yuav ua li cas cais cov chloroplast thiab mitochondria los ntawm centrifugation
Centrifugation: Ib Txoj Kev Siv Zog Rau Kev Sib Cais Chloroplast Thiab Mitochondria
Kev Taw Qhia:
Kev siv centrifugation yog ib txoj kev siv dav hauv kev tshawb fawb txog molecular biology thiab biochemistry uas ua rau muaj kev sib cais ntawm cov khoom sib txawv ntawm lub cev raws li lawv qhov loj me, cov duab, thiab qhov ceev. Tsab xov xwm no piav qhia txog kev siv centrifugation rau kev sib cais chloroplasts thiab mitochondria, ob lub cev tseem ceeb uas pom muaj nyob rau hauv eukaryotic hlwb uas koom nrog kev tsim hluav taws xob thiab lwm yam txheej txheem tseem ceeb ntawm lub cev.
Lub Luag Haujlwm ntawm Chloroplasts thiab Mitochondria hauv Cov Txheej Txheem Cellular
Cov Chloroplasts, uas muaj nyob rau hauv cov hlwb ntawm cov nroj tsuag, yog lub luag haujlwm rau photosynthesis, ib qho txheej txheem uas hloov lub zog lub teeb mus rau hauv lub zog tshuaj lom neeg khaws cia rau hauv daim ntawv ntawm glucose. Cov txheej txheem tseem ceeb no pab txhawb kev tsim cov pa oxygen thiab yog qhov tseem ceeb rau kev noj qab haus huv ntawm lub neej hauv ntiaj teb. Mitochondria, pom muaj nyob rau hauv ob qho tib si cov hlwb ntawm cov nroj tsuag thiab tsiaj txhu, feem ntau hu ua lub zog ntawm lub hlwb. Lawv yog lub luag haujlwm rau kev ua pa ntawm tes, hloov glucose mus rau hauv lub zog siv tau rau lub hlwb hauv daim ntawv ntawm adenosine triphosphate (ATP).
Qhov Xav Tau Rau Kev Sib Cais Chloroplasts Thiab Mitochondria
Kev nkag siab txog cov haujlwm thiab cov yam ntxwv ntawm chloroplasts thiab mitochondria yog qhov tseem ceeb rau cov kws tshawb fawb uas xav nkag siab txog cov txheej txheem ntawm tes thiab tsim cov kev kho mob rau ntau yam kab mob. Kev cais cov organelles no los ntawm cov khoom sib xyaw ua ke ntawm tes yog qhov tseem ceeb kom tau txais cov qauv ntshiab rau kev tshuaj xyuas thiab kev sim ntxiv. Qhov kev sib cais no ua tau los ntawm kev centrifugation.
Cov Ntsiab Lus Tseem Ceeb ntawm Kev Centrifugation
Kev siv lub zog centrifugal los cais cov khoom hauv cov qauv. Cov qauv raug muab tso rau hauv lub centrifuge, lub tshuab uas muaj lub rotor uas tig ceev heev. Thaum lub sijhawm tig, lub zog centrifugal ua rau cov khoom ntom ntom dua poob rau hauv qab, ua cov pellet, thaum cov khoom ntom ntom tsawg dua tseem nyob hauv supernatant. Qhov ceev thiab lub sijhawm ntawm kev siv centrifuge, nrog rau kev xaiv cov raj centrifuge, sib txawv nyob ntawm qhov kev siv thiab cov qauv sib xyaw.
Kev sib txawv ntawm Centrifugation rau Organelle Separation
Txoj kev centrifugation uas siv ntau tshaj plaws rau kev sib cais cov organelle yog differential centrifugation. Cov txheej txheem no yog raws li lub hauv paus ntsiab lus ntawm kev faib cov qauv los ntawm kev muab nws rau hauv ntau qhov sib txawv ntawm kev nce ceev thiab ntev. Los ntawm kev kho cov kev ntsuas ntawm centrifugation, cov kws tshawb fawb tuaj yeem cais cov chloroplasts thiab mitochondria ntawm lwm cov khoom ntawm tes.
Cov Kauj Ruam Qhia Txog Kev Sib Cais Chloroplasts thiab Mitochondria
Kauj Ruam 1: Kev Sib Koom Ua Ke
Kauj ruam thawj zaug hauv kev sib txawv centrifugation yog homogenization ntawm cov qauv. Cov txheej txheem no cuam tshuam nrog kev maj mam rhuav qhib cov cell thiab tso lawv cov ntsiab lus, tso cai rau nkag mus rau organelles. Muaj ntau txoj hauv kev siv tau rau kev cuam tshuam cell, xws li kev cuam tshuam tshuab thiab kev kho kua nitrogen.
Kauj Ruam 2: Kev Sib Tw Centrifugation
Tom qab ua kom homogenization tiav, cov qauv yuav raug centrifugations sib txawv. Thawj qhov centrifugation qeeb qeeb (1000-2000 xg) tshem tawm cov khib nyiab loj thiab cov hlwb tsis tawg, tawm hauv qab mitochondria nyoos thiab chloroplast feem. Cov feem no tom qab ntawd raug centrifugation ceev dua (10,000-15,000 xg), ua rau chloroplasts sib cais ntawm mitochondria.
Kauj Ruam 3: Kev Txhim Kho Kev Huv Si
Yuav kom ua kom cov chloroplast thiab mitochondria fractions huv dua, cov kauj ruam centrifugation ntxiv tuaj yeem ua tau. Qhov no suav nrog kev rov ua dua cov pellet tau txais tom qab txhua qhov centrifugation hauv lub buffer tsim nyog thiab rov ua cov txheej txheem nrog kev nrawm thiab lub sijhawm ntev dua. Cov centrifugations ntxiv no yuav pab tshem tawm cov khoom tsis huv uas tseem tshuav thiab ua kom cov organelle fractions kawg huv dua.
Xaus lus:
Kev siv centrifugation yog ib txoj kev siv tau ntau yam uas tau hloov pauv txoj kev tshawb fawb txog cell biology thiab tau dhau los ua ib qho cuab yeej tseem ceeb rau kev sib cais cov khoom sib xyaw ntawm cell. Los ntawm kev siv differential centrifugation, cov kws tshawb fawb tuaj yeem sib cais cov chloroplasts thiab mitochondria, muab cov qauv huv si rau kev tshuaj xyuas thiab kev sim ntxiv. Lub peev xwm los cais cov organelles no qhib qhov rooj rau kev kawm lawv cov haujlwm tshwj xeeb thiab cov txheej txheem, pab txhawb kev nkag siab zoo dua ntawm cov txheej txheem cellular thiab txhawb kev nce qib hauv ntau yam kev tshawb fawb.
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