me pēhea te wehewehe o ngā wāhanga pūtau i te wā e whakamahia ana te centrifugation
Me pēhea te wehewehe i ngā wāhanga pūtau i te wā o te centrifugation
Kupu Whakataki:
He tikanga kaha te whakamahi i te centrifugation i roto i ngā taiwhanga rangahau me ngā wāhi hauora hei wehewehe i ngā wāhanga pūtau i runga i tō rātou kiato. He mahi nui tā tēnei tukanga ki te ako i ngā hanganga pūtau, te wehe i ngā whekau motuhake, me te tūhura i ngā mahi pūtau. He mea nui te mārama ki te wehewehenga o ngā wāhanga pūtau i te wā o te centrifugation kia whiwhi ai ngā kairangahau i ngā wāhanga pūtau kua purea, kia mārama ai hoki ki ō rātou mahi. I roto i tēnei tuhinga, ka rukuhia e mātou ngā mātāpono kei muri i te centrifugation me ngā tikanga e wehewehea ai ngā wāhanga pūtau i te wā o te tukanga.
I. Ngā Mātāpono o te Whakapūtanga:
I mua i te kōrero mō te wehenga pūtau i te wā o te centrifugation, he mea nui kia mārama ki ngā mātāpono taketake o tēnei tikanga. Ko te centrifugation e whakawhirinaki ana ki te whakamahinga o te kaha centrifugal, e whakaputaina ana e te rotor e hurihuri tere ana, hei wehewehe i ngā matūriki i runga i tō rātou taumaha me te mātotoru. Ko te tere me te roa o te centrifugation e whakatau ana i te taumata wehenga kua tutuki.
II. Te Whakapūtanga Rerekētanga:
Ko te whakapūtanga rerekē tētahi o ngā tikanga tino whakamahia hei wehewehe i ngā wāhanga rerekē o te pūtau. Ko tēnei tikanga he maha ngā mahi whakapūtanga i te tere me te roa e piki haere ana. Mā ia mahi whakapūtanga ka āwhina i te wehewehe me te whakarei ake i ngā wāhanga pūtau motuhake i runga i ō rātou tere whakatō.
III. Te Tere Whakatakotoranga:
Ko te tere o te whakatōtō o te matūriki pūtau e pā ana ki tōna ia ki te neke ki raro o te ngongo whakatōtō i raro i te kaha o te taumaha, i te tika ake rānei, te kaha whakatōtō. Ka pāngia te tere o te whakatōtō e ngā āhuatanga maha, tae atu ki te rahi, te āhua, te mātotoru, me te matotoru o te reo. I te wā o te whakatōtō, ka tere ake te taunga o ngā matūriki he nui ake ngā tere whakatōtō, ka taea ai te wehe i a rātou mai i ngā wāhanga whakatōtō puhoi.
IV. Ngā Wāhanga Whakarewa me te Whakapūtanga:
I te haere tonu o te tukanga centrifugation, ka tīmata te hanga o ngā paparanga motuhake, ngā wāhanga rānei i roto i te ngongo. Ko te wāhanga kei raro i te ngongo, e tau ana hei pellet, kei roto ko ngā wāhanga pūtau tino taumaha me te tino kikī. He maha ngā wā ka whakanuia tēnei wāhanga ki ngā karihi, ngā mitochondria, me ētahi atu whekau. Ko te wāhanga kei runga ake i te pellet, e mōhiotia ana ko te supernatant, kei roto ko ngā wāhanga māmā ake pērā i te cytoplasm, ngā ribosome, me ngā pūmua wairewa.
V. Te Whakapūtanga o te Rōrahi Kiato:
Ko te whakapūtanga i te pikinga matotoru he tikanga matatau ake e whakamahia ana hei whiwhi i ngā wāhanga pūtau tino parakore. Ka whakawhirinaki tēnei tikanga ki te whakaputa i tētahi pikinga matotoru e piki haere ana i roto i te ngongo whakapūtanga. Mā te whakatakoto āta i ngā kukū rerekē o ngā otinga, pērā i te sucrose, te cesium chloride rānei, ka taea te wehewehe i ngā pūtau i runga i tō rātou matotoru e pōkarekare ana.
VI. Te Whakapūtanga Isopycnic:
Ko te centrifugation isopycnic, e mōhiotia ana ko te centrifugation taurite, he momo tikanga wehewehe ira-mātotoru. I tēnei tikanga, ka whakatakotoria te tauira ki runga i te gradient mātotoru, ā, ka mahia te centrifugation kia tae rā anō ngā matūriki ki tō rātou pūwāhi isopycnic. I tēnei wā, ka tau ia matūriki ki te gradient i te tūranga e ōrite ana tōna mātotoru ki te mātotoru o te reo e karapoti ana. He tino whai hua te centrifugation isopycnic mō te tātari i ngā waikawa karihi, ngā pūmua, me ngā huaketo.
Whakatau:
Mā te tikanga o te centrifugation ka taea e ngā kairangahau te ako me te wehe i ngā wāhanga pūtau motuhake. Mā te mārama ki ngā mātāpono kei muri i te wehenga pūtau i te wā o te centrifugation, ka taea e ngā kaipūtaiao te pure i ngā wāhanga pūtau, te tātari i ā rātou mahi, me te wetewete atu i ngā mea ngaro o te koiora pūtau. Ahakoa te whakamahi i te centrifugation rerekētanga, i ngā tikanga pikinga mātotoru rānei, he tikanga nui tonu te centrifugation i roto i te mara o te koiora pūtau me te koiora ngota.
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