Hana ʻoihana o ka mea hoʻokaʻawale centrifuge a me ka mea hana mīkini lāʻau lapaʻau - Zonelink

Pehea e hoʻokaʻawale ai nā ʻāpana o ke kelepona i ka wā o ka centrifugation

2023-08-12

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Pehea e hoʻokaʻawale ai nā ʻāpana kelepona i ka wā o ka Centrifugation

Hoʻolauna:

He ʻano hana ikaika ka centrifugation i hoʻohana ʻia ma nā keʻena noiʻi a me nā wahi lapaʻau e hoʻokaʻawale i nā ʻāpana cellular ma muli o ko lākou nui. He kuleana koʻikoʻi kēia kaʻina hana i ke aʻo ʻana i nā ʻano cell, ka hoʻokaʻawale ʻana i nā organelles kikoʻī, a me ka ʻimi ʻana i nā hana cellular like ʻole. He mea nui ka hoʻomaopopo ʻana i ke ʻano o ka hoʻokaʻawale ʻana o nā ʻāpana cell i ka wā centrifugation no nā mea noiʻi e loaʻa ai nā ʻāpana cell i hoʻomaʻemaʻe ʻia a loaʻa ka ʻike i kā lākou mau kuleana. Ma kēia ʻatikala, e ʻeli mākou i nā kumumanaʻo ma hope o ka centrifugation a me nā ʻano hana e hoʻokaʻawale ai nā ʻāpana cell i ka wā o ke kaʻina hana.

I. Nā Kumumanaʻo o ka Centrifugation:

Ma mua o ke kūkākūkā ʻana i ka hoʻokaʻawale ʻana o nā cell i ka wā o ka centrifugation, he mea nui e hoʻomaopopo i nā loina kumu o kēia ʻano hana. Hilinaʻi ka centrifugation i ka hoʻopili ʻana o ka ikaika centrifugal, i hana ʻia e kahi rotor e wili wikiwiki ana, e hoʻokaʻawale i nā ʻāpana ma muli o ko lākou nuipa a me ka nui. ʻO ka wikiwiki a me ka lōʻihi o ka centrifugation e hoʻoholo i ka pae o ka hoʻokaʻawale ʻana i loaʻa.

II. ʻOkoʻa Centrifugation:

ʻO ka centrifugation ʻokoʻa kekahi o nā ʻano hana maʻamau i hoʻohana ʻia e hoʻokaʻawale i nā ʻāpana like ʻole o kahi cell. Hoʻokomo pinepine kēia ʻenehana i kahi moʻo o nā ʻanuʻu centrifugation ma ka wikiwiki a me ka lōʻihi e hoʻonui ana. Kōkua kēlā me kēia ʻanuʻu centrifugation i ka hoʻokaʻawale a hoʻonui i nā ʻāpana cell kikoʻī e pili ana i kā lākou mau helu sedimentation.

III. Ka nui o ka Sedimentation:

ʻO ka nui o ka sedimentation o kahi ʻāpana cellular e pili ana i kona ʻano e neʻe ai i ka lalo o kahi paipu centrifuge ma lalo o ka ikaika o ke koʻikoʻi, a ʻoi aku ka pololei, ka ikaika centrifugal. Hoʻopili ʻia ka nui o ka sedimentation e nā kumu like ʻole, me ka nui, ke ʻano, ka nui, a me ka viscosity o ka medium. I ka wā o ka centrifugation, ʻoi aku ka wikiwiki o ka noho ʻana o nā ʻāpana me nā nui sedimentation kiʻekiʻe, e ʻae ana i ko lākou hoʻokaʻawale ʻana mai nā ʻāpana sedimenting lohi.

IV. Nā ʻāpana Pelleting a me Supernatant:

I ka holomua ʻana o ke kaʻina hana centrifugation, hoʻomaka nā papa a i ʻole nā hakina like ʻole e hana i loko o ka paipu. ʻO ka hapa ma lalo o ka paipu, kahi e noho ai ma ke ʻano he pellet, aia nā ʻāpana cellular kaumaha a paʻa loa. Hoʻonui pinepine ʻia kēia hapa me nā nuclei, mitochondria, a me nā organelles ʻē aʻe. ʻO ka hapa ma luna o ka pellet, i ʻike ʻia ʻo ka supernatant, aia nā ʻāpana māmā e like me ka cytoplasm, ribosomes, a me nā protein hiki ke hoʻoheheʻe ʻia.

V. Ke Keʻena Hoʻokaʻawale ʻana o ka Gradient Density:

ʻO ka centrifugation gradient density kahi ʻenehana holomua i hoʻohana ʻia e loaʻa ai nā ʻāpana cell i hoʻomaʻemaʻe nui ʻia. Hilinaʻi kēia ʻano hana i ka hana ʻana o kahi gradient o ka piʻi ʻana o ka density i loko o ka paipu centrifugation. Ma ka hoʻopili pono ʻana i nā ʻano like ʻole o nā hopena, e like me ka sucrose a i ʻole cesium chloride, hiki ke hoʻokaʻawale ʻia nā cell ma muli o ko lākou density buoyant.

VI. Keʻena Centrifugation Isopycnic:

ʻO ka centrifugation Isopycnic, i ʻike ʻia hoʻi ʻo ka centrifugation equilibrium, he ʻano ʻē aʻe ia o ke ʻano hana hoʻokaʻawale gradient density. Ma kēia ʻano hana, ua kau ʻia ka laʻana ma luna o ka gradient density, a ua hoʻokō ʻia ka centrifugation a hiki i nā ʻāpana i ko lākou wahi isopycnic. I kēia manawa, noho kēlā me kēia ʻāpana i loko o ka gradient ma kahi kūlana kahi i like ai kona density me ko ka medium a puni. He mea pono loa ka centrifugation Isopycnic no ka nānā ʻana i nā waikawa nucleic, nā protein, a me nā virus.

Hopena:

Hāʻawi ke ʻano hana o ka centrifugation i nā mea noiʻi i kahi mea hana ikaika e aʻo a hoʻokaʻawale i nā ʻāpana cell kikoʻī. Ma ka hoʻomaopopo ʻana i nā loina ma hope o ka hoʻokaʻawale ʻana o nā cell i ka wā o ka centrifugation, hiki i nā kānaka ʻepekema ke hoʻomaʻemaʻe i nā ʻāpana cellular, kālailai i kā lākou mau hana, a wehe hou i nā mea pohihihi o ka biology cell. Inā paha e hoʻohana ana i ka centrifugation differential a i ʻole nā ʻano density gradient, ʻo ka centrifugation kahi ʻenehana koʻikoʻi ma ke kahua o ka biology cellular a me ka molecular.