he aha te mea ka wehea i roto i te centrifuge

He aha ngā mea e wehea ana i roto i te centrifuge?

Kupu Whakataki

He tikanga taiwhanga noa te centrifugation e wehewehe ana i ngā wāhanga rerekē o te ranunga i runga i tō rātou mātotoru. Ka whakamahia e tēnei tukanga te kaha centrifugation hei waihanga i tētahi papa kaha, e meinga ai ngā matūriki taumaha kia neke ki raro, ko ngā mea māmā ake kei runga tonu. Ko te centrifugation, he mīhini i hangaia mō tēnei kaupapa, he mahi nui tāna ki te wehewehe i ngā matū i roto i ngā mara rerekē tae atu ki te rangahau hauora, koiora, me te ahumahi. I roto i tēnei tuhinga, ka ruku hohonu tātou ki te ariā o te centrifugation, ōna tono, me ngā tikanga e taea ai e ia te wehewehe i ngā wāhanga rerekē kia whai hua.

I. Te Mārama ki te Whakapūngao

Ko te centrifugation he tikanga e whakamahi ana i te kaupapa o te whakapūpū, e wehea ai ngā matūriki, ngā matū rānei o ngā matotoru rerekē mā te urupare ki te kaha o te centrifugation. Hei whakatutuki i tēnei, ka whakanohoia he tauira ki roto i te rotor, e hurihuri ana i ngā tere teitei. Mā te kaha centrifugation i hangaia e te rotor ka whakapūpū ngā matūriki kiato ake, ka wehea ai rātou i te toenga o te ranunga.

II. Ngā Momo o ngā Pūwhakapūngao

1. Ngā Pūwhakawhitiwhiti Tino-rahi

He tere rawa ngā pūwhakawhitiwhiti ultra-centrifuge, ā, ka taea e rātou te eke ki te tere tae atu ki te 150,000 hurihanga ia meneti (rpm). Ka whakamahia e rātou he rotor me ngā wāhanga motuhake hei wehewehe i ngā matūriki i runga i ō rātou rahi me te mātotoru. Ka kitea ngā whakamahinga o ngā pūwhakawhitiwhiti ultra-centrifuge i roto i ngā mara maha pērā i te koiora ngota, te matū koiora, me te virology.

2. Ngā Pūwhakawhitiwhiti Iti

He momo iti ake ngā mīhini whakapūtāhui iti i hangaia mō te whakapūtāhui tere i ngā rahinga iti. He whānuitia te whakamahinga i roto i ngā taiwhanga koiora ngota me te matū koiora mō ngā mahi pēnei i te purenga DNA me te pūmua, te kohikohi i ngā pirepire pūtau, me te huri tere i ngā ngongo whakapūtāhui.

III. Ngā Whakamahinga o te Whakapūtanga

1. Te Wehewehenga o ngā Wāhanga Toto

Ko tētahi o ngā whakamahinga tino noa o te centrifugation ko te wehenga o ngā wāhanga toto. I roto i ngā taiwhanga hauora, ka whakamahia ngā centrifuge hei wehe i te plasma, ngā toto whero, me ngā toto mā mai i ngā tauira toto katoa. He mea nui tēnei wehenga mō ngā whakamātautau tātaritanga, te momo toto, me te whakarite i ngā hua toto motuhake, pērā i ngā kukū pereti toto.

2. Te wehenga o te DNA me te RNA

He taputapu tino nui te whakamahi i te pūwero i roto i te rangahau koiora ngota, nā te mea ka taea te wehewehe pai i te DNA me te RNA mai i ngā tauira rerekē. Mā te whakamahi i ēnei tauira i ngā tere me ngā roanga motuhake, ka taea te wehewehe i ngā rauemi ira mai i ētahi atu wāhanga pūtau, ā, ka taea e ngā kaipūtaiao te ako me te tātari i ngā raupapatanga DNA, i ngā whakaaturanga ira rānei.

3. Te Wehewehenga o ngā Ahurea Pūtau

I roto i te koiora pūtau me te hangarau koiora, ka whakamahia te centrifugation hei wehe me te whakaemi i ngā wāhanga pūtau, i ngā pūtau katoa rānei mai i ngā rauemi tipu. Ka āwhina tēnei tukanga i ngā momo tono pēnei i te hauhake pūtau, te purenga pūmua, me te wehenga o ngā whekau pūtau mō ētahi atu tātaritanga.

IV. Tikanga Whakapūngao

Ko te mahi a te pūwero i runga i te kaupapa o te kaha pūwero e pana ana i ngā matūriki matotoru ki ngā taha o waho o te rotor, ka puta ai te whakapūpū. Ko ngā wāhanga matua e uru ana ki te tukanga wehenga ko:

1. Ngā Ngongo Rotor me te Centrifuge

Ka huri te rotor i tētahi tuaka, ka whakaputa i te kaha whakatere. Ka pupuri i ngā ngongo whakatere, kei roto nei te ranunga hei wehe. He rerekē ngā momo rotor me ngā ngongo kua hangaia hei whakauru i ngā rahinga tauira, ngā rahi, me ngā kaha whakatere e hiahiatia ana.

2. Tere me te Roanga

Ko te tere me te roa o te centrifugation e whakawhirinaki ana ki te āhua o te tauira me te wehenga e hiahiatia ana. Tērā pea ka hiahiatia he tere me he roa rerekē mō ngā rauemi rerekē, pērā i ngā pūtau, i ngā moroiti rānei, kia tutuki ai te wehenga tino pai.

Whakamutunga

Hei whakarāpopototanga, he tikanga nui te centrifugation hei wehewehe i ngā wāhanga rerekē o te ranunga i runga i tō rātou mātotoru. He taputapu nui ngā centrifugation, ahakoa he ultracentrifuge, he microcentrifuge rānei, i roto i ngā taiwhanga puta noa i ngā mara maha, e āhei ai ngā kaipūtaiao ki te whakatutuki i te wehenga whai hua mō ngā tono rerekē. Mai i te wehewehe i ngā wāhanga toto ki te ako i te DNA me te RNA ki te tukatuka i ngā ahurea pūtau, he mahi nui te centrifugation ki te whakatairanga i te rangahau pūtaiao me te tātaritanga. Mā te mārama ki ngā tikanga kei muri i te centrifugation me ōna tono maha, ka haere tonu ngā kaipūtaiao ki te whakatuwhera i ngā whai wāhitanga hou mō ngā kitenga hou me ngā whanaketanga i roto i ngā mara rerekē.