Kodi ziwalo za maselo zimagawanika bwanji panthawi ya centrifugation

Kodi Zigawo za Maselo Zimasiyana Bwanji Panthawi ya Centrifugation?

Chiyambi:

Kugawa maselo m'maselo ndi njira yamphamvu yomwe imagwiritsidwa ntchito m'ma laboratories ofufuza ndi m'malo azachipatala kuti alekanitse zigawo za maselo kutengera kuchuluka kwawo. Njirayi imagwira ntchito yofunika kwambiri pophunzira kapangidwe ka maselo, kupatula ziwalo zinazake, komanso kufufuza ntchito zosiyanasiyana za maselo. Kumvetsetsa momwe ziwalo za maselo zimagawikira panthawi yogawa maselo m'maselo ndikofunikira kuti ofufuza apeze zigawo za maselo zoyeretsedwa ndikupeza chidziwitso cha ntchito zawo. M'nkhaniyi, tifufuza mfundo zomwe zili kumbuyo kwa kugawa maselo m'maselo ndi njira zomwe zigawo za maselo zimagawikirana panthawiyi.

I. Mfundo Zokhudza Kutsegula Maginito:

Musanakambirane za kulekanitsa maselo panthawi yogawa maselo, ndikofunikira kumvetsetsa mfundo zazikulu za njira iyi. Kugawa maselo kumadalira kugwiritsa ntchito mphamvu ya centrifugal, yopangidwa ndi rotor yozungulira mofulumira, kuti ilekanitse tinthu tating'onoting'ono kutengera kulemera kwawo ndi kuchuluka kwawo. Liwiro ndi nthawi ya kugawa maselo zimatsimikizira kuchuluka kwa kulekanitsidwa komwe kwapezeka.

II. Kusiyanitsa kwa Centrifugation:

Kugawanitsa maselo m'njira zosiyanasiyana ndi njira imodzi yodziwika bwino yomwe imagwiritsidwa ntchito pogawa zigawo zosiyanasiyana za selo. Njira imeneyi nthawi zambiri imaphatikizapo masitepe angapo ogawa maselo m'njira yowonjezera liwiro ndi nthawi. Gawo lililonse logawa maselo m'njira zosiyanasiyana limathandiza kusiyanitsa ndi kukulitsa ziwalo zinazake za selo kutengera kuchuluka kwa madzi omwe amalowa m'thupi.

III. Chiŵerengero cha kusefukira kwa nthaka:

Kuchuluka kwa sedimentation ya tinthu tating'onoting'ono ta selo kumatanthauza chizolowezi chake choyenda pansi pa chubu cha centrifuge pansi pa mphamvu yokoka, kapena molondola, mphamvu ya centrifugal. Kuchuluka kwa sedimentation kumakhudzidwa ndi zinthu zosiyanasiyana, kuphatikizapo kukula, mawonekedwe, kuchuluka, ndi kukhuthala kwa medium. Panthawi ya centrifugation, tinthu tating'onoting'ono tokhala ndi sedimentation yambiri timakhazikika mofulumira, zomwe zimapangitsa kuti tisiyane ndi zigawo zochepetsera sedimentation.

IV. Kutulutsa ndi Kugawa Zinthu Zofunika Kwambiri:

Pamene njira yogawanitsira zinthu m'maselo ikupita patsogolo, zigawo kapena magawo osiyanasiyana amayamba kupangika mu chubu. Gawo lomwe lili pansi pa chubu, lomwe limakhazikika ngati pellet, lili ndi zigawo zolemera kwambiri komanso zokhuthala kwambiri za maselo. Gawoli nthawi zambiri limakhala ndi ma nuclei, mitochondria, ndi ma organelle ena. Gawo lomwe lili pamwamba pa pellet, lodziwika kuti supernatant, lili ndi zigawo zopepuka monga cytoplasm, ribosomes, ndi mapuloteni osungunuka.

V. Kuchepetsa Mphamvu ya Maginito:

Kuchuluka kwa ma centrifugation ndi njira yapamwamba kwambiri yomwe imagwiritsidwa ntchito kupeza magawo a maselo oyeretsedwa kwambiri. Njirayi imadalira kupanga kuchuluka kwa ma centrifugation mkati mwa chubu cha ma centrifugation. Mwa kuyika mosamala kuchuluka kosiyanasiyana kwa mayankho, monga sucrose kapena cesium chloride, maselo amatha kulekanitsidwa kutengera kuchuluka kwawo koyandama.

VI. Kuchepetsa kwa Isopycnic:

Kugawa kwa isopycnic, komwe kumadziwikanso kuti equilibrium centrifugation, ndi njira ina yogawa kuchuluka kwa ma gradient. Mu njira iyi, chitsanzocho chimayikidwa pamwamba pa kuchuluka kwa ma density gradient, ndipo kugawa kwa centrifugation kumachitika mpaka tinthu tating'onoting'ono titafika pa mfundo yawo ya isopycnic. Pa nthawiyi, tinthu tating'onoting'ono timakhala mu gradient pamalo pomwe kuchuluka kwake kuli kofanana ndi kwa medium yozungulira. Kugawa kwa isopycnic centrifugation ndikothandiza kwambiri pofufuza ma nucleic acid, mapuloteni, ndi ma virus.

Mapeto:

Njira yogwiritsira ntchito centrifugation imapatsa ofufuza chida champhamvu chophunzirira ndikupeza magawo enaake a maselo. Mwa kumvetsetsa mfundo zomwe zimapangitsa kuti maselo alekanitsidwe panthawi yogwiritsira ntchito centrifugation, asayansi amatha kuyeretsa zigawo za maselo, kusanthula ntchito zawo, ndikuwululanso zinsinsi za sayansi ya maselo. Kaya pogwiritsa ntchito njira zosiyanasiyana zogwiritsira ntchito centrifugation kapena njira zochepetsera kuchuluka kwa maselo, centrifugation ikadali njira yofunika kwambiri m'munda wa sayansi ya maselo ndi maselo.