nzira yekuparadzanisa chloroplast ne mitochondria kuburikidza ne centrifugation

Kudzora Kutenderera: Nzira Ine Simba Yekuparadzanisa Chloroplast neMitochondria

Nhanganyaya:

Kubatanidza masero (centrifugation) inzira inoshandiswa zvakanyanya mu molecular biology ne biochemistry iyo inobvumira kupatsanurwa kwezvikamu zvakasiyana zvemasero zvichienderana nehukuru, chimiro, uye huwandu hwazvo. Chinyorwa chino chinotarisa kushandiswa kwekubatanidza masero (centrifugation) pakuparadzanisa ma chloroplast ne mitochondria, ma organelle maviri anokosha anowanikwa mumasero eukaryotic anobatanidzwa mukugadzirwa kwesimba uye mamwe maitiro emasero anokosha.

Basa reChloroplasts neMitochondria muMaitiro eSero

Ma chloroplast, ari mumasero emiti, anokonzera photosynthesis, maitiro anoshandura simba rechiedza kuita simba remakemikari rinochengetwa muchimiro cheglucose. Maitiro aya anobatsira kugadzirwa kweokisijeni uye akakosha pakutsigira hupenyu panyika. Mitochondria, inowanikwa mumasero emiti nemhuka, inowanzonzi simba resero. Anokonzera kufema kwemasero, achishandura glucose kuita simba rinoshanda resero muchimiro che adenosine triphosphate (ATP).

Kudiwa Kwekuparadzanisa Chloroplasts neMitochondria

Kunzwisisa mashandiro uye hunhu hwema chloroplast uye mitochondria kwakakosha kune vaongorori vane chinangwa chekuwana ruzivo rwemaitiro emasero uye kugadzira mishonga inogona kushandiswa pakurapa zvirwere zvakasiyana-siyana. Kuparadzanisa ma organelle aya kubva mumusanganiswa wakaoma wezvikamu zvemasero kwakakosha kuti uwane mienzaniso yakachena yekuongorora nekuyedza. Kupatsanurwa uku kunogoneka kuburikidza ne centrifugation.

Nheyo dzeCentrifugation

Kutenderedza zvinhu kunosanganisira kushandisa simba repakati kuparadzanisa zvikamu zviri mumuenzaniso. Muenzaniso wacho unoiswa mu centrifuge, muchina une rotor inotenderera nekumhanya kwakanyanya. Panguva yekutenderera, simba repakati rinoita kuti zvikamu zvakawandisa zviite sediment pasi, zvichigadzira pellet, nepo zvikamu zvisina kusimba zvichiramba zviri mu supernatant. Kumhanya uye nguva ye centrifuge, pamwe nesarudzo yemachubhu epakati, zvinosiyana zvichienderana nekushandiswa kwacho uye kuumbwa kwemuenzaniso.

Kusiyanisa kweCentrifugation yeOrganelle Separation

Nzira inonyanya kushandiswa ye centrifugation yekuparadzanisa organelles ndeye differential centrifugation. Iyi nzira yakavakirwa papfungwa yekupatsanura sampuro nekuiisa kune akatevedzana e differential centrifugations nekumhanya kwakawedzerwa uye kwenguva refu. Nekugadzirisa ma parameter e centrifugation, vaongorori vanogona kuparadzanisa zvinobudirira ma chloroplast nema mitochondria kubva kune zvimwe zvikamu zvemasero.

Nhungamiro Yenhanho Nhanho Yekuparadzanisa Chloroplasts neMitochondria

Danho 1: Kubatanidzwa kweHomogenization

Danho rekutanga mu differential centrifugation ndeye homogenization yemuenzaniso. Maitiro aya anosanganisira kupwanya masero zvinyoro nyoro uye kuburitsa zviri mukati mawo, zvichibvumira kupinda muma organelles. Nzira dzakasiyana siyana dzinogona kushandiswa pakuvhiringidza masero, dzakadai sekugadzirisa makemikari uye kurapa liquid nitrogen.

Danho rechipiri: Kusiyanisa Centrifugation

Mushure mekusanganiswa kwemasero, sampuro inoiswa muzvikamu zvakasiyana-siyana. Kuiswa muzvikamu zvishoma (1000-2000 xg) kunobvisa tsvina huru dzemasero nemasero asina kuputsika, zvichisiya mitochondria isina kucheneswa nechikamu chechloroplast. Chikamu ichi chinoiswa muzvikamu zvine kumhanya kwakanyanya (10,000-15,000 xg), zvichikonzera kuparadzaniswa kwema chloroplast kubva ku mitochondria.

Danho rechitatu: Kuvandudza Kuchena

Kuti zvinyatso chenesa chloroplast ne mitochondria fractions, mamwe matanho e centrifugation anogona kuitwa. Izvi zvinosanganisira kumisazve pellet yakawanikwa mushure me centrifugation yega yega mu buffer yakakodzera uye kudzokorora maitiro acho nekukurumidza uye kwenguva yakareba. Izvi zvinowedzera centrifugation zvichabatsira kubvisa chero tsvina yasara uye kuwedzera kuchena kwezvikamu zvekupedzisira zve organelle.

Mhedziso:

Kugadzira centrifugation inzira inoshanda zvakasiyana-siyana yakachinja munda webiology yemasero uye yave chishandiso chakakosha chekuparadzanisa zvikamu zvemasero. Nekushandisa differential centrifugation, vaongorori vanogona kuparadzanisa ma chloroplast nema mitochondria zvinobudirira, vachipa mienzaniso yakakosha yakacheneswa yekuongorora nekuyedza. Kukwanisa kuparadzanisa ma organelle aya kunovhura mikana yekudzidza mabasa avo chaiwo uye mashandiro, zvichibatsira kunzwisisa zviri nani maitiro emasero uye kukurudzira kufambira mberi mune dzakasiyana siyana dzesainzi.