Pehea e hoʻokaʻawale ai ka Centrifuge i ke koko e hana i ka serum?
Hoʻolauna
He wai koʻikoʻi ke koko e lawe ana i nā meaʻai koʻikoʻi, ka oxygen, nā hormones, a me nā cell ma loko o ko kākou kino. ʻOiai mākou e hoʻopili pinepine i ke koko me kona kala ʻulaʻula, aia maoli ia i kekahi mau ʻāpana, me ka plasma, nā cell koko ʻulaʻula a me ke keʻokeʻo, a me nā platelets. ʻO ka serum, ma ka ʻaoʻao ʻē aʻe, ʻo ia ka ʻāpana moakaka a wai o ke koko e koe ma hope o ka hana ʻana o ka clotting. Loaʻa i ka serum nā protein like ʻole, nā antibodies, nā enzymes, a me nā hormones. No ka loaʻa ʻana o ka serum mai ke koko, hoʻohana ʻia kahi hana i kapa ʻia ʻo centrifugation. Ma kēia ʻatikala, e luʻu mākou i loko o ke kaʻina hana hoihoi o ke ʻano o ka centrifuge e hoʻokaʻawale ai i ke koko e hana i ka serum.
1. Ke Hoʻomaopopo ʻana i ka Centrifugation
ʻO ke centrifugation kahi hana mechanical e hoʻohana ana i ka ikaika centrifugal e hoʻokaʻawale i nā ʻāpana o kahi mea wai e pili ana i ko lākou nui. Hoʻohana nui ʻia ia ma nā ʻano ʻepekema a me nā ʻoihana lapaʻau like ʻole, me ka biology, kemika, a me nā keʻena hoʻokolohua lapaʻau. ʻO nā lako i hoʻohana ʻia i ka centrifugation ua kapa ʻia he centrifuge. Hoʻopololei kēia mīkini i nā laʻana ma nā wikiwiki kiʻekiʻe, e hoʻoulu ana i ka ikaika centrifugal e hiki ai ke kaʻina hana hoʻokaʻawale.
2. Ka Hoʻonohonoho ʻana o ke Koko
I mea e maopopo ai pehea e hoʻokaʻawale ai ke centrifugation i ke koko e loaʻa ai ka serum, he mea nui e hoʻomaopopo i ke ʻano kumu o ke koko. ʻO ke koko ka mea nui i haku ʻia me ka plasma, nā hunaola koko ʻulaʻula (erythrocytes), nā hunaola koko keʻokeʻo (leukocytes), a me nā platelets (thrombocytes). ʻO ka plasma ma kahi o 55% o ka nui o ke koko a he wai melemele ia i haku ʻia me ka wai, nā protein, nā hormones, nā electrolytes, a me nā meaʻai ʻē aʻe. ʻO ke koena 45% i haku ʻia me nā ʻāpana cellular—nā hunaola koko ʻulaʻula a me ke keʻokeʻo, a me nā platelets.
3. Ke Kaʻina Hana o ka Centrifugation
No ka hoʻokaʻawale ʻana i ke koko i loko o ke serum a me kāna mau ʻāpana cellular, ua hōʻiliʻili ʻia kahi hāpana koko i loko o kahi paipu i loaʻa kahi anticoagulant e pale ai i ka clotting. Hōʻoia kēia anticoagulant e noho mau ke koko i kahi kūlana wai i ka wā o ke kaʻina hana centrifugation. A laila hoʻokomo ʻia ka paipu i loko o ka centrifuge a milo i nā wikiwiki kiʻekiʻe. I ka wikiwiki ʻana o ka centrifuge, ua koi ʻia nā ʻāpana cellular paʻa, ʻo ia hoʻi nā ʻulaʻula koko a me ke keʻokeʻo, me nā platelets, i lalo o ka paipu ma muli o ka ikaika centrifugal.
4. Ka Density ʻokoʻa a me ke Kaʻina Hoʻokaʻawale
ʻO kekahi o nā mea koʻikoʻi i ke kaʻina hana hoʻokaʻawale ʻana, ʻo ia ka ʻokoʻa o ka nui o nā ʻāpana koko. He ʻokoʻa ka nui o kēlā me kēia ʻāpana, ka mea e hoʻoholo ai pehea e hoʻokaʻawale ai i ka wā o ka centrifugation. ʻOi aku ka nui o nā hunaola koko ʻulaʻula ma mua o nā hunaola koko keʻokeʻo a me nā platelets, e hoʻonoho ana iā lākou ma lalo o ka paipu. ʻO ka papa luna o ka paipu, nona ka plasma a me kekahi mau hunaola koko keʻokeʻo a me nā platelets i koe, ua omo ʻia e kiʻi i ka serum. A laila hana hou ʻia kēia papa e loaʻa ai ka serum maʻemaʻe, ʻaʻohe ona ʻāpana cellular.
5. Ka Hōʻiliʻili a me ka Mālama ʻana i ka Serum
Ke hoʻomoʻa ʻia ka papa luna, e hōʻiliʻili pono ʻia ke koena o ka serum e pale aku i kekahi haumia. He mea nui e lawelawe i ka serum i hōʻiliʻili ʻia me nā mea sterile e mālama i kona maʻemaʻe. Ma hope o ka hōʻiliʻili ʻana, hiki ke hoʻohana ʻia ka serum no nā hoʻokolohua hoʻokolohua like ʻole, nā kaʻina hana diagnostic, a me ka noiʻi lapaʻau. Pono e mālama ʻia ka serum ma nā mahana haʻahaʻa, ma kahi o -80°C, e mālama i kona ʻano biochemical a mālama iā ia no ka hoʻohana ʻana i ka wā e hiki mai ana.
Hopena
He kuleana koʻikoʻi ke kaʻina hana o ka centrifugation i ka hoʻokaʻawale ʻana i ke koko e loaʻa ai ka serum. Ma ka hoʻohana ʻana i ka ʻokoʻa o ka nui o nā ʻāpana koko a me ka hoʻopili ʻana i ka ikaika centrifugal, hoʻokaʻawale pono kahi centrifuge i ka plasma mai nā mea cellular e like me nā ʻulaʻula koko a me ke keʻokeʻo, a me nā platelets. ʻO ka serum hopena he ʻāpana waiwai nui i hoʻohana ʻia i nā noi lapaʻau a me nā ʻepekema he nui. ʻO ka hoʻomaopopo ʻana i ke kaʻina hana o ke ʻano o ka centrifuge e hoʻokaʻawale ai i ke koko e hana i ka serum he mea nui ia no nā kaʻina hana hoʻokolohua like ʻole a kōkua i ka holomua ʻana i ke kahua o ka lāʻau lapaʻau a me ka noiʻi.
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