dab tsi sib cais hauv lub centrifuge
Dab tsi sib cais hauv lub centrifuge?
Kev Taw Qhia
Kev centrifugation yog ib txoj kev kuaj mob uas siv ntau heev uas muaj kev sib cais cov khoom sib txawv ntawm cov khoom sib xyaw raws li lawv qhov ceev. Cov txheej txheem no siv lub zog centrifugal los tsim kom muaj lub zog gravitational, uas ua rau cov khoom hnyav dua txav mus rau hauv qab thaum cov khoom sib dua nyob rau sab saum toj. Lub centrifuge, lub tshuab tsim tshwj xeeb rau lub hom phiaj no, ua lub luag haujlwm tseem ceeb hauv kev sib cais cov khoom hauv ntau qhov chaw suav nrog kev kho mob, kev tshawb fawb txog tsiaj txhu, thiab kev lag luam. Hauv tsab xov xwm no, peb yuav kawm tob dua txog lub tswv yim ntawm centrifugation, nws cov ntawv thov, thiab cov txheej txheem uas tso cai rau nws sib cais cov khoom sib txawv tau zoo.
I. Kev Nkag Siab Txog Kev Centrifugation
Kev centrifugation yog ib txoj kev siv lub hauv paus ntsiab lus ntawm kev tso dej tawm, qhov twg cov khoom me me lossis cov khoom uas muaj qhov sib txawv sib cais los ntawm lawv cov lus teb rau lub zog centrifugal. Txhawm rau ua tiav qhov no, ib qho qauv raug muab tso rau hauv lub rotor, uas tig ntawm qhov ceev ceev. Lub zog centrifugal uas tsim los ntawm lub rotor ua rau cov khoom me me uas ntom dua tso dej tawm, ua rau lawv sib cais ntawm cov khoom sib xyaw seem.
II. Hom Centrifuges
1. Ultracentrifuges
Cov tshuab centrifuges ultracentrifuges yog cov tshuab centrifuges ceev ceev uas tuaj yeem ncav cuag qhov ceev txog li 150,000 revolutions ib feeb (rpm). Lawv siv cov rotors nrog cov chaw tshwj xeeb los cais cov khoom me me raws li lawv qhov loj me thiab qhov ceev. Ultracentrifuges pom cov ntawv thov hauv ntau qhov chaw xws li molecular biology, biochemistry, thiab virology.
2. Cov tshuab me me uas siv lub tshuab centrifuge
Cov tshuab centrifuge me me yog cov tshuab centrifuge me me uas tsim los rau kev tso dej sai sai ntawm cov ntim me me. Lawv siv dav hauv cov chaw soj nstuam molecular biology thiab biochemistry rau cov haujlwm xws li kev ntxuav DNA thiab protein, kev sau cov cell pellet, thiab kev tig cov raj centrifugation sai.
III. Cov Kev Siv ntawm Centrifugation
1. Kev Cais Cov Cheebtsam Ntshav
Ib qho ntawm cov kev siv centrifugation feem ntau yog kev sib cais cov khoom ntawm cov ntshav. Hauv cov chaw kuaj mob, centrifuges siv los cais cov plasma, cov qe ntshav liab, thiab cov qe ntshav dawb ntawm cov qauv ntshav tag nrho. Qhov kev sib cais no yog qhov tseem ceeb rau kev kuaj mob, kev ntaus cov ntshav, thiab kev npaj cov khoom ntshav tshwj xeeb, xws li cov platelet concentrates.
2. Kev sib cais ntawm DNA thiab RNA
Kev centrifugation ua pov thawj tias yog ib qho cuab yeej tseem ceeb hauv kev tshawb fawb txog molecular biology vim nws ua rau muaj kev sib cais zoo ntawm DNA thiab RNA los ntawm ntau yam qauv. Los ntawm kev centrifuging cov qauv no ntawm qhov ceev thiab lub sijhawm tshwj xeeb, cov khoom siv caj ces tuaj yeem sib cais los ntawm lwm cov khoom hauv lub cell, uas tso cai rau cov kws tshawb fawb kawm thiab tshuaj xyuas cov kab ke DNA lossis cov lus qhia gene.
3. Kev Sib Cais Cov Kab Mob Cell
Hauv kev tshawb fawb txog lub hlwb thiab biotechnology, centrifugation yog siv los cais thiab sib sau ua ke cov khoom ntawm lub hlwb lossis tag nrho cov hlwb ntawm cov khoom loj hlob. Cov txheej txheem no pab rau ntau yam kev siv xws li kev sau cov hlwb, kev ntxuav cov protein, thiab kev sib cais cov organelles ntawm lub hlwb rau kev tshuaj xyuas ntxiv.
IV. Lub Tshuab Centrifugation
Kev siv lub tshuab centrifugal ua haujlwm raws li lub hauv paus ntsiab lus ntawm lub zog centrifugal uas thawb cov khoom me me mus rau ntug sab nraud ntawm lub rotor, ua rau muaj kev sib xyaw ua ke. Cov khoom tseem ceeb uas koom nrog hauv cov txheej txheem sib cais yog:
1. Cov raj xa dej thiab cov raj xa dej Centrifuge
Lub rotor tig ib ncig ntawm ib lub axis, tsim lub zog centrifugal. Nws tuav cov raj centrifuge, uas muaj cov khoom sib xyaw kom sib cais. Ntau hom rotors thiab cov raj sib txawv tau tsim los kom haum rau cov ntim ntawm cov qauv, qhov loj me, thiab lub zog centrifugal uas xav tau.
2. Kev Ceev thiab Lub Sijhawm
Qhov ceev thiab lub sijhawm ntawm centrifugation nyob ntawm qhov xwm txheej ntawm cov qauv thiab qhov kev sib cais xav tau. Cov ntaub ntawv sib txawv, xws li cov cell lossis cov kab mob me me, yuav xav tau qhov ceev thiab lub sijhawm sib txawv kom ua tiav qhov kev sib cais zoo tshaj plaws.
Xaus lus
Hauv kev xaus lus, centrifugation yog ib txoj kev tseem ceeb uas siv los cais cov khoom sib txawv ntawm cov khoom sib xyaw raws li lawv qhov ceev. Centrifuges, txawm tias ultracentrifuges lossis microcentrifuges, yog cov cuab yeej tseem ceeb hauv chaw soj nstuam thoob plaws ntau qhov chaw, uas tso cai rau cov kws tshawb fawb kom ua tiav kev sib cais zoo rau ntau yam kev siv. Txij li kev cais cov khoom ntshav mus rau kev kawm DNA thiab RNA mus rau kev ua cov kab lis kev cai ntawm tes, centrifugation ua lub luag haujlwm tseem ceeb hauv kev txhim kho kev tshawb fawb thiab kev kuaj mob. Los ntawm kev nkag siab txog cov txheej txheem tom qab centrifugation thiab nws cov ntawv thov ntau yam, cov kws tshawb fawb txuas ntxiv qhib cov cib fim tshiab rau kev tshawb pom tshiab thiab kev nce qib hauv ntau qhov chaw.
.