Me pēhea te wehe a te centrifuge i te toto hei hanga serum
Me pēhea te wehewehe a te centrifuge i te toto hei hanga serum?
Kupu Whakataki
He wai nui te toto e kawe ana i ngā matūkai nui, te hāora, ngā homoni, me ngā pūtau puta noa i ō tātou tinana. Ahakoa e hono pinepine ana tātou i te toto ki tōna tae whero, he maha ngā wāhanga o roto, tae atu ki te plasma, ngā pūtau toto whero me te toto mā, me ngā pereti toto. Ko te toto toto, i tētahi atu ringa, ko te wāhanga mārama me te wai o te toto e toe ana i muri i te totoka. Kei roto i te toto toto ngā momo pūmua, ngā paturopi, ngā whākōkī, me ngā homoni. Hei tiki i te toto toto mai i te toto, ka whakamahia he tukanga e kiia nei ko te centrifugation. I roto i tēnei tuhinga, ka rukuhia e tātou te tukanga whakamīharo o te wehewehe a te centrifuge i te toto hei hanga i te toto toto toto.
1. Te Mārama ki te Whakapūtanga
Ko te centrifugation he tukanga miihini e whakamahi ana i te kaha centrifugal hei wehewehe i ngā wāhanga o tētahi matū wai i runga i tōna mātotoru. He whānuitia te whakamahinga i roto i ngā mara pūtaiao me te hauora, tae atu ki te koiora, te matū, me ngā taiwhanga haumanu. Ko te taputapu e whakamahia ana i roto i te centrifugation ka kiia he centrifuge. Ka hurihia e tēnei mīhini ngā tauira i ngā tere teitei, ka whakaputa i te kaha centrifugal e āhei ai te tukanga wehenga.
2. Te Hanganga o te Toto
Hei mārama ki te wehewehe a te centrifugation i te toto hei whiwhi i te serum, he mea nui kia mārama ki te hanganga taketake o te toto. Ko te toto te mea nui ko te plasma, ngā toto whero (erythrocytes), ngā toto mā (leukocyte), me ngā platelets (thrombocytes). Tata ki te 55% o te rōrahi toto te plasma, ā, he wai kōwhai kei roto ko te wai, ngā pūmua, ngā homoni, ngā electrolytes, me ētahi atu matūkai. Ko te toenga 45% kei roto ko ngā wāhanga pūtau—ngā toto whero me ngā toto mā, tae atu ki ngā platelets.
3. Te Tukanga o te Whakapūngao
Hei wehewehe i te toto ki roto i te toto toto me ōna wāhanga pūtau, ka kohia he tauira toto ki roto i tētahi ngongo kei roto he matū ārai toto hei ārai i te totoka. Mā tēnei matū ārai toto ka noho tonu te toto i roto i te āhua wai i te wā o te tukanga whakapūtā. Kātahi ka whakanohoia te ngongo ki roto i te pūtā ka mirohia i ngā tere teitei. I te tere haere o te whakapūtā, ka akiakihia ngā wāhanga pūtau kiato ake, arā, ngā toto whero me ngā toto mā, me ngā pereti toto, ki raro i te ngongo nā te kaha whakapūtā.
4. Te Kīanga Rerekētanga me te Tukanga Wehewehenga
Ko tētahi o ngā mea nui o te tukanga wehenga ko te rerekētanga o te mātotoru o ngā wāhanga toto. He rerekē te mātotoru o ia wāhanga, e whakatau ana i te āhua o te wehenga i te wā e whakamahia ana te centrifugation. He mātotoru ake ngā pūtau toto whero i ngā pūtau toto mā me ngā platelets, ā, ka tau ki raro i te ngongo. Ko te paparanga o runga o te ngongo, kei roto ko te plasma me ētahi toenga o ngā pūtau toto mā me ngā platelets, ka tangohia kia puta ai te serum. Kātahi ka tukatukahia anō tēnei paparanga kia puta ai he serum parakore, kāore he wāhanga pūtau.
5. Te Kohikohi me te Rokiroki i te Huka
Kia mimitia te paparanga o runga, ka kohia marietia te toenga o te serum kia kore ai e poke. He mea nui kia whakamahia ngā rauemi parakore hei pupuri i tōna parakore. I muri i te kohi, ka taea te whakamahi i te serum mō ngā whakamātautau taiwhanga, ngā tikanga tātaritanga, me ngā rangahau hauora. Me penapena te serum ki ngā pāmahana iti, tata ki te -80°C, kia mau tonu ai tōna hanganga matū koiora me te tiaki mō te whakamahinga a meake nei.
Whakamutunga
He mea nui te mahi a te tukanga centrifugation ki te wehewehe i te toto hei whiwhi i te serum. Mā te whakamahi i te rerekētanga o te kiato o ngā wāhanga toto me te whakamahi i te kaha centrifugal, ka wehe angitu te centrifuge i te plasma mai i ngā huānga pūtau pēnei i ngā toto whero me ngā toto mā, tae atu ki ngā platelets. He wāhanga nui te serum e hua mai ana e whakamahia ana i roto i ngā tono hauora me te pūtaiao maha. He mea nui te mārama ki te tukanga o te wehewehe a te centrifuge i te toto hei hanga serum mō ngā tikanga taiwhanga maha, ā, ka whai wāhi ki ngā whanaketanga i roto i te mara o te rongoā me te rangahau.
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