Me pēhea te tere o te wehewehe a te centrifuge i ngā totoka mai i ngā wai?

He Kupu Whakataki ki te Whakapūtanga

He tikanga whānui te whakamahi i te centrifugation i roto i ngā ahumahi me ngā mara rangahau hei wehewehe i ngā mea totoka mai i ngā wai. He tukanga e whakamahi ana i ngā mātāpono o te kaha centrifugation hei wehewehe i ngā wāhanga rerekē i runga i te matotoru, i te rahi rānei. I roto i tēnei tuhinga, ka tūhuratia e mātou ngā tikanga o te centrifugation hei whakatutuki i tētahi wehenga tere pēnei, me te ruku hohonu ki ōna tono me ōna whanaketanga.

Te Mātāpono Mahi o te Pūwhakarewa

Kei roto i te pūwero he rotor hurihuri me te pupuri tauira, e mōhiotia whānuitia ana ko te ngongo pūwero. Ina hurihuri te rotor i ngā tere teitei, ka puta he kaha pūwero e mahi ana ki waho. Mā tēnei kaha ka neke atu ngā matūriki, ngā matū rānei kia matotoru ake i te pokapū hurihuri ka tau ki raro o te ngongo, ko ngā matū māmā ake ka noho tata ki runga.

He mea nui te tere o te hurihuri hei whakatau i te pai o te wehenga o te pūwero. Ka teitei ake te tere hurihuri, ka kaha ake te kaha o te pūwero. Nō reira, kua hangaia ngā pūwero kia mahi i ngā tere tino teitei, mai i te mano tini hurihanga ia meneti (RPM) ki te tekau mano.

Te Whakatōtōtanga Pūwero

Ko tētahi o ngā tikanga matua e wehea ai e te centrifuge ngā totoka mai i ngā wai ko te centrifugal sedimentation. E hangai ana tēnei tukanga ki ngā momo matotoru o ngā wāhanga kei roto i te tauira.

Ina whakanohoia te tauira ki roto i te ngongo whakateretere, ā, ka mirohia, ka tere te taunga o ngā mea totoka kikī – pērā i ngā matūriki, ngā pūtau, ngā para rānei – ki raro i te ngongo nā te kaha whakateretere. I taua wā, ka noho tonu te wai i te tihi, kia ngāwari ai te ringihia, te tangohia rānei.

Te Whakapūtanga o te Rerenga Matotoru

Ahakoa he pai te mahi a te whakapūtātanga porowhita mō te wehewehe i ngā matūriki nunui, tera pea me rerekē te huarahi e hiahiatia ana mō ngā wāhanga iti ake, ngawari ake hoki. Koinei te wāhi e whai wāhi mai ai te porowhitatanga pikinga mātotoru.

Ko te whakapūtanga i te pikinga matotoru e pā ana ki te whakamahinga o tētahi rauemi whakapūtanga matotoru kua whakaritea motuhaketia, he mea hanga i te sucrose, i te iodixanol rānei. Ka ata whakatakotoria te rauemi ki roto i te ngongo whakapūtanga, ka hanga he pikinga matotoru e piki haere ana mai i runga ki raro.

Ina tāpirihia te tauira ki te ngongo, ā, ka mirohia, ka heke ngā wāhanga mā roto i te pikinga kiato kia tae rā anō ki ō rātou kiato pōrewarewa. Mā tēnei tikanga ka taea te wehewehe i ngā matūriki, i ngā matū rānei he rite te kiato engari he rerekē te tere o te whakatō.

Ngā Whakamahinga o te Whakapūtanga

Nā te tere me te whai huatanga o te centrifugation kua nui ngā whakamahinga i roto i ngā momo ahumahi. I roto i ngā taiwhanga hauora, he taputapu tino nui ngā centrifuges mō te wehewehe i te plasma, i te serum rānei mai i ngā tauira toto, te wehe i te DNA, te whakarite rānei i ngā ahurea pūtau.

I roto i te umanga rongoā, ka āwhina te centrifugation ki te pure i ngā rongoā, pērā i te insulin, i ngā paturopi rānei, mā te wehe i ngā paru mai i ngā matū hohe. Ka whakamahia hoki i roto i te hangarau koiora mō te hanga nui o ngā kano kano, ngā pūmua recombinant, me ngā wahie koiora.

Hei tāpiritanga, he mea nui te mahi a te centrifugation i roto i ngā pūtaiao taiao me ngā pūtaiao taihara. Ka āwhina i te tātari i ngā tauira oneone, wai, me te hau mā te wehewehe i ngā matūriki iri, i ngā poke rānei mō ētahi atu rangahau.

Ngā Whakatairanga i roto i te Hangarau Centrifugation

I roto i ngā tau, kua tino whanake haere te hangarau centrifugation. Kua iti ake, kua whai hua ake ngā centrifuges tere-rahi, ā, kua rite rawa ki ngā pūnaha whakahaere matatau ka taea te whakahaere i ngā rahinga tauira nui me te pupuri tonu i ngā tawhā wehenga tika.

Hei tāpiritanga, ko ngā whanaketanga o ngā hoahoa rotor, pērā i ngā rotor koki-pūmau, ngā rotor peere-swinging, ngā rotor poutū rānei, ka nui ake te urutau ki ngā momo tauira me ngā rahinga rerekē. Nā ēnei whakapainga kua tino whakarei ake i te whānui me te tere o te wehenga ka taea e te centrifuge te whakatutuki.

Hei whakamutunga, he tikanga tino nui te whakamahi i te centrifugation hei wehewehe tere i ngā mea totoka mai i ngā wai i roto i ngā momo ahumahi me ngā mara rangahau. Ahakoa mā te centrifugation whakatakotoranga, mā te centrifugation pikinga mātotoru rānei, ka taea e tēnei tukanga te wehewehe pai, ā, ka āwhina i te tātari me te tukatuka anō i ngā wāhanga e hiahiatia ana. Me ngā whanaketanga tonu, kei te whakaatu tonu te centrifugation he mea nui tonu i roto i ngā mahi pūtaiao me ngā mahi ahumahi o ēnei rā.