cov nanoparticles uas yaj tau tuaj yeem sib cais tau zoo raws li lawv cov coefficients sedimentation, uas tso cai rau kev sau cov nanoparticles uas tau tsom mus rau.
5. Kev Siv Lub Tshuab Centrifugation Ceev-Gradient
Kev siv tshuab density-gradient ultracentrifugation los cais cov nanoparticles uas yaj tau. Kev siv tshuab density gradient los tsim ib qho density gradient los ntawm kev siv ib qho chaw nruab nrab uas tsim nyog, thiab cov qauv raug muab tso rau saum toj. Tom qab ntawd, kev siv tshuab density gradient los cais cov nanoparticles raws li lawv qhov ceev thiab ua tiav kev faib ua feem siab. Cov txheej txheem no tso cai rau kev sib cais meej ntawm ntau qhov ntau thiab tsawg lossis hom nanoparticles hauv daim ntawv yaj tau.
Xaus lus
Cov txheej txheem centrifugation tau ua pov thawj tias muaj txiaj ntsig zoo hauv kev sib cais ntawm ntau yam nanoparticles, suav nrog cov nanoparticles uas yaj tau. Los ntawm kev hloov kho cov txheej txheem centrifugation ib txwm muaj, cov kws tshawb fawb tau kov yeej cov teeb meem cuam tshuam nrog kev yaj ntawm nanoparticles thiab ua tiav kev sib cais zoo. Density gradient centrifugation, size-exclusion chromatography, ultracentrifugation, thiab density-gradient ultracentrifugation yog cov tswv yim sib txawv siv los cais cov nanoparticles uas yaj tau zoo. Cov txheej txheem no ua rau muaj kev nce qib ntxiv thiab kev siv nanotechnology hauv ntau qhov chaw xws li tshuaj mus rau kev tshawb fawb ib puag ncig.
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