se arohanang ka har'a centrifuge

Ke Eng e Arohanyang ka Centrifuge?

Selelekela

Ho centrifugation ke mokhoa o tloaelehileng oa laboratori o kenyeletsang ho arola likarolo tse fapaneng tsa motsoako ho latela bongata ba tsona. Ts'ebetso ena e sebelisa matla a centrifugal ho theha tšimo e matla ea matla a khoheli, e leng se etsang hore likaroloana tse boima li tsamaee ho ea tlase ha tse bobebe li ntse li le holimo. Centrifuge, mochini o etselitsoeng morero ona ka ho khetheha, o bapala karolo ea bohlokoa ho aroleng lintho mafapheng a fapaneng ho kenyeletsoa lipatlisiso tsa bongaka, tsa baeloji le tsa indasteri. Sehloohong sena, re tla teba haholoanyane mohopolong oa centrifugation, ts'ebeliso ea eona, le mekhoa e e lumellang ho arola likarolo tse fapaneng ka katleho.

I. Ho utloisisa Centrifugation

Ho centrifugation ke mokhoa o sebelisang molao-motheo oa ho sedimentation, moo likaroloana kapa lintho tse nang le bongata bo fapaneng li aroloang ka karabelo ea tsona ho matla a centrifugal. Ho fihlela sena, sampole e beoa ka har'a rotor, e potolohang ka lebelo le phahameng. Matla a centrifugal a hlahisoang ke rotor a baka ho sedimentation ha likaroloana tse teteaneng, a li arola ka katleho ho tsoa ho motsoako o mong kaofela.

II. Mefuta ea Li-Centrifuge

1. Li-Ultracentrifuge

Li-centrifuge tsa Ultracentrifuge ke li-centrifuge tse lebelo le phahameng tse ka fihlelang lebelo la ho fihla ho li-revolutions tse 150,000 ka motsotso (rpm). Li sebelisa li-rotor tse nang le likarolo tse khethehileng ho arola likaroloana ho latela boholo le boima ba tsona. Li-centrifuge tsa Ultracentrifuge li fumana lits'ebetso mafapheng a fapaneng a kang baeloji ea limolek'hule, biochemistry le virology.

2. Li-Microcentrifuge

Li-Microcentrifuge ke mefuta e menyenyane ea li-centrifuge tse etselitsoeng ho jala ka potlako li-volume tse nyane. Li sebelisoa haholo lilaboratoring tsa baeloji ea limolek'hule le biochemistry bakeng sa mesebetsi e kang ho hloekisa DNA le protheine, pokello ea li-pellet tsa sele, le ho potoloha ka potlako ha li-tube tsa centrifuge.

III. Litšebeliso tsa Centrifugation

1. Karohano ea Likarolo tsa Mali

E 'ngoe ea lits'ebetso tse tloaelehileng tsa ho arola likarolo tsa mali ke karohano ea likarolo tsa mali. Lilaboratoring tsa bongaka, li-centrifuge li sebelisoa ho arola plasma, lisele tse khubelu tsa mali le lisele tse tšoeu tsa mali ho tsoa lisampoleng tsohle tsa mali. Karohano ena e bohlokoa bakeng sa liteko tsa tlhahlobo, mofuta oa mali le ho lokisa lihlahisoa tse itseng tsa mali, joalo ka li-platelet concentrates.

2. Karohano ea DNA le RNA

Ho centrifugation ho ipaka e le sesebelisoa sa bohlokoa lipatlisisong tsa baeloji ea limolek'hule kaha ho nolofalletsa karohano e sebetsang ea DNA le RNA ho tsoa lisampoleng tse fapaneng. Ka ho centrifuga lisampole tsena ka lebelo le bolelele bo itseng, thepa ea liphatsa tsa lefutso e ka aroloa ho likarolo tse ling tsa sele, e leng se lumellang bo-rasaense ho ithuta le ho sekaseka tatellano ea DNA kapa lipolelo tsa liphatsa tsa lefutso.

3. Karohano ea Litso tsa Sele

Ho baeloji ea sele le theknoloji ea baeloji, ho centrifugation ho sebelisoa ho arola le ho tsepamisa likarolo tsa sele kapa lisele tse felletseng ho tsoa mecheng ea kholo. Ts'ebetso ena e thusa lits'ebetsong tse fapaneng tse kang ho kotula lisele, ho hloekisa protheine, le ho arola li-organelle tsa sele bakeng sa tlhahlobo e eketsehileng.

IV. Mokhoa oa ho Centrifugation

Ho centrifugation ho sebetsa hodima molao-motheo wa matla a centrifugal a sutumelletsang dikarolwana tse teteaneng ho ya mathokong a ka ntle a rotor, e leng se bakang ho senyeha ha mobu. Dikarolo tsa bohlokwa tse amehang tshebetsong ya karohano ke:

1. Liphaephe tsa Rotor le Centrifuge

Rotor e potoloha ho potoloha axis, e hlahisa matla a centrifugal. E tšoara lipeipi tsa centrifuge, tse nang le motsoako o lokelang ho aroloa. Mefuta e fapaneng ea li-rotor le lipeipi e etselitsoe ho amohela boholo bo itseng ba sampole, boholo le matla a centrifugal a hlokahalang.

2. Lebelo le Bolelele

Lebelo le nako ea ho centrifugation li itšetlehile ka mofuta oa sampole le karohano e lakatsehang. Lisebelisoa tse fapaneng, tse kang lisele kapa likokoana-hloko, li ka hloka lebelo le linako tse fapaneng ho fihlela karohano e ntle ka ho fetisisa.

Qetello

Ka bokhutšoanyane, ho centrifugation ke mokhoa oa bohlokoa o sebelisoang ho arola likarolo tse fapaneng tsa motsoako ho latela bongata ba tsona. Li-centrifuge, ebang ke li-centrifuge tse matla kapa li-microcentrifuge, ke lisebelisoa tsa bohlokoa lilaboratoring mafapheng a mangata, tse lumellang bo-rasaense ho fihlella karohano e sebetsang bakeng sa lits'ebetso tse fapaneng. Ho tloha ho arola likarolo tsa mali ho ea ho ithuteng DNA le RNA ho ea ho sebetsana le litso tsa lisele, ho centrifugation ho bapala karolo ea bohlokoa ho ntšetseng pele lipatlisiso tsa saense le tlhahlobo ea mafu. Ka ho utloisisa mekhoa e ka morao ho ho centrifugation le ts'ebeliso ea eona e mengata, bo-rasaense ba tsoela pele ho notlolla menyetla e mecha ea ho sibolla lintho tse ncha le tsoelo-pele mafapheng a fapaneng.