zomwe zimalekanitsa mu centrifuge

Kodi Centrifuge imalekanitsa chiyani ndi magetsi?

Chiyambi

Kutsegula magetsi ndi njira yodziwika bwino yopangira zinthu zomwe zimaphatikizapo kulekanitsa zigawo zosiyanasiyana za chisakanizo kutengera kuchuluka kwawo. Njirayi imagwiritsa ntchito mphamvu ya centrifugal kuti ipange mphamvu yokoka, zomwe zimapangitsa kuti tinthu tolemera tisunthire pansi pomwe topepuka timakhala pamwamba. Makina otsegula magetsi, makina opangidwira cholinga ichi, amagwira ntchito yofunika kwambiri pakulekanitsa zinthu m'magawo osiyanasiyana kuphatikiza kafukufuku wazachipatala, zamoyo, ndi mafakitale. M'nkhaniyi, tiphunzira mozama za lingaliro la centrifugation, momwe imagwiritsidwira ntchito, komanso njira zomwe zimailola kulekanitsa zigawo zosiyanasiyana bwino.

I. Kumvetsetsa Kuzungulira kwa Mitsempha

Kutsegula mlengalenga ndi njira yomwe imagwiritsa ntchito mfundo ya kusungunula, komwe tinthu tating'onoting'ono kapena zinthu zosiyanasiyana zimalekanitsidwa ndi momwe zimayankhira ku mphamvu ya centrifugal. Kuti izi zitheke, chitsanzo chimayikidwa mu rotor, yomwe imazungulira mofulumira kwambiri. Mphamvu ya centrifugal yomwe imachokera ku rotor imayambitsa kusungunula kwa tinthu tating'onoting'ono, zomwe zimawasiyanitsa bwino ndi zosakaniza zina zonse.

II. Mitundu ya Ma Centrifuge

1. Ma ultracentrifuge

Ma centrifuge a Ultracentrifuge ndi ma centrifuge othamanga kwambiri omwe amatha kufika pa liwiro lofika mpaka 150,000 rpm (rpm). Amagwiritsa ntchito ma rotor okhala ndi magawo apadera kuti alekanitse tinthu tating'onoting'ono kutengera kukula ndi kuchuluka kwawo. Ma centrifuge a Ultracentrifuge amagwiritsidwa ntchito m'magawo osiyanasiyana monga biology ya molecular, biochemistry, ndi virology.

2. Ma Microcentrifuge

Ma microcentrifuge ndi mitundu yaying'ono ya ma centrifuge omwe amapangidwira kufalitsa mwachangu ma volumes ang'onoang'ono. Amagwiritsidwa ntchito kwambiri mu ma molecular biology ndi ma biochemistry labotale pa ntchito monga kuyeretsa DNA ndi mapuloteni, kusonkhanitsa ma cell pellets, komanso kusinthasintha kwachangu kwa machubu otumizira ma centrifuge.

III. Kugwiritsa Ntchito Centrifugation

1. Kulekanitsa Zigawo za Magazi

Chimodzi mwa ntchito zodziwika bwino za centrifugation ndi kulekanitsa zigawo za magazi. Mu ma laboratories azachipatala, centrifuges amagwiritsidwa ntchito kulekanitsa plasma, maselo ofiira a magazi, ndi maselo oyera a magazi kuchokera ku zitsanzo zamagazi athunthu. Kulekanitsa kumeneku ndikofunikira kwambiri pa mayeso ozindikira matenda, kulemba magazi, komanso kukonzekera zinthu zinazake zamagazi, monga ma platelet concentrates.

2. Kulekanitsa DNA ndi RNA

Kutsegula ma centrifugation ndi chida chofunikira kwambiri pa kafukufuku wa zamoyo zama molekyulu chifukwa zimathandiza kulekanitsa bwino DNA ndi RNA kuchokera ku zitsanzo zosiyanasiyana. Mwa kutseka ma centrifugation awa pa liwiro ndi nthawi yeniyeni, zinthu za majini zimatha kulekanitsidwa ndi zigawo zina za maselo, zomwe zimathandiza asayansi kuphunzira ndikuwunika momwe DNA imagwirira ntchito kapena momwe majini amagwirira ntchito.

3. Kulekanitsa Zikhalidwe za Maselo

Mu sayansi ya zamoyo ndi sayansi ya zamoyo, kugawa maselo pogwiritsa ntchito centrifugation kumagwiritsidwa ntchito kulekanitsa ndikuyika zinthu za maselo kapena maselo athunthu kuchokera kuzinthu zokulira. Njirayi imathandiza pa ntchito zosiyanasiyana monga kusonkhanitsa maselo, kuyeretsa mapuloteni, ndi kulekanitsa ziwalo za maselo kuti ziwunikidwenso.

IV. Njira Yoyendetsera Kuzungulira

Kugawa kwa centrifugation kumagwira ntchito motsatira mfundo ya mphamvu ya centrifugal yomwe imakankhira tinthu tokhuthala kwambiri m'mphepete mwa rotor, zomwe zimapangitsa kuti pakhale dothi. Zinthu zofunika kwambiri zomwe zimakhudzidwa ndi njira yolekanitsira ndi izi:

1. Machubu a Rotor ndi Centrifuge

Rotor imazungulira mozungulira mzere, ndikupanga mphamvu ya centrifugal. Imasunga machubu a centrifuge, omwe ali ndi chisakanizo chomwe chiyenera kulekanitsidwa. Mitundu yosiyanasiyana ya ma rotor ndi machubu imapangidwa kuti igwirizane ndi kuchuluka kwa zitsanzo, kukula, ndi mphamvu ya centrifugal yomwe imafunika.

2. Liwiro ndi Kutalika

Liwiro ndi nthawi yogwiritsira ntchito centrifugation zimadalira mtundu wa chitsanzocho ndi kulekanitsidwa komwe mukufuna. Zipangizo zosiyanasiyana, monga maselo kapena tizilombo toyambitsa matenda, zingafunike liwiro ndi nthawi zosiyanasiyana kuti pakhale kulekanitsidwa koyenera.

Mapeto

Mwachidule, centrifuge ndi njira yofunika kwambiri yogwiritsidwa ntchito kulekanitsa zigawo zosiyanasiyana za chisakanizo kutengera kuchuluka kwawo. Ma centrifuge, kaya ndi ma ultracentrifuge kapena ma microcentrifuge, ndi zida zofunika kwambiri m'ma laboratories m'magawo osiyanasiyana, zomwe zimathandiza asayansi kupeza kulekanitsa koyenera pa ntchito zosiyanasiyana. Kuyambira kulekanitsa zigawo za magazi mpaka kuphunzira DNA ndi RNA mpaka kukonza maselo, centrifuge imagwira ntchito yofunika kwambiri pakupititsa patsogolo kafukufuku wasayansi ndi matenda. Pomvetsetsa njira zomwe centrifuge imagwirira ntchito komanso momwe imagwiritsidwira ntchito mosiyanasiyana, asayansi akupitilizabe kutsegula mwayi watsopano wopeza zinthu zatsopano komanso kupita patsogolo m'magawo osiyanasiyana.