sei centrifuge inoparadzanisa ropa kuti igadzire serum

Centrifuge inoparadzanisa sei ropa kuti rigadzire serum?

Nhanganyaya

Ropa imvura inokosha inotakura zvinovaka muviri zvakakosha, okisijeni, mahormone, uye masero mumuviri wedu wese. Kunyange zvazvo tichiwanzobatanidza ropa neruvara rwaro rutsvuku, rine zvikamu zvakati wandei, zvinosanganisira plasma, masero matsvuku nemachena eropa, uye maplatelet. Serum, kune rumwe rutivi, chikamu chakajeka uye chemvura cheropa chinosara mushure mekunge chagwamba. Serum ine mapuroteni akasiyana-siyana, ma antibodies, ma enzyme, uye mahormone. Kuti uwane serum kubva muropa, nzira inonzi centrifugation inoshandiswa. Muchinyorwa chino, tichaongorora maitiro anonakidza ekuti centrifuge inoparadzanisa sei ropa kuti rigadzire serum.

1. Kunzwisisa Centrifugation

Kutenderedza zvinhu (centrifugation) inzira yemakanika inoshandisa simba repakati (centrifugal force) kupatsanura zvikamu zvechinhu chemvura zvichibva pakuwanda kwazvo. Inoshandiswa zvakanyanya muzvikamu zvakasiyana-siyana zvesainzi nezvekurapa, kusanganisira biology, chemistry, uye makiriniki. Midziyo inoshandiswa mukuita kuti zvinhu zvitenderere inonzi centrifuge. Muchina uyu unotenderedza sampuli nekumhanya kwakanyanya, uchigadzira simba repakati rinogonesa nzira yekuparadzanisa zvinhu.

2. Kuumbwa kweRopa

Kuti unzwisise kuti kupatsanura ropa kunoparadzanisa sei kuti ropa riwane serum, zvakakosha kunzwisisa kuumbwa kweropa. Ropa rinonyanya kuumbwa neplasma, masero matsvuku eropa (erythrocytes), masero machena eropa (leukocytes), uye maplatelet (thrombocytes). Plasma inoumba inenge 55% yehuwandu hweropa uye imvura yero ine mvura, mapuroteni, mahormone, maelectrolyte, nezvimwe zvinovaka muviri. 45% yasara inoumbwa nezvikamu zvemasero—masero matsvuku nemachena eropa, pamwe nemaplatelet.

3. Maitiro eCentrifugation

Kuti ropa riparadzaniswe muropa nezvikamu zvaro zvemasero, sampu yeropa inounganidzwa muchubhu ine mushonga unodzivirira kuungana kweropa kudzivirira kuungana kweropa. Mushonga uyu unochengetedza ropa kuti rirambe riri mumvura panguva yekushanda kwemasero. Chubhu inozoiswa muchubhu yemasero uye inotenderedzwa nekumhanya kwakanyanya. Sezvo centrifuge ichikurumidza, zvikamu zvemasero zvine denderedzwa, kureva masero matsvuku nemachena eropa, pamwe chete nemaplatelet, zvinomanikidzwa kusvika pasi pechubhu nekuda kwesimba re centrifugal.

4. Kuwanda kweMusiyano uye Maitiro Ekuparadzanisa

Chimwe chezvinhu zvakakosha pakupatsanurwa kweropa imhando yeropa rakasiyana. Chikamu chimwe nechimwe chine huwandu hwakasiyana, izvo zvinosarudza kuti richapatsanurwa sei panguva yekupanurwa kweropa. Masero matsvuku eropa akawandisa kupfuura masero machena eropa nemaplatelet, zvichiita kuti agare pasi pechubhu. Chikamu chepamusoro chechubhu, chine plasma nemamwe masero machena eropa nemaplatelet akasara, chinofefeterwa kuti chiwane serum. Chikamu ichi chinozogadziriswazve kuti chiwane serum yakachena, isina zvikamu zvemasero.

5. Kuunganidzwa uye Kuchengetwa kweSerum

Kana jira repamusoro rapfurwa, seramu yasara inotorwa nokungwarira kudzivirira kusvibiswa. Zvakakosha kubata seramu yakaunganidzwa nezvinhu zvisina utachiona kuti irambe yakachena. Mushure mekutorwa, seramu inogona kushandiswa pakuongorora kwakasiyana-siyana murabhoritari, maitiro ekuongorora, uye kutsvakurudza kwezvokurapa. Seramu inofanira kuchengetwa patembiricha yakaderera, kazhinji inenge -80°C, kuchengetedza chimiro chayo chemakemikari uye kuichengetedza kuti ishandiswe mune ramangwana.

Mhedziso

Maitiro e centrifugation anoita basa guru mukuparadzanisa ropa kuti riwane serum. Nekushandisa differential density yezvikamu zveropa uye kushandisa centrifugal force, centrifuge inobudirira kuparadzanisa plasma kubva kuzvinhu zvemasero zvakaita semasero matsvuku nemachena eropa, pamwe nemaplatelet. Serum inobuda chinhu chakakosha chinoshandiswa mumabasa akawanda ekurapa nesainzi. Kunzwisisa maitiro e centrifuge yekuparadzanisa ropa kuti rigadzire serum kwakakosha kune akasiyana maitiro erabhoritari uye kunobatsira mukufambira mberi mumunda wekurapa nekutsvaga.