yadda sassan tantanin halitta ke rabuwa yayin centrifugation

Yadda Sassan Kwayar Halitta Ke Rarrabawa A Lokacin Tsaftacewa

Gabatarwa:

Centrifugation wata dabara ce mai ƙarfi da ake amfani da ita a dakunan gwaje-gwaje na bincike da wuraren likitanci don raba sassan tantanin halitta bisa ga yawansu. Wannan tsari yana taka muhimmiyar rawa wajen nazarin tsarin tantanin halitta, ware takamaiman ƙwayoyin halitta, da kuma bincika ayyuka daban-daban na tantanin halitta. Fahimtar yadda sassan tantanin halitta ke rabuwa yayin centrifugation yana da mahimmanci ga masu bincike su sami tsararren sassan tantanin halitta da kuma fahimtar rawar da suke takawa. A cikin wannan labarin, za mu zurfafa cikin ƙa'idodin da ke bayan centrifugation da hanyoyin da sassan tantanin halitta ke rabuwa yayin aikin.

I. Ka'idojin Centrifugation:

Kafin a tattauna rabuwar ƙwayoyin halitta yayin centrifugation, yana da mahimmanci a fahimci ƙa'idodin wannan dabarar. centrifugation ya dogara ne akan amfani da ƙarfin centrifugal, wanda rotor mai juyawa da sauri ya samar, don raba ƙwayoyin halitta bisa ga yawansu da yawansu. Sauri da tsawon lokacin centrifugation yana ƙayyade matakin rabuwar da aka cimma.

II. Bambancin Tsarin Tsaftacewa:

Tsarin centrifugation na bambancin abu yana ɗaya daga cikin hanyoyin da aka fi amfani da su don ware sassa daban-daban na tantanin halitta. Wannan dabarar yawanci tana ƙunshe da jerin matakan centrifugation a cikin ƙaruwar gudu da tsawon lokaci. Kowane matakin centrifugation yana taimakawa wajen raba da kuma wadatar da takamaiman sassan tantanin halitta bisa ga yawan centrifugation ɗinsu.

III. Yawan Zafi:

Yawan laka na ƙwayar halitta yana nufin yadda yake motsawa zuwa ƙasan bututun centrifuge a ƙarƙashin ƙarfin nauyi, ko kuma mafi daidai, ƙarfin centrifugal. Yawan laka yana shafar abubuwa daban-daban, ciki har da girma, siffa, yawa, da kuma danko na matsakaici. A lokacin centrifugation, ƙwayoyin da ke da yawan laka suna kwanciya da sauri, wanda ke ba da damar rabuwa da sassan laka a hankali.

IV. Fashewa da kuma Guraben da suka wuce gona da iri:

Yayin da tsarin centrifugation ke ci gaba, yadudduka ko ɓangarori daban-daban suna fara samuwa a cikin bututun. Kashi a ƙasan bututun, wanda ke zama a matsayin kashin, ya ƙunshi sassan ƙwayoyin halitta mafi nauyi da yawa. Wannan kashi galibi ana wadatar da shi da ƙwayoyin halitta, mitochondria, da sauran ƙwayoyin halitta. Kashi a sama da kashin, wanda aka sani da supernatant, ya ƙunshi sassa masu sauƙi kamar cytoplasm, ribosomes, da furotin mai narkewa.

V. Tsarin Nauyin Nauyin Nauyin Nauyi:

Tsarin centrifugation mai yawa wata hanya ce ta zamani da ake amfani da ita don samun sassan ƙwayoyin halitta masu tsafta. Wannan hanyar ta dogara ne akan samar da wani tsari na ƙaruwar yawan ƙwayoyin halitta a cikin bututun centrifugation. Ta hanyar haɗa nau'ikan mafita daban-daban a hankali, kamar sucrose ko cesium chloride, ana iya raba ƙwayoyin halitta bisa ga yawan su.

VI. Tsarin Sanyaya Isopycnic:

Tsarin centrifugation na Isopycnic, wanda kuma aka sani da daidaitaccen centrifugation, wani nau'in dabara ce ta rabuwar yawan gradient. A cikin wannan hanyar, ana sanya samfurin a saman gradient na yawan gradient, kuma ana yin centrifugation har sai barbashi sun isa wurin isopycnic ɗinsu. A wannan lokacin, kowane barbashi yana zaune a cikin gradient a matsayi inda yawansa yayi daidai da na matsakaicin da ke kewaye. Tsarin centrifugation na Isopycnic yana da amfani musamman don nazarin nucleic acid, sunadarai, da ƙwayoyin cuta.

Kammalawa:

Fasahar centrifugation tana bai wa masu bincike kayan aiki mai ƙarfi don yin nazari da kuma ware takamaiman sassan tantanin halitta. Ta hanyar fahimtar ƙa'idodin da ke bayan rabuwar tantanin halitta yayin centrifugation, masana kimiyya za su iya tsarkake sassan tantanin halitta, su bincika ayyukansu, da kuma ƙara warware asirin ilimin halittar tantanin halitta. Ko ta amfani da hanyoyin centrifugation daban-daban ko hanyoyin gradient mai yawa, centrifugation ya kasance muhimmiyar dabara a fannin ilmin halittar tantanin halitta da kwayoyin halitta.