lub centrifuge cais cov khoom khov thiab cov kua sai npaum li cas

Kev Taw Qhia Txog Centrifugation

Kev siv centrifugation yog ib txoj kev siv dav hauv ntau yam lag luam thiab kev tshawb fawb rau kev sib cais cov khoom khov thiab cov kua kom zoo. Nws yog ib qho txheej txheem uas siv cov ntsiab cai ntawm lub zog centrifugal los cais cov khoom sib txawv raws li lawv qhov ceev lossis qhov loj. Hauv tsab xov xwm no, peb yuav tshawb nrhiav cov txheej txheem tom qab lub centrifuge tuaj yeem ua tiav kev sib cais sai li cas, nrog rau kev nkag mus rau hauv nws cov ntawv thov thiab kev nce qib.

Lub hauv paus ntsiab lus ua haujlwm ntawm lub centrifuge

Lub centrifuge muaj ib lub rotor tig thiab ib lub sampling holder, feem ntau hu ua centrifuge tube. Thaum lub rotor tig ntawm qhov ceev ceev, nws tsim ib lub zog centrifugal uas ua rau sab nraud radially. Lub zog no ua rau cov khoom me me lossis cov khoom me me txav deb ntawm qhov chaw ntawm kev tig thiab nyob hauv qab ntawm lub raj, thaum cov khoom sib dua tseem nyob ze rau sab saum toj.

Qhov ceev uas lub rotor tig yog qhov tseem ceeb hauv kev txiav txim siab qhov ua tau zoo ntawm kev sib cais ntawm lub centrifuge. Qhov ceev ntawm kev tig siab dua, lub zog centrifugal muaj zog dua. Yog li ntawd, centrifuges tau tsim los ua haujlwm ntawm qhov ceev heev, feem ntau yog txij li ob peb txhiab revolutions ib feeb (RPM) mus rau ntau kaum txhiab.

Kev Tso Tawm Centrifugal

Ib qho ntawm cov txheej txheem tseem ceeb uas lub centrifuge cais cov khoom khov thiab cov kua hu ua centrifugal sedimentation. Cov txheej txheem no yog raws li qhov sib txawv ntawm cov khoom muaj nyob hauv cov qauv.

Thaum muab cov qauv tso rau hauv lub raj centrifuge thiab tig, cov khoom khov dua - xws li cov khoom me me, cov cell, lossis cov khib nyiab - sai sai poob rau hauv qab ntawm lub raj vim yog lub zog centrifugal muaj zog. Lub sijhawm ntawd, cov kua supernatant tseem nyob rau sab saum toj, ua rau yooj yim rau kev decantation lossis tshem tawm.

Kev Siv Centrifugation Gradient Density

Txawm hais tias kev siv centrifugal sedimentation ua haujlwm zoo rau kev cais cov khoom loj dua, cov khoom me me thiab cov khoom uas yooj yim dua yuav xav tau txoj hauv kev sib txawv. Qhov no yog qhov uas kev sib xyaw ua ke ntawm qhov ceev thiab qhov sib txawv los ua si.

Kev centrifugation gradient density gradient siv cov khoom siv density gradient uas tau npaj tshwj xeeb, feem ntau yog muaj sucrose lossis iodixanol. Cov khoom siv no raug muab tso rau hauv lub raj centrifuge kom zoo, ua rau muaj qhov density gradient uas maj mam nce ntxiv los ntawm sab saud mus rau hauv qab.

Thaum cov qauv raug ntxiv rau hauv lub raj thiab tig, cov khoom sib xyaws yuav txav mus los ntawm qhov sib txawv ntawm qhov ceev kom txog thaum lawv mus txog lawv qhov ceev uas ntab. Cov txheej txheem no tso cai rau kev sib cais cov khoom me me lossis cov tshuaj uas muaj qhov ceev zoo sib xws tab sis cov nqi sib txawv ntawm kev tso dej.

Cov ntawv thov ntawm Centrifugation

Qhov ceev thiab ua haujlwm tau zoo ntawm kev centrifugation tau ua rau nws siv tau zoo hauv ntau yam lag luam. Hauv cov chaw kuaj mob, centrifuges yog cov cuab yeej tseem ceeb rau kev cais cov plasma lossis serum ntawm cov qauv ntshav, cais DNA, lossis npaj cov kab lis kev cai ntawm tes.

Hauv kev lag luam tshuaj, centrifugation pab ua kom cov tshuaj huv, xws li insulin lossis tshuaj tua kab mob, los ntawm kev cais cov khoom tsis huv ntawm cov tshuaj nquag. Nws kuj tseem siv rau hauv biotechnology rau kev tsim cov tshuaj tiv thaiv kab mob, cov protein recombinant, thiab cov roj biofuels loj.

Tsis tas li ntawd xwb, kev siv centrifugation ua lub luag haujlwm tseem ceeb hauv kev tshawb fawb txog ib puag ncig thiab kev tshawb fawb txog kev ua txhaum cai. Nws pab hauv kev tshuaj xyuas cov qauv av, dej, thiab huab cua los ntawm kev cais cov khoom me me lossis cov pa phem rau kev tshawb nrhiav ntxiv.

Kev nce qib hauv kev siv tshuab centrifugation

Tau ntau xyoo los no, cov thev naus laus zis centrifugation tau dhau los ua kev nce qib tseem ceeb. Cov centrifuges ceev ceev tau dhau los ua compact dua, ua haujlwm tau zoo dua, thiab nruab nrog cov txheej txheem tswj hwm zoo uas muaj peev xwm tswj cov qauv loj thaum tswj cov kev sib cais meej.

Tsis tas li ntawd xwb, kev nce qib hauv kev tsim cov rotor, xws li cov rotors uas muaj lub kaum sab xis, cov rotors uas tig mus rau hauv lub thoob, lossis cov rotors ntsug, muab kev hloov pauv ntau dua rau ntau hom qauv thiab ntim. Cov kev txhim kho no tau ua rau muaj kev hloov pauv ntau yam thiab qhov ceev ntawm kev sib cais uas lub centrifuge tuaj yeem ua tiav.

Xaus lus, centrifugation yog ib txoj kev tseem ceeb rau kev sib cais cov khoom khov thiab cov kua sai sai hauv ntau yam lag luam thiab kev tshawb fawb. Txawm yog los ntawm centrifugal sedimentation lossis density gradient centrifugation, cov txheej txheem no ua rau muaj kev sib cais zoo thiab pab txhawb kev tshuaj xyuas ntxiv thiab kev ua cov khoom xav tau. Nrog rau kev nce qib tas mus li, centrifugation tseem yog qhov tseem ceeb hauv kev tshawb fawb niaj hnub no thiab kev lag luam.