yuav ua li cas cov khoom ntawm tes sib cais thaum lub sijhawm centrifugation
Yuav Ua Li Cas Cov Cell Sib Cais Thaum Lub Sijhawm Centrifugation
Kev Taw Qhia:
Kev siv centrifugation yog ib txoj kev siv zog heev uas siv rau hauv cov chaw tshawb fawb thiab cov chaw kho mob los cais cov khoom sib xyaw ua ke raws li lawv qhov ceev. Cov txheej txheem no ua lub luag haujlwm tseem ceeb hauv kev kawm txog cov qauv ntawm cov cell, cais cov organelles tshwj xeeb, thiab tshawb nrhiav ntau yam haujlwm ntawm cov cell. Kev nkag siab txog seb cov cell sib cais li cas thaum lub sijhawm centrifugation yog qhov tseem ceeb rau cov kws tshawb fawb kom tau txais cov cell fractions huv thiab nkag siab txog lawv lub luag haujlwm. Hauv tsab xov xwm no, peb yuav kawm txog cov ntsiab cai tom qab centrifugation thiab cov txheej txheem uas cov khoom sib xyaw ua ke sib cais thaum lub sijhawm.
I. Cov Ntsiab Cai ntawm Centrifugation:
Ua ntej tham txog kev sib cais ntawm cov cell thaum lub sijhawm centrifugation, nws yog ib qho tseem ceeb kom nkag siab txog cov ntsiab cai tseem ceeb ntawm cov txheej txheem no. Centrifugation vam khom kev siv lub zog centrifugal, tsim los ntawm lub rotor tig sai sai, los cais cov khoom me me raws li lawv qhov hnyav thiab qhov ceev. Qhov ceev thiab lub sijhawm ntawm centrifugation txiav txim siab qib kev sib cais ua tiav.
II. Kev Sib Tw Centrifugation Sib Txawv:
Kev sib txawv centrifugation yog ib qho ntawm cov txheej txheem feem ntau siv los cais cov khoom sib txawv ntawm lub cell. Cov txheej txheem no feem ntau cuam tshuam nrog ntau cov kauj ruam centrifugation ntawm kev nce ceev thiab ntev. Txhua kauj ruam centrifugation pab cais thiab nplua nuj cov khoom tshwj xeeb ntawm lub cell raws li lawv cov nqi sedimentation.
III. Tus Nqi Nqus Dej:
Qhov nrawm ntawm cov khoom me me hauv lub cellular yog hais txog nws txoj kev txav mus rau hauv qab ntawm lub raj centrifuge nyob rau hauv lub zog ntawm lub ntiajteb txawj nqus, lossis qhov tseeb dua, lub zog centrifugal. Qhov nrawm ntawm cov khoom me me yog cuam tshuam los ntawm ntau yam, suav nrog qhov loj me, cov duab, qhov ceev, thiab viscosity ntawm cov khoom nruab nrab. Thaum lub sijhawm centrifugation, cov khoom me me uas muaj cov nrawm siab dua yuav poob qis sai dua, ua rau lawv sib cais los ntawm cov khoom me me qeeb dua.
IV. Cov feem pua ntawm cov khoom sib xyaw thiab cov khoom sib xyaw ua ke:
Thaum cov txheej txheem centrifugation nce qib, cov txheej sib txawv lossis cov feem sib txawv pib tsim hauv lub raj. Cov feem sib txawv ntawm qab ntawm lub raj, uas nyob ua ib lub pellet, muaj cov khoom sib xyaw ua ke hnyav tshaj plaws thiab ntom tshaj plaws. Cov feem sib txawv no feem ntau muaj cov nuclei, mitochondria, thiab lwm yam organelles. Cov feem sib txawv saum toj no lub pellet, hu ua supernatant, muaj cov khoom sib dua xws li cytoplasm, ribosomes, thiab cov protein uas yaj tau.
V. Kev Siv Centrifugation Gradient Density:
Kev siv lub tshuab centrifugation uas muaj qhov ceev sib txawv yog ib txoj kev siv tshuab uas siv los ua kom tau cov cell fractions uas huv heev. Txoj kev no siv lub tshuab no los tsim kom muaj qhov ceev sib txawv hauv lub raj centrifugation. Los ntawm kev muab cov kua sib txawv sib txawv, xws li sucrose lossis cesium chloride sib xyaw ua ke, cov cell tuaj yeem sib cais raws li lawv qhov ceev uas ntab tau.
VI. Kev Siv Isopycnic Centrifugation:
Isopycnic centrifugation, tseem hu ua equilibrium centrifugation, yog lwm hom kev sib cais ntawm qhov ceev. Hauv txoj kev no, cov qauv raug muab tso rau saum qhov ceev, thiab centrifugation raug ua kom txog thaum cov khoom me me mus txog lawv qhov isopycnic point. Ntawm lub sijhawm no, txhua lub khoom me me nyob hauv qhov gradient ntawm qhov chaw uas nws qhov ceev sib npaug rau qhov nruab nrab ib puag ncig. Isopycnic centrifugation yog qhov tshwj xeeb tshaj yog rau kev tshuaj xyuas nucleic acids, proteins, thiab cov kab mob.
Xaus lus:
Cov txheej txheem ntawm centrifugation muab cov kws tshawb fawb nrog lub cuab yeej muaj zog los kawm thiab cais cov khoom tshwj xeeb ntawm lub cell. Los ntawm kev nkag siab txog cov ntsiab cai tom qab kev sib cais ntawm lub cell thaum lub sijhawm centrifugation, cov kws tshawb fawb tuaj yeem ua kom cov khoom ntawm lub cell huv si, tshuaj xyuas lawv cov haujlwm, thiab ntxiv qhov tsis paub meej ntawm lub cell biology. Txawm hais tias siv cov txheej txheem sib txawv centrifugation lossis cov txheej txheem density gradient, centrifugation tseem yog ib qho txheej txheem tseem ceeb hauv kev tshawb fawb txog cellular thiab molecular biology.
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