Me pēhea te wehewehe i te chloroplast me te mitochondria mā te centrifugation
Te Whakapūngao: He Tikanga Kaha mō te Wehewehe i te Chloroplast me te Mitochondria
Kupu Whakataki:
He tikanga whānui te centrifugation i roto i te koiora ngota me te matū koiora e āhei ai te wehewehe i ngā wāhanga pūtau rerekē i runga i tō rātou rahi, āhua, me te mātotoru. Ka rukuhia e tēnei tuhinga te whakamahinga o te centrifugation hei wehewehe i ngā chloroplast me ngā mitochondria, arā, ngā whekau nui e rua e kitea ana i roto i ngā pūtau eukaryotic e uru ana ki te whakaputa pūngao me ētahi atu tukanga pūtau nui.
Te Tūranga o ngā Chloroplasts me ngā Mitochondria i roto i ngā Tukanga Pūtau
Ko ngā kōroporoporo, kei roto i ngā pūtau tipu, te kawenga mō te whakaahua-whakaahua, he tukanga e huri ai te pūngao mārama ki te pūngao matū e rongoatia ana i roto i te ahua o te hukahuka. Mā tēnei tukanga nui ka āwhina i te whakaputanga o te hāora, ā, he mea nui hoki mō te oranga tonutanga o te ora i runga i te Ao. Ko ngā mitochondria, kei roto i ngā pūtau tipu me ngā pūtau kararehe, e kiia whānuitia ana ko te whare hiko o te pūtau. Ko rātou te kawenga mō te manawa pūtau, te huri i te hukahuka hei pūngao e taea ai te whakamahi e te pūtau i roto i te ahua o te adenosine triphosphate (ATP).
Te Hiahia mō te Wehewehe i ngā Chloroplasts me ngā Mitochondria
He mea nui te mārama ki ngā mahi takitahi me ngā āhuatanga o ngā chloroplasts me ngā mitochondria mō ngā kairangahau e whai ana ki te whiwhi māramatanga ki ngā tukanga pūtau me te whakawhanake i ngā maimoatanga pea mō ngā mate maha. He mea nui te wehewehe i ēnei whekau mai i tētahi ranunga matatini o ngā wāhanga pūtau kia whiwhi tauira parakore mō ētahi atu tātaritanga me ngā whakamātautau. Ka taea tēnei wehewehenga mā te centrifugation.
Ngā Kaupapa Taketake o te Whakapūtanga
Ko te whakamahi i te kaha whakateretere hei wehewehe i ngā wāhanga o roto i tētahi tauira. Ka whakanohoia te tauira ki roto i tētahi mīhini whakateretere, he mīhini he rotor e hurihuri ana i ngā tere teitei. I te wā e hurihuri ana, ka meinga e te kaha whakateretere ngā wāhanga matotoru kia takoto ki raro, ka hanga he pirepire, ko ngā wāhanga iti ake te matotoru ka noho tonu ki roto i te wai mata. Ka rerekē te tere me te roa o te whakateretere, me te kōwhiringa o ngā ngongo whakateretere, i runga i te tono motuhake me te hanganga tauira.
Te Whakapūtanga Rerekē mō te Wehenga o ngā Organelle
Ko te tikanga whakapūmau e whakamahia whānuitia ana mō te wehenga o ngā whekau ko te whakapūmau rerekētanga. Ko tēnei tikanga e hangai ana ki te mātāpono o te wehewehe i te tauira mā te tuku i a ia ki roto i tētahi raupapa o ngā whakapūmau rerekētanga i ngā tere me ngā roa e piki haere ana. Mā te whakarerekē i ngā tawhā o te whakapūmau, ka taea e ngā kairangahau te wehewehe pai i ngā chloroplast me ngā mitochondria mai i ētahi atu wāhanga pūtau.
He Aratohu Hipanga-i-te-Hipanga ki te Wehewehe i ngā Chloroplasts me ngā Mitochondria
Hipanga 1: Te Whakaōrite
Ko te taahiraa tuatahi i roto i te centrifugation rerekētanga ko te whakakotahitanga o te tauira. Ko tēnei tukanga he wahi mārire i ngā pūtau me te tuku i ō rātou ihirangi, kia taea ai te uru atu ki ngā whekau. He maha ngā tikanga ka taea te whakamahi mō te whakararuraru i ngā pūtau, pērā i te whakararuraru ā-pūkaha me te maimoatanga hauota wai.
Hipanga 2: Te Whakapūtanga Rerekētanga
Whai muri i te whakakotahitanga, ka tukuna te tauira ki tētahi raupapa o ngā centrifugations rerekē. Mā te centrifugation tere-iti tuatahi (1000-2000 xg) ka tangohia ngā para nui o ngā pūtau me ngā pūtau kāore anō kia pakaru, ka waiho he mitochondria mata me te wāhanga chloroplast. Kātahi ka tukuna tēnei wāhanga ki tētahi centrifugation tere-teitei ake (10,000-15,000 xg), ka hua ake te wehenga o ngā chloroplasts mai i ngā mitochondria.
Hipanga 3: Whakapai Ake i te Parakore
Hei whakapai ake i ngā wāhanga chloroplast me mitochondria kua riro mai, ka taea te mahi i ētahi atu mahi centrifugation. Kei roto i tēnei ko te whakatārewatanga anō i te pellet i riro mai i muri i ia centrifugation ki roto i tētahi arai tika, me te whakahoki anō i te tukanga me te tere me te roa ake. Mā ēnei centrifugations tāpiri ka āwhina i te whakakore i ngā poke e toe ana, me te whakanui ake i te parakore o ngā wāhanga organelle whakamutunga.
Whakatau:
He tikanga maha ngā mahi a te centrifugation kua huri i te ao o te koiora pūtau, ā, kua waiho hei taputapu tino nui mō te wehewehe i ngā wāhanga pūtau. Mā te whakamahi i te centrifugation rerekē, ka taea e ngā kairangahau te wehewehe angitu i ngā chloroplast me ngā mitochondria, ka whakaratohia he tauira parakore whai hua mō te tātari me te whakamātautau anō. Mā te kaha ki te wehe i ēnei whekau ka whakatuwhera i ngā tatau mō te ako i ā rātou mahi me ngā tikanga motuhake, ka whai wāhi ki te mārama ake ki ngā tukanga pūtau me te whakatairanga i ngā whanaketanga i roto i ngā momo kaupapa pūtaiao.
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