Centrifuge inoparadzanisa sei zvinhu zvakasimba kubva mumvura nekukurumidza kudaro

Nhanganyaya yeCentrifugation

Kugadzira centrifugation inzira inoshandiswa zvakanyanya mumaindasitiri akasiyana-siyana uye munzvimbo dzekutsvagisa pakuparadzanisa zvinhu zvakasimba kubva mumvura zvinobudirira. Inzira inoshandisa misimboti yesimba re centrifugal kuparadzanisa zvikamu zvakasiyana zvichienderana nehuwandu hwazvo kana saizi. Muchinyorwa chino, tichaongorora mashandiro ari kuita kuti centrifuge ikwanise kuparadzanisa nekukurumidza kudaro, pamwe nekuongorora mashandisirwo ayo nekufambira mberi kwayo.

Nheyo Inoshanda yeCentrifuge

Centrifuge ine rotor inotenderera uye chibatiso chemuenzaniso, chinozivikanwa sechubhu yecentrifuge. Kana rotor ikatenderera nekumhanya kwakanyanya, inogadzira simba recentrifugal rinoita basa rekunze. Simba iri rinoita kuti zvidimbu kana zvinhu zvakawandisa zvibve pakati pekutenderera zvigare pasi pechubhu, ukuwo zvinhu zvakareruka zvichiramba zviri pedyo nepamusoro.

Kumhanya kunotenderera rotor chinhu chakakosha pakuona kushanda zvakanaka kwecentrifuge. Kumhanya kwakakwira kwekutenderera, ndiko kusimba kwecentrifugal. Saka, centrifuges dzakagadzirirwa kushanda nekumhanya kwakanyanya, kazhinji kubva pazviuru zvishoma zvekuchinja paminiti (RPM) kusvika kumakumi ezviuru.

Kucherwa kwemvura muCentrifugal

Imwe yenzira huru iyo centrifuge inoparadzanisa zvinhu zvakasimba kubva mumvura inonzi centrifugal sedimentation. Maitiro aya akavakirwa pakusiyana-siyana kwezvikamu zviri mumuenzaniso.

Kana sampuro ikaiswa muchubhu ye centrifuge yozopetwa, zvinhu zvakasimba zvakanyanya - zvakaita sezvidimbu, masero, kana marara - zvinokurumidza kugara pasi pechubhu nekuda kwesimba rakasimba re centrifugal. Panguva iyi, supernatant yemvura inoramba iri pamusoro, zvichiita kuti zvive nyore kubvisa kana kubvisa.

Kudzora Kuwanda Kwemagiraidhi

Kunyange zvazvo centrifugal sedimentation ichishanda zvakanaka pakuparadzanisa zvidimbu zvikuru, zvikamu zvidiki uye zvakapfava zvinogona kuda nzira yakasiyana. Apa ndipo panoshanda density gradient centrifugation.

Kushandiswa kwe density gradient centrifugation kunosanganisira kushandisa density gradient medium yakagadzirwa zvakanaka, inowanzo gadzirwa ne sucrose kana iodixanol. Medium yacho yakanyatsoiswa mu centrifuge tube, zvichiita kuti density gradient iwedzere zvishoma nezvishoma kubva kumusoro kusvika pasi.

Kana sampuro ikaiswa muchubhu yozopetwa, zvikamu zvinofamba nepakati pe density gradient kusvika zvasvika pakureba kwayo. Iyi nzira inobvumira kupatsanurwa kwezvidimbu kana zvinhu zvine density dzakafanana asi mwero we sedimentation wakasiyana.

Mashandisirwo eCentrifugation

Kumhanya uye kushanda zvakanaka kwe centrifugation kwakaita kuti ishandiswe zvakanyanya mumaindasitiri akasiyana-siyana. Mumarabhoritari ekurapa, centrifuges zvishandiso zvakakosha zvekuparadzanisa plasma kana serum kubva muropa, kuparadzanisa DNA, kana kugadzirira masero.

Muindasitiri yemishonga, centrifugation inobatsira mukuchenesa mishonga, yakaita se insulin kana antibiotics, nekuparadzanisa tsvina kubva kune zvinhu zvinoshanda. Inoshandiswawo mu biotechnology pakugadzira mishonga yakawanda yekudzivirira zvirwere, mapuroteni akasanganiswa, uye biofuels.

Uyezve, centrifugation inoita basa rakakosha mune zvesainzi yezvakatipoteredza uye yekuongorora mafambiro emhepo. Inobatsira mukuongorora ivhu, mvura, nemhepo nekuparadzanisa zvidimbu zvakamiswa kana zvinosvibisa kuti zviongororwe zvakanyanya.

Kufambira Mberi muTekinoroji yeCentrifugation

Mumakore apfuura, tekinoroji ye centrifugation yafambira mberi zvikuru. Ma centrifuge anomhanya zvakanyanya ave madiki, anoshanda zvakanaka, uye ane masisitimu ekudzora akasimba anokwanisa kubata huwandu hwakawanda hwemasampuli uku achichengetedza zviyero zvekuparadzanisa zvakanyatsonaka.

Pamusoro pezvo, kufambira mberi mumagadzirirwo erotor, akadai semarotor ane angle yakatarwa, marotor anotenderera, kana marotor akamira, zvinoita kuti zvive nyore kuchinjika kune mhando dzakasiyana dzesamples uye mavhoriyamu. Kuvandudzwa uku kwakasimudzira zvakanyanya kushanduka-shanduka uye kumhanya kwekuparadzanisa kunogona kuitwa ne centrifuge.

Mukupedzisa, centrifugation inzira inokosha zvikuru yekuparadzanisa zvinhu zvakasimba kubva mumvura mumaindasitiri akasiyana-siyana uye munzvimbo dzekutsvagisa. Ingave kuburikidza ne centrifugal sedimentation kana density gradient centrifugation, maitiro aya anogonesa kupatsanurwa kwakanaka uye anorerutsa kuongororwa uye kugadziriswa kwezvikamu zvinodiwa. Nekufambira mberi kuri kuenderera mberi, centrifugation inoramba ichikosha mune zvesainzi neindasitiri zvemazuva ano.