Yuav ua li cas centrifuge cais cov ntshav kom ua serum
Yuav ua li cas Centrifuge cais cov ntshav kom ua tau serum?
Kev Taw Qhia
Ntshav yog ib yam kua tseem ceeb uas nqa cov as-ham tseem ceeb, oxygen, cov tshuaj hormones, thiab cov cell thoob plaws peb lub cev. Txawm hais tias peb feem ntau xav txog ntshav nrog nws cov xim liab, nws muaj ntau yam khoom, suav nrog plasma, cov qe ntshav liab thiab cov qe ntshav dawb, thiab platelets. Serum, ntawm qhov tod tes, yog qhov ntshiab thiab kua ntawm cov ntshav uas tseem nyob tom qab cov ntshav txhaws. Serum muaj ntau yam protein, cov tshuaj tua kab mob, cov enzymes, thiab cov tshuaj hormones. Txhawm rau kom tau txais serum los ntawm cov ntshav, ib txoj kev hu ua centrifugation siv. Hauv tsab xov xwm no, peb yuav nkag mus rau hauv cov txheej txheem zoo kawg nkaus ntawm yuav ua li cas centrifuge cais cov ntshav los ua serum.
1. Nkag Siab Txog Kev Siv Centrifugation
Kev centrifugation yog ib qho txheej txheem siv tshuab uas siv lub zog centrifugal los cais cov khoom ntawm cov kua raws li lawv qhov ceev. Nws yog siv dav hauv ntau qhov kev tshawb fawb thiab kev kho mob, suav nrog biology, chemistry, thiab cov chaw kuaj mob. Cov khoom siv siv hauv centrifugation hu ua centrifuge. Lub tshuab no tig cov qauv ntawm qhov ceev ceev, tsim lub zog centrifugal uas ua rau cov txheej txheem sib cais.
2. Cov Ntshav Muaj pes tsawg leeg
Yuav kom nkag siab txog kev siv centrifugation cais cov ntshav kom tau txais cov serum li cas, nws yog ib qho tseem ceeb kom nkag siab txog cov khoom xyaw tseem ceeb ntawm cov ntshav. Cov ntshav feem ntau yog tsim los ntawm plasma, cov qe ntshav liab (erythrocytes), cov qe ntshav dawb (leukocytes), thiab platelets (thrombocytes). Plasma ua rau kwv yees li 55% ntawm cov ntshav thiab yog cov kua daj uas muaj dej, cov protein, cov tshuaj hormones, electrolytes, thiab lwm yam khoom noj muaj txiaj ntsig. 45% seem yog tsim los ntawm cov khoom hauv lub cev - cov qe ntshav liab thiab dawb, nrog rau platelets.
3. Cov Txheej Txheem ntawm Centrifugation
Yuav kom cais cov ntshav ua cov ntshav thiab nws cov khoom hauv lub cev, cov ntshav yuav raug sau rau hauv ib lub raj uas muaj cov tshuaj anticoagulant kom tiv thaiv kev txhaws. Cov tshuaj anticoagulant no ua kom cov ntshav tseem nyob hauv cov kua thaum lub sijhawm centrifugation. Tom qab ntawd, lub raj raug muab tso rau hauv lub centrifuge thiab tig nrog kev kub ceev. Thaum lub centrifuge nrawm dua, cov khoom hauv lub cev ntom dua, uas yog cov qe ntshav liab thiab dawb, nrog rau cov platelets, raug yuam kom mus rau hauv qab ntawm lub raj vim yog lub zog centrifugal.
4. Qhov Sib Txawv Ceev thiab Cov Txheej Txheem Sib Cais
Ib qho ntawm cov yam tseem ceeb hauv cov txheej txheem sib cais yog qhov sib txawv ntawm cov khoom xyaw ntawm cov ntshav. Txhua yam khoom muaj qhov sib txawv ntawm qhov sib txawv, uas txiav txim siab tias nws yuav sib cais li cas thaum lub sijhawm centrifugation. Cov qe ntshav liab muaj qhov ntom dua li cov qe ntshav dawb thiab cov platelets, ua rau lawv nyob hauv qab ntawm lub raj. Txheej sab saud ntawm lub raj, uas muaj cov plasma thiab qee cov qe ntshav dawb thiab cov platelets seem, raug nqus kom tau cov serum. Txheej no tom qab ntawd raug ua tiav ntxiv kom tau cov serum ntshiab, tsis muaj cov khoom xyaw ntawm tes.
5. Kev Sau thiab Khaws Cov Tshuaj Ntshav
Thaum cov txheej sab saud raug nqus tawm lawm, cov serum seem yuav tsum tau sau kom zoo kom tsis txhob muaj kev sib kis. Nws yog ib qho tseem ceeb uas yuav tsum tau siv cov serum uas tau sau los nrog cov khoom siv sterile kom nws huv si. Tom qab sau, cov serum tuaj yeem siv rau ntau yam kev kuaj hauv chaw kuaj mob, cov txheej txheem kuaj mob, thiab kev tshawb fawb kho mob. Cov serum yuav tsum tau khaws cia rau qhov kub qis, feem ntau nyob ib puag ncig -80 ° C, kom nws cov tshuaj biochemical muaj pes tsawg leeg thiab khaws cia rau yav tom ntej.
Xaus lus
Cov txheej txheem ntawm centrifugation ua lub luag haujlwm tseem ceeb hauv kev cais cov ntshav kom tau txais cov serum. Los ntawm kev siv qhov sib txawv ntawm cov khoom ntawm cov ntshav thiab siv lub zog centrifugal, lub centrifuge ua tiav kev cais cov plasma ntawm cov ntsiab lus ntawm tes xws li cov qe ntshav liab thiab cov qe ntshav dawb, nrog rau cov platelets. Cov serum uas tau los ntawm cov serum yog ib qho khoom muaj nqis siv rau ntau yam kev siv kho mob thiab kev tshawb fawb. Kev nkag siab txog cov txheej txheem ntawm centrifuge cais cov ntshav kom ua cov serum yog qhov tseem ceeb rau ntau yam txheej txheem hauv chaw kuaj mob thiab pab txhawb rau kev nce qib hauv kev kho mob thiab kev tshawb fawb.
.