hiki ke centrifugation kaʻawale nā nanoparticles soluble
Hoʻolauna
ʻO ka centrifugation kahi ʻenehana i hoʻohana nui ʻia ma ke kahua o ka nanotechnology e hoʻokaʻawale i nā nanoparticles like ʻole e pili ana i ko lākou mau waiwai, e like me ka nui, ke ʻano, a me ka nui. ʻO nā nanoparticles soluble, ʻoi aku hoʻi, ke kau nei i kahi pilikia kūikawā ma muli o ko lākou hiki ke hoʻoheheʻe a hoʻopuehu paha i loko o kahi wai. Ke noi nei kēia ʻatikala i ka hoʻohana ʻana o ka centrifugation i ka hoʻokaʻawale ʻana o nā nanoparticles soluble a ke ʻimi nei i nā ʻano hana like ʻole i hoʻohana ʻia e hoʻokō i ka hoʻokaʻawale pono.
Ke Hoʻomaopopo ʻana i ka Centrifugation
ʻO ke centrifugation kahi hana e hoʻohana ana i ka ikaika centrifugal e hoʻokaʻawale i nā ʻāpana i loko o kahi hui heterogeneous. Pili ia i ka milo ʻana i kahi laʻana ma nā wikiwiki kiʻekiʻe, kahi e hoʻoulu ai i nā ʻāpana e waiho ma muli o ko lākou nuipa. ʻO ke kumumanaʻo ma hope o ke centrifugation, ʻo ia ka noho wikiwiki ʻana o nā ʻāpana paʻa a hana i kahi pellet ma lalo o ka ʻōmole, ʻoiai e noho nā ʻāpana māmā i loko o ka supernatant.
1. Nā ʻenehana Centrifugation Kuʻuna
I loko o ke ʻano o ka hoʻokaʻawale ʻana i nā nanoparticles hiki ke hoʻoheheʻe ʻia, ʻaʻole paha e hāʻawi nā ʻano hana centrifugation kuʻuna i nā hopena kūpono. ʻOiai ʻo nā nanoparticles hiki ke hoʻoheheʻe a hoʻopuehu paha i loko o ka wai, hiki iā lākou ke paʻakikī ke hoʻoheheʻe pono. Eia nō naʻe, ua hoʻomohala ʻia kekahi mau hoʻololi i nā ʻano centrifugation kuʻuna e hoʻoponopono i kēia pilikia.
2. Ke Keʻena Hoʻokaʻawale ʻana o ka Gradient Density
ʻO ka centrifugation gradient density kahi ʻenehana i hoʻohana nui ʻia e hoʻokaʻawale i nā ʻāpana me nā densities like. I ke ʻano o nā nanoparticles hiki ke hoʻoheheʻe ʻia, hiki ke hoʻoponopono ʻia ka medium density e kūlike me ka density i makemake ʻia o nā ʻāpana i manaʻo ʻia. Ma ka hoʻopili pono ʻana i ka laʻana ma luna o kahi gradient density i hana mua ʻia, hiki ke hoʻokaʻawale ʻia nā nanoparticles e like me ko lākou densities i ko lākou neʻe ʻana i ko lākou mau kūlana kaulike i loko o ka medium.
3. Chromatography Hoʻokaʻawale Nui (SEC)
ʻO ka Size-Exclusion Chromatography (SEC), i ʻike ʻia hoʻi ʻo gel filtration chromatography, he ʻano hana kaulana ia i hoʻohana ʻia e hoʻokaʻawale i nā molekala a i ʻole nā ʻāpana ma muli o ko lākou nui. I ke ʻano o nā nanoparticles hiki ke hoʻoheheʻe ʻia, hiki ke hoʻololi ʻia ʻo SEC e hoʻokaʻawale pono i nā nanoparticles ma muli o ko lākou nui hydrodynamic. E komo nā nanoparticles liʻiliʻi i loko o nā pores o ka pae paʻa, e alakaʻi ana i ka manawa paʻa lōʻihi, ʻoiai ʻo nā nanoparticles nui e elute wikiwiki.
4. Hoʻokaʻawale ʻana i ka Ultracentrifugation
ʻO ka Ultracentrifugation kahi ʻano centrifugation holomua e pili ana i nā wikiwiki kiʻekiʻe a me nā manawa holo lōʻihi ma mua o nā ʻano hana maʻamau. Hiki ke hoʻohana ʻia kēia ʻenehana e hoʻokaʻawale i nā nanoparticles soluble ma ka hoʻoponopono ʻana i ka ikaika centrifugal i hoʻopili ʻia a me ka hoʻonui ʻana i ka manawa holo. Ma ka loaʻa ʻana o nā kūlana kūpono, hiki ke hoʻokaʻawale pono ʻia nā nanoparticles soluble ma muli o kā lākou mau coefficients sedimentation, e ʻae ana i ka hōʻiliʻili pono ʻana o nā nanoparticles i kuhikuhi ʻia.
5. Ka Ultracentrifugation Density-Gradient
Hoʻohui ka ultracentrifugation density-gradient i nā loina o ka centrifugation gradient density a me ka ultracentrifugation e hoʻokaʻawale i nā nanoparticles hiki ke hoʻoheheʻe ʻia. Hoʻokumu ʻia kahi gradient density me ka hoʻohana ʻana i kahi medium kūpono, a kau ʻia ka laʻana ma luna. A laila hana ʻia ka Ultracentrifugation e hoʻokaʻawale i nā nanoparticles ma muli o ko lākou density a hoʻokō i ka fractionation kiʻekiʻe. ʻAe kēia ʻenehana i ka hoʻokaʻawale pololei ʻana o nā nui like ʻole a i ʻole nā ʻano nanoparticles i kahi ʻano hiki ke hoʻoheheʻe ʻia.
Hopena
Ua hōʻoia ʻia nā ʻano hana centrifugation he mea waiwai nui i ka hoʻokaʻawale ʻana i nā nanoparticles like ʻole, me nā nanoparticles hiki ke hoʻoheheʻe ʻia. Ma ka hoʻololi ʻana i nā ʻano hana centrifugation kuʻuna, ua hiki i nā mea noiʻi ke lanakila i nā pilikia e pili ana i ka solubility o nā nanoparticles a hoʻokō i ka hoʻokaʻawale pono. ʻO ka centrifugation gradient density, ka chromatography exclusion size, ultracentrifugation, a me ka density-gradient ultracentrifugation kekahi o nā hoʻolālā like ʻole i hoʻohana ʻia e hoʻokaʻawale pono i nā nanoparticles hiki ke hoʻoheheʻe ʻia. Hoʻomākaukau kēia mau ʻano hana i ke ala no nā holomua hou aʻe a me nā noi o ka nanotechnology ma nā ʻano like ʻole mai ka lāʻau lapaʻau a i ka ʻepekema kaiapuni.
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