Za a iya raba nanoparticles masu narkewa ta hanyar centrifugation

Gabatarwa

Centrifugation wata dabara ce da ake amfani da ita sosai a fannin fasahar nanotechnology don raba nau'ikan nanoparticles daban-daban bisa ga halayensu, kamar girma, siffa, da yawa. Musamman nanoparticles masu narkewa suna haifar da ƙalubale na musamman saboda ikonsu na narkewa ko warwatsewa a cikin ruwa. Wannan labarin ya yi nazari kan amfani da centrifugation a cikin rabuwa nanoparticles masu narkewa kuma ya bincika dabarun daban-daban da ake amfani da su don cimma rabuwa mai inganci.

Fahimtar Tsarin Tsaftacewa

Tsarin centrifugation tsari ne da ke amfani da ƙarfin centrifugal don raba ƙwayoyin cuta a cikin cakuda daban-daban. Ya ƙunshi jujjuya samfurin a cikin babban gudu, wanda ke haifar da ƙwayoyin cuta zuwa laka bisa ga yawansu. Ka'idar da ke bayan centrifugation ita ce ƙwayoyin cuta masu yawa za su zauna cikin sauri kuma su samar da kwaro a ƙasan bututun, yayin da ƙwayoyin cuta masu sauƙi za su kasance a cikin babban abin da ke cikinsa.

1. Dabaru na Gargajiya na Centrifugation

Dangane da raba ƙwayoyin nanoparticles masu narkewa, dabarun centrifugation na gargajiya ba za su iya samar da sakamako mai gamsarwa ba. Tunda ƙwayoyin nanoparticles masu narkewa suna narkewa ko warwatse a cikin ruwa, suna iya zama ƙalubale wajen lalata laka yadda ya kamata. Duk da haka, an ƙirƙiri wasu gyare-gyare da dama ga hanyoyin centrifugation na gargajiya don magance wannan matsalar.

2. Tsarin Nauyin Nauyin Nauyin Nauyi

Tsarin centrifugation mai yawa wata dabara ce da ake amfani da ita sosai don raba ƙwayoyin halitta masu irin wannan yawa. A yanayin ƙwayoyin halitta masu narkewa, ana iya daidaita matsakaicin yawa don daidaita yawan ƙwayoyin da ake so. Ta hanyar shimfida samfurin a hankali a kan wani tsari mai yawa da aka riga aka samar, ana iya raba ƙwayoyin halitta bisa ga yawansu yayin da suke ƙaura zuwa matsayin daidaito a cikin matsakaici.

3. Tsarin Chromatography Mai Keɓance Girma (SEC)

Tsarin Chromatography na Girman-Bangaren Halitta (SEC), wanda kuma aka sani da gel filtering chromatography, wata hanya ce da ake amfani da ita don raba ƙwayoyin halitta ko ƙwayoyin halitta bisa girmansu. A yanayin ƙwayoyin halitta masu narkewa, ana iya daidaita SEC don raba ƙwayoyin halitta bisa ga girmansu na hydrodynamic. Ƙananan ƙwayoyin halitta za su ratsa ramukan matakin da ba ya tsayawa, wanda ke haifar da tsawon lokacin riƙewa, yayin da manyan ƙwayoyin halitta za su ɓace da sauri.

4. Tsaftacewa ta Ultracent

Ultracentrifugation wani nau'i ne na ci gaba na centrifugation wanda ya ƙunshi saurin gudu mafi girma da kuma tsawon lokacin gudu fiye da hanyoyin gargajiya. Ana iya amfani da wannan dabarar don raba ƙananan ƙwayoyin halitta masu narkewa ta hanyar daidaita ƙarfin centrifugal da aka yi amfani da shi da kuma inganta lokacin gudu. Ta hanyar nemo mafi kyawun yanayi, ana iya raba ƙananan ƙwayoyin halitta masu narkewa yadda ya kamata bisa ga ma'aunin sedimentation ɗinsu, wanda ke ba da damar tattara ƙananan ƙwayoyin halitta masu niyya cikin inganci.

5. Ƙarfin Ɗaukar Nauyi Mai Sauƙi-Girman Ultra

Tsarin ultracentrifugation mai girman digiri da yawa ya haɗa ƙa'idodin centrifugation mai girman digiri da ultracentrifugation don raba ƙananan ƙwayoyin halitta masu narkewa. Ana kafa ƙaramin ƙwayar halitta ta amfani da matsakaici mai dacewa, kuma ana sanya samfurin a saman. Sannan ana yin ultracentrifugation don raba ƙananan ƙwayoyin halitta bisa ga yawansu da kuma cimma babban rabo. Wannan dabarar tana ba da damar raba daidai girma dabam-dabam ko nau'ikan ƙwayoyin halitta a cikin siffa mai narkewa.

Kammalawa

Dabaru na centrifugation sun tabbatar da cewa suna da matuƙar amfani wajen raba ƙwayoyin halitta daban-daban, gami da ƙwayoyin halitta masu narkewa. Ta hanyar gyara hanyoyin centrifugation na gargajiya, masu bincike sun sami damar shawo kan ƙalubalen da ke tattare da narkewar ƙwayoyin halitta da kuma cimma rabuwa mai inganci. centrifugation na gradient density, chromatography na cire girman abu, ultracentrifugation, da ultracentrifugation na density-gradient suna daga cikin dabarun da ake amfani da su don raba ƙwayoyin halitta masu narkewa yadda ya kamata. Waɗannan dabarun suna buɗe hanya don ci gaba da amfani da fasahar nanotechnology a fannoni daban-daban, tun daga magani zuwa kimiyyar muhalli.