centrifugation-ku wuxuu kala saari karaa nanoparticles milmi kara
Hordhac
Centrifugation waa farsamo si weyn loo isticmaalo oo ku jirta cilmiga nanotechnology si loo kala saaro nanoparticles kala duwan iyadoo lagu saleynayo sifooyinkooda, sida cabbirka, qaabka, iyo cufnaanta. Nanoparticles-ka milma, gaar ahaan, waxay keenaan caqabad gaar ah sababtoo ah awooddooda ay ku milmi karaan ama ku kala firdhi karaan meel dareere ah. Maqaalkani wuxuu si qoto dheer u eegayaa isticmaalka centrifugation-ka ee kala-soocidda nanoparticles-ka milma wuxuuna sahaminayaa xeeladaha kala duwan ee loo isticmaalo in lagu gaaro kala-soocid hufan.
Fahmidda Centrifugation-ka
Centrifugation waa hab loo adeegsado xoogga centrifugal si loo kala saaro walxaha isku dhafan oo kala duwan. Waxay ku lug leedahay wareejinta muunad xawaare sare leh, taasoo keenta in walxaha ay ku dhacaan wasakh iyadoo lagu saleynayo cufnaantooda. Mabda'a ka dambeeya centrifugation-ka ayaa ah in walxaha cufan ay si dhakhso ah u degi doonaan oo ay ka samaysan doonaan pellet salka tuubada, halka walxaha khafiifka ah ay ku sii jiri doonaan dusha sare.
1. Farsamooyinka Centrifugation-ka Dhaqanka
Marka la eego kala soocidda nanoparticles-ka milmi kara, farsamooyinka centrifugation-ka dhaqameed waxaa laga yaabaa inaysan soo saarin natiijooyin ku filan. Maadaama nanoparticles-ka milmi kara ay u muuqdaan inay ku milmaan ama ku kala firdhaan dareeraha, waxay noqon karaan kuwo adag in si wax ku ool ah loo fadhiisiyo. Si kastaba ha ahaatee, dhowr wax ka beddel oo lagu sameeyay hababka centrifugation-ka caadiga ah ayaa la sameeyay si wax looga qabto arrintan.
2. Santuuqaynta Cufnaanta ee Cufnaanta
Centrifugation-ka cufnaanta waa farsamo si weyn loo isticmaalo in lagu kala saaro walxaha leh cufnaanta isku midka ah. Haddii ay jiraan nanoparticles milmi kara, dhexdhexaadka cufnaanta waxaa la hagaajin karaa si uu ula jaanqaado cufnaanta la rabo ee walxaha bartilmaameedka ah. Iyadoo si taxaddar leh loogu dahaadhay muunadda cufnaanta hore loo sameeyay, nanoparticles-ka waxaa loo kala saari karaa iyadoo loo eegayo cufnaantooda marka ay u guuraan boosaskooda dheelitirka ee gudaha dhexdhexaadka.
3. Kareemaynta Cabbirka-Ka-saarista (SEC)
Chromatography-ga Cabbirka-Ka-saarista (SEC), oo sidoo kale loo yaqaan jel filterography chromatography, waa hab caan ah oo loo isticmaalo in lagu kala saaro molecules ama walxaha iyadoo lagu saleynayo cabbirkooda. Xaaladda nanoparticles-ka milma, SEC waxaa loo habeyn karaa si wax ku ool ah u kala sooca nanoparticles iyadoo lagu saleynayo cabbirkooda hydrodynamic. Nanoparticles-ka yaryar waxay geli doonaan daloolada wejiga taagan, taasoo horseedaysa waqti dheer oo hayn ah, halka nanoparticles-ka waaweyn ay si dhakhso ah u bixi doonaan.
4. Ultracentrifugation
Ultracentrifugation waa nooc horumarsan oo centrifugation ah kaas oo ku lug leh xawaare sare iyo waqtiyo socod dheer marka loo eego hababka caadiga ah. Farsamadan waxaa loo isticmaali karaa in lagu kala saaro nanoparticles-ka milmi kara iyadoo la hagaajinayo xoogga centrifugal-ka ee la adeegsaday iyo hagaajinta waqtiga socodka. Iyadoo la helayo xaaladaha ugu habboon, nanoparticles-ka milmi kara si wax ku ool ah ayaa loo kala saari karaa iyadoo lagu saleynayo isku-xidhkooda fadhiisinta, taasoo u oggolaanaysa ururinta hufan ee nanoparticles-ka bartilmaameedsan.
5. Cufnaanta-Guduudaynta Ultracentrifugation
Cufnaanta-gradient ultracentrifugation waxay isku daraysaa mabaadi'da centrifugation cufnaanta iyo ultracentrifugation si loo kala saaro nanoparticles milmi kara. Cufnaanta waxaa la sameeyaa iyadoo la isticmaalayo dhexdhexaad ku habboon, muunaddana waxaa lagu dhejiyaa dusha sare. Ultracentrifugation ayaa markaa la sameeyaa si loo kala saaro nanoparticles iyadoo lagu saleynayo cufnaantooda loona gaaro jajabnaan xallin sare leh. Farsamadani waxay u oggolaanaysaa kala soocidda saxda ah ee cabbirrada kala duwan ama noocyada nanoparticles qaab milmi kara.
Gunaanad
Farsamooyinka centrifugation-ka ayaa noqday kuwo qiimo leh marka la kala saarayo nanoparticles-ka kala duwan, oo ay ku jiraan nanoparticles-ka milma. Iyagoo wax ka beddelaya hababka centrifugation-ka dhaqameed, cilmi-baarayaashu waxay awoodeen inay ka gudbaan caqabadaha la xiriira milmida nanoparticles-ka oo ay gaaraan kala-soocid hufan. centrifugation-ka cufnaanta, chromatography-ga ka-saarista cabbirka, ultracentrifugation, iyo ultracentrifugation-ka cufnaanta-gradient ayaa ka mid ah xeeladaha kala duwan ee loo isticmaalo in si wax ku ool ah loogu kala saaro nanoparticles-ka milma. Farsamooyinkani waxay u gogol xaaraan horumarro dheeraad ah iyo codsiyada nanotechnology-ka ee dhinacyo kala duwan oo u dhexeeya daawada ilaa sayniska deegaanka.
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