yadda ake raba chloroplast da mitochondria ta hanyar centrifugation

Tsarin Na'urar Tsabtace Ƙasa: Wata Dabaru Mai Ƙarfi Don Raba Chloroplast da Mitochondria

Gabatarwa:

Centrifugation wata dabara ce da ake amfani da ita sosai a fannin ilmin kwayoyin halitta da kuma ilmin sunadarai wanda ke ba da damar raba sassan kwayoyin halitta daban-daban bisa girmansu, siffarsu, da kuma yawansu. Wannan labarin ya yi nazari kan amfani da centrifugation don raba chloroplasts da mitochondria, wasu kwayoyin halitta masu mahimmanci guda biyu da ake samu a cikin kwayoyin halitta na eukaryotic da ke da hannu a samar da makamashi da sauran muhimman hanyoyin tantanin halitta.

Matsayin Chloroplasts da Mitochondria a cikin Tsarin Kwayoyin Halitta

Chloroplasts, waɗanda ke cikin ƙwayoyin shuka, suna da alhakin photosynthesis, wani tsari wanda ke canza kuzarin haske zuwa makamashin sinadarai da aka adana a cikin nau'in glucose. Wannan muhimmin tsari yana sauƙaƙa samar da iskar oxygen kuma yana da mahimmanci don wadatar rayuwa a Duniya. Mitochondria, wanda ake samu a cikin ƙwayoyin shuka da na dabbobi, galibi ana kiransa da ƙarfin tantanin halitta. Suna da alhakin numfashin tantanin halitta, suna canza glucose zuwa makamashi mai amfani ga tantanin halitta a cikin nau'in adenosine triphosphate (ATP).

Bukatar Raba Chloroplasts da Mitochondria

Fahimtar ayyuka da halayen chloroplasts da mitochondria yana da matuƙar muhimmanci ga masu bincike da ke son samun fahimta game da hanyoyin ƙwayoyin halitta da kuma haɓaka hanyoyin magance cututtuka daban-daban. Raba waɗannan ƙwayoyin halitta daga cakuda hadaddun abubuwan da ke cikin ƙwayoyin halitta yana da matuƙar muhimmanci don samun samfuran tsabta don ƙarin bincike da gwaji. Wannan rabuwar tana yiwuwa ta hanyar centrifugation.

Muhimman bayanai game da centrifugation

Tsarin centrifugation ya ƙunshi amfani da ƙarfin centrifugal don raba abubuwan da ke cikin samfurin. Ana sanya samfurin a cikin injin centrifuge, injin da ke da rotor wanda ke juyawa a cikin babban gudu. A lokacin juyawa, ƙarfin centrifugal yana sa abubuwan da suka fi yawa su yi laka a ƙasa, suna samar da pellet, yayin da abubuwan da ba su da yawa ba su kasance a cikin supernatant. Saurin da tsawon lokacin centrifugation, da kuma zaɓin bututun centrifuge, sun bambanta dangane da takamaiman aikace-aikacen da abun da ke cikin samfurin.

Bambancin Centrifugation don Rabuwar Organelle

Hanyar centrifugation da aka fi amfani da ita don rabuwar organelle ita ce centrifugation mai bambanci. Wannan dabarar ta dogara ne akan ƙa'idar raba samfurin ta hanyar sanya shi zuwa jerin centrifugations masu bambanci a cikin ƙarin gudu da tsawon lokaci. Ta hanyar daidaita sigogin centrifugation, masu bincike za su iya raba chloroplasts da mitochondria yadda ya kamata daga sauran sassan tantanin halitta.

Jagorar Mataki-mataki don Raba Chloroplasts da Mitochondria

Mataki na 1: Haɗaka

Mataki na farko a cikin bambancin centrifugation shine daidaita samfurin. Wannan tsari ya ƙunshi karya ƙwayoyin halitta a hankali da kuma sakin abubuwan da ke cikinsu, wanda ke ba da damar shiga ga ƙwayoyin halitta. Ana iya amfani da hanyoyi da dama don wargaza ƙwayoyin halitta, kamar su wargaza injina da kuma maganin nitrogen mai ruwa-ruwa.

Mataki na 2: Bambance-bambancen Centrifugation

Bayan an haɗa su, ana yin amfani da samfurin a cikin jerin centrifugations daban-daban. Centrifugation na farko mai ƙarancin gudu (1000-2000 xg) yana cire manyan tarkacen ƙwayoyin halitta da ƙwayoyin da ba su karye ba, yana barin ƙaramin mitochondria da chloroplast. Wannan ɓangaren ana yin amfani da centrifugation mai sauri mafi girma (10,000-15,000 xg), wanda ke haifar da rabuwar chloroplasts daga mitochondria.

Mataki na 3: Inganta Tsarkaka

Domin ƙara tsarkake sassan chloroplast da mitochondria da aka samu, ana iya yin ƙarin matakan centrifugation. Wannan ya haɗa da sake ɗaura ƙwayar da aka samu bayan kowace centrifugation a cikin wani ma'ajiyar da ta dace da kuma maimaita aikin tare da ƙarin sauri da tsawon lokaci. Waɗannan ƙarin centrifugations za su taimaka wajen kawar da duk wani gurɓataccen abu da ya rage da kuma ƙara tsarkin sassan organelle na ƙarshe.

Kammalawa:

Centrifugation wata dabara ce mai amfani da yawa wadda ta kawo sauyi a fannin ilmin halittar tantanin halitta kuma ta zama kayan aiki mai mahimmanci don raba sassan tantanin halitta. Ta hanyar amfani da centrifugation daban-daban, masu bincike za su iya raba chloroplasts da mitochondria cikin nasara, suna samar da samfuran da aka tsarkake don ƙarin bincike da gwaji. Ikon ware waɗannan gabban halitta yana buɗe ƙofofi don nazarin takamaiman ayyukansu da hanyoyin su, yana ba da gudummawa ga fahimtar hanyoyin tantanin halitta da haɓaka ci gaba a fannoni daban-daban na kimiyya.