zvikamu zvemasero zvinoparadzaniswa sei panguva ye centrifugation

Zvikamu zveSero Zvinoparadzaniswa Sei Munguva yeCentrifugation

Nhanganyaya:

Kubatanidza masero (centrifugation) inzira ine simba inoshandiswa mumarabhoritari ekutsvagisa nekurapa kupatsanura zvikamu zvemasero zvichienderana nehuwandu hwazvo. Maitiro aya anoita basa guru mukudzidza maumbirwo emasero, kuparadzanisa maorganelle chaiwo, uye kuongorora mabasa akasiyana-siyana emasero. Kunzwisisa kuti zvikamu zvemasero zvinopatsanurana sei panguva yekusanganisa masero kwakakosha kuti vaongorori vawane zvikamu zvemasero zvakacheneswa uye vawane ruzivo rwemabasa avo. Muchinyorwa chino, tichaongorora misimboti iri shure kwekubatanidza masero uye nzira dzinoshandiswa kupatsanura zvikamu zvemasero panguva yekuita uku.

I. Nheyo dzeCentrifugation:

Usati wakurukura nezvekuparadzaniswa kwemasero panguva ye centrifugation, zvakakosha kunzwisisa misimboti mikuru yeiyi nzira. Centrifugation inoshandisa simba re centrifugal, rinogadzirwa ne rotor inotenderera nekukurumidza, kuparadzanisa zvidimbu zvichienderana nehuremu hwazvo uye huwandu hwazvo. Kumhanya uye nguva ye centrifugation zvinosarudza huwandu hwekuparadzaniswa kwakawanikwa.

II. Kusiyanisa kweCentrifugation:

Kuparadzanisa masero emasero ndeimwe yenzira dzinoshandiswa zvakanyanya kupatsanura zvikamu zvakasiyana zvesero. Iyi nzira inowanzo sanganisira matanho akatevedzana ekuwedzera kumhanya uye nguva. Danho rega rega rekuchenesa masero rinobatsira kupatsanura nekupfumisa zvikamu zvemasero zvichienderana nehuwandu hwazvo hwekudonha kwemasero.

III. Mwero weKucherwa kweMarara:

Mwero wekudonha kwechinhu chesero unoreva maitiro aro ekufamba achienda pasi pechubhu ye centrifuge pasi pesimba regiravhiti, kana kuti, simba re centrifugal. Mwero wekudonha kwechinhu unokonzerwa nezvinhu zvakasiyana-siyana, zvinosanganisira saizi, chimiro, huwandu, uye viscosity yechinhu chacho. Munguva yekudonha kwechinhu, zvinhu zvine mwero wekukwirira kwechinhu zvinogara pasi nekukurumidza, zvichiita kuti zviparadzaniswe nezvikamu zvinodonha zvishoma nezvishoma.

IV. Zvidimbu zvePelleting uye Supernatant:

Sezvo maitiro e centrifugation achifambira mberi, zvikamu zvakasiyana-siyana kana zvikamu zvinotanga kuumbwa muchubhu. Chikamu chiri pasi pechubhu, chinogara sepellet, chine zvikamu zvemasero zvinorema uye zvakawandisa. Chikamu ichi chinowanzo pfuma ne nuclei, mitochondria, nedzimwe organelles. Chikamu chiri pamusoro pepellet, chinozivikanwa se supernatant, chine zvikamu zvakareruka zvakaita se cytoplasm, ribosomes, uye mapuroteni anonyungudika.

V. Kudzora Kuwanda Kwemagiraidhi:

Kuyera density gradient centrifugation inzira yepamusoro inoshandiswa kuwana masero akacheneswa zvakanyanya. Nzira iyi inotsamira pakugadzirwa kwekuwedzera kwehuwandu mukati mechubhu ye centrifugation. Nekunyatsoisa huwandu hwakasiyana hwemhinduro, dzakadai se sucrose kana cesium chloride, masero anogona kuparadzaniswa zvichienderana nehuwandu hwawo hwemvura.

VI. Kudzora Kushanda Kwemasero muIsopycnic:

Isopycnic centrifugation, inozivikanwawo se equilibrium centrifugation, imwe nzira yekuparadzanisa density gradient. Munzira iyi, sampuro inoiswa pamusoro pe density gradient, uye centrifugation inoitwa kusvika zvidimbu zvasvika pa isopycnic point yazvo. Panguva iyi, zvidimbu zvese zvinogara mu gradient panzvimbo iyo density yazvo yakaenzana neye medium yakapoteredza. Isopycnic centrifugation inonyanya kubatsira pakuongorora nucleic acids, mapuroteni, uye mavhairasi.

Mhedziso:

Maitiro e centrifugation anopa vaongorori chishandiso chine simba chekudzidza nekuparadzanisa zvikamu zvemasero chaiwo. Nekunzwisisa misimboti iri shure kwekuparadzaniswa kwemasero panguva ye centrifugation, masayendisiti anogona kuchenesa zvikamu zvemasero, kuongorora mashandiro azvo, uye kuwedzera kuburitsa zvakavanzika zve biology yemasero. Ingave ichishandisa nzira dze differential centrifugation kana density gradient, centrifugation inoramba iri nzira yakakosha mumunda we cellular ne molecular biology.