ho ka arola li-nanoparticles tse qhibilihang ka har'a centrifugation

Selelekela

Ho Centrifugation ke mokhoa o sebelisoang haholo lefapheng la nanotechnology ho arola likaroloana tse fapaneng tsa nano ho latela thepa ea tsona, joalo ka boholo, sebopeho le bongata. Likaroloana tse qhibilihang, ka ho khetheha, li baka phephetso e ikhethang ka lebaka la bokhoni ba tsona ba ho qhibilihisa kapa ho hasana ka har'a mokelikeli o metsi. Sengoloa sena se hlahloba ts'ebeliso ea ho centrifugation karohanong ea likaroloana tse qhibilihang 'me se hlahloba maano a fapaneng a sebelisoang ho fihlela karohano e sebetsang hantle.

Ho utloisisa Centrifugation

Ho centrifugation ke mokhoa o sebelisang matla a centrifugal ho arola likaroloana ka motsoako o sa tšoaneng. Ho kenyelletsa ho potoloha sampole ka lebelo le phahameng, e leng se etsang hore likaroloana li be lerōle ho latela boima ba tsona. Molao-motheo o ka morao ho ho centrifugation ke hore likaroloana tse teteaneng li tla lula ka potlako 'me li etse pellet tlase ho tube, ha likaroloana tse bobebe li tla sala ka har'a supernatant.

1. Mekhoa ea Setso ea ho Fokotsa Matla

Tabeng ea ho arola likaroloana tse nyenyane tse qhibilihang, mekhoa ea setso ea ho centrifugation e kanna ea se hlahise liphetho tse khotsofatsang. Kaha likaroloana tse nyenyane tse qhibilihang li na le tšekamelo ea ho qhibiliha kapa ho hasana ka har'a mokelikeli o metsi, li ka ba thata ho li lahla ka katleho. Leha ho le joalo, ho entsoe liphetoho tse 'maloa mekhoeng ea setso ea ho centrifugation ho rarolla bothata bona.

2. Sekhahla sa Gradient sa Botenya

Ho centrifugation ha gradient e teteaneng ke mokhoa o sebediswang haholo ho arola dikarolwana tse nang le botenya bo tshwanang. Tabeng ya di-nanoparticles tse qhibilihang, density medium e ka fetolwa ho lekana le density e lakatsehang ya dikarolwana tse rerilweng. Ka ho beha sampole ka hloko hodima gradient e se e bopilwe esale pele ya density, di-nanoparticles di ka arolwa ho ya ka botenya ba tsona ha di ntse di fallela maemong a tsona a tekano kahare ho medium.

3. Kromatography ea ho se kenyelelloe boholo (SEC)

Chromatography ea ho Tlosa Boholo (SEC), e tsejoang hape e le gel filtration chromatography, ke mokhoa o tsebahalang o sebelisoang ho arola limolek'hule kapa likaroloana ho latela boholo ba tsona. Tabeng ea li-nanoparticles tse qhibilihang, SEC e ka fetoloa ho arola li-nanoparticles ka katleho ho latela boholo ba tsona ba hydrodynamic. Li-nanoparticles tse nyane li ne li tla kenella masobeng a karolo e sa sisinyeheng, e leng se lebisang ho nako e telele ea ho boloka, ha li-nanoparticles tse kholo li ne li tla tsoa kapele.

4. Ultracentrifugation

Ultracentrifugation ke mofuta o tsoetseng pele oa centrifugation o kenyeletsang lebelo le phahameng le linako tse telele tsa ho matha ho feta mekhoa e tloaelehileng. Mokhoa ona o ka sebelisoa ho arola li-nanoparticles tse qhibilihang ka ho fetola matla a centrifugal a sebelisitsoeng le ho ntlafatsa nako ea ho matha. Ka ho fumana maemo a matle, li-nanoparticles tse qhibilihang li ka aroloa ka katleho ho latela li-coefficients tsa tsona tsa sedimentation, e leng se lumellang pokello e sebetsang ea li-nanoparticles tse reriloeng.

5. Sekhahla-sekhahla sa Ultracentrifugation

Ho sebelisa ultracentrifugation ea gradient e nang le density-gradient ho kopanya melao-motheo ea density gradient centrifugation le ultracentrifugation ho arola li-nanoparticles tse qhibilihang. Ho thehoa gradient ea density ho sebelisoa mokhoa o loketseng, 'me sampole e beoa ka holimo. Ho sebelisoa ultracentrifugation ho arola li-nanoparticles ho latela density ea tsona le ho fihlela karohano e phahameng. Mokhoa ona o lumella karohano e nepahetseng ea boholo bo fapaneng kapa mefuta ea li-nanoparticles ka mokhoa o qhibilihang.

Qetello

Mekhoa ea ho centrifugation e ipakile e le ea bohlokoa haholo karohanong ea likaroloana tse fapaneng tsa nano, ho kenyeletsoa le likaroloana tse qhibilihang. Ka ho fetola mekhoa ea setso ea ho centrifugation, bafuputsi ba khonne ho hlola liphephetso tse amanang le ho qhibiliha ha likaroloana tse nyenyane le ho fihlela karohano e sebetsang hantle. Ho centrifugation ea gradient ea density, chromatography ea ho tlosa boholo, ultracentrifugation, le ultracentrifugation ea density-gradient ke tse ling tsa maano a fapaneng a sebelisoang ho arola likaroloana tse qhibilihang ka katleho. Mekhoa ena e bula tsela bakeng sa tsoelo-pele e eketsehileng le ts'ebeliso ea nanotechnology mafapheng a fapaneng ho tloha bongaka ho ea saense ea tikoloho.