ike centrifugation kewapụrụ nanoparticles soluble

Okwu Mmalite

Centrifugation bụ usoro a na-ejikarị eme ihe n'ọhịa nanotechnology iji kewaa ọtụtụ nanoparticles dabere na njirimara ha, dịka nha, ọdịdị, na njupụta. Nanoparticles ndị na-agbaze agbaze, karịsịa, na-eweta ihe ịma aka pụrụ iche n'ihi ikike ha nwere ịgbaze ma ọ bụ gbasaa na mmiri mmiri. Isiokwu a na-eleba anya n'itinye centrifugation n'ime nkewa nke nanoparticles ndị na-agbaze agbaze ma na-enyocha atụmatụ dị iche iche eji enweta nkewa dị irè.

Ịghọta Centrifugation

Ịsa mbara igwe bụ usoro nke na-eji ike centrifugal kewaa ihe ndị dị n'ime ngwakọta dị iche iche. Ọ gụnyere ịgbagharị ihe nlele n'ọsọ dị elu, nke na-eme ka ihe ndị dị n'ime ala dabere na oke ha. Ụkpụrụ dị n'azụ centrifugation bụ na ihe ndị dị n'ime ya ga-adaba ngwa ngwa ma mepụta pellet n'ala nke tube ahụ, ebe ihe ndị dị mfe ga-anọgide na supernatant.

1. Usoro nhazi centrifugation ọdịnala

N'ihe gbasara ikewapụ nanoparticles ndị na-agbaze agbaze, usoro centrifugation ọdịnala nwere ike ọ gaghị eweta nsonaazụ na-eju afọ. Ebe ọ bụ na nanoparticles ndị na-agbaze agbaze na-agbaze ma ọ bụ gbasaa na mmiri mmiri, ha nwere ike isiri ike ime ka mmiri ghara ịdị nke ọma. Agbanyeghị, emepụtala ọtụtụ mgbanwe na usoro centrifugation ọdịnala iji dozie okwu a.

2. Ntinye njupụta nke Gradient

Ngụkọta njupụta nke ihe ndị dị n'ime ihe ndị a bụ usoro e ji ekewapụ ihe ndị nwere njupụta yiri nke ahụ. N'ihe gbasara ihe ndị na-agbaze agbaze, enwere ike ịgbanwe ihe ndị dị n'ime ihe ndị a ka ha kwekọọ njupụta achọrọ nke ihe ndị a chọrọ. Site n'iji nlezianya tinye ihe nlele ahụ n'elu ihe ndị e ji njupụta mee, enwere ike ikewapụ ihe ndị dị n'ime ihe ndị a dịka njupụta ha si dị ka ha si dị ka ha si aga n'ọnọdụ nhazi ha n'ime ihe ndị a.

3. Chromatography nke Mwepụ Nha (SEC)

Chromatography nke nha-mwepu (SEC), nke a makwaara dị ka gel filtration chromatography, bụ usoro a ma ama nke eji kewaa molekul ma ọ bụ ihe ndị dị n'ime ya dabere na nha ha. N'ihe gbasara nanoparticles na-agbaze agbaze, enwere ike ịgbanwe SEC ka ọ kewaa nanoparticles nke ọma dabere na nha hydrodynamic ha. Nanoparticles ndị pere mpe ga-abanye n'ime oghere nke usoro ahụ na-adịgide adịgide, na-eduga n'oge nchekwa dị ogologo, ebe nanoparticles ndị buru ibu ga-agbapụ ngwa ngwa.

4. Ultracentrifugation

Ultracentrifugation bụ ụdị centrifugation dị elu nke gụnyere ọsọ dị elu na oge ọsọ dị ogologo karịa ụzọ ọdịnala. Enwere ike iji usoro a kewaa nanoparticles na-agbaze site na ịgbanwe ike centrifugal etinyere na ime ka oge ọsọ dị mma. Site n'ịchọta ọnọdụ kacha mma, enwere ike kewaa nanoparticles na-agbaze nke ọma dabere na ihe ndị na-eme ka mmiri ghara ịdị, na-enye ohere maka nchịkọta nke nanoparticles ezubere iche nke ọma.

5. Njupụta-Gradient Ultracentrifugation

Njupụta na-ejikọta ụkpụrụ nke njupụta na-agbanwe agbanwe na ultracentrifugation iji kewaa nanoparticles ndị na-agbaze agbaze. A na-eji usoro kwesịrị ekwesị guzobe njupụta, a na-etinyekwa ihe nlele ahụ n'elu. A na-eme Ultracentrifugation iji kewaa nanoparticles dabere na njupụta ha ma nweta nkewa dị elu. Usoro a na-enye ohere maka nkewa ziri ezi nke nha ma ọ bụ ụdị nanoparticles dị iche iche n'ụdị na-agbaze agbaze.

Mmechi

Usoro nhazi centrifugation egosila na ọ bara uru nke ukwuu n'ikewapụ ọtụtụ nanoparticles dị iche iche, gụnyere nanoparticles na-agbaze agbaze. Site n'ịgbanwe ụzọ nhazi centrifugation ọdịnala, ndị nchọpụta enwewo ike imeri ihe ịma aka ndị metụtara ịgbaze nanoparticles ma nweta nkewa dị irè. centrifugation gradient density, chromatography nke mwepụ nha, ultracentrifugation, na njupụta-gradient ultracentrifugation so n'ime atụmatụ dị iche iche eji kewaa nanoparticles na-agbaze agbaze nke ọma. Usoro ndị a na-emeghe ụzọ maka ọganihu na ojiji nke nanotechnology n'ọtụtụ ngalaba site na ọgwụ ruo na sayensị gburugburu ebe obibi.