Kodi centrifuge imalola chiyani scintist kulekanitsa

Kodi Centrifuge Imathandiza Wasayansi Kulekanitsa Chiyani?

Chiyambi:

Sentirifuge ndi chida chofunikira kwambiri m'gulu la asayansi, chomwe chimalola ofufuza kusiyanitsa zinthu zosiyanasiyana kutengera kuchuluka ndi kukula kwake. Chida champhamvu ichi chimagwiritsa ntchito mphamvu ya sentirifuge kuti chigawane, zomwe zimathandiza asayansi kuphunzira ndikufufuza zigawo zosiyanasiyana mkati mwa chitsanzo. Kuyambira kulekanitsa maselo amagazi mpaka kuchotsa DNA, sentirifuge imagwira ntchito yofunika kwambiri m'magawo ambiri asayansi. M'nkhaniyi, tifufuza momwe sentirifuge imagwirira ntchito komanso momwe imathandizira asayansi kulekanitsa zinthu moyenera.

I. Mfundo ya Centrifugation:

Kugawa zinthu pogwiritsa ntchito njira yo ...

II. Kulekanitsa Zigawo za Magazi:

Chimodzi mwa ntchito zomwe zimagwiritsidwa ntchito kwambiri ndi centrifuge ndikulekanitsa zigawo za magazi. Pogwiritsa ntchito chubu chapadera chosonkhanitsira magazi chokhala ndi anticoagulant, chitsanzo cha magazi chikhoza kuyikidwa mu centrifuge. Chifukwa cha kuchulukana kosiyanasiyana kwa zigawo zake, magazi amagawikana m'magawo atatu osiyana - plasma pamwamba, wosanjikiza woonda wa maselo oyera amagazi ndi ma platelet, ndi wosanjikiza wa maselo ofiira amagazi pansi. Akatswiri azachipatala amatha kupeza zigawo zinazake kuti ayesedwe matenda kapena njira zachipatala, monga kupatula plasma kuti ifufuze kapangidwe ka mankhwala kapena kupeza plasma yolemera ma platelet kuti igwiritsidwe ntchito pochiza matenda obadwanso.

III. Kuchotsa DNA:

Sentirifuge imagwiranso ntchito yofunika kwambiri pakuchotsa DNA, njira yofunika kwambiri mu biology ya mamolekyulu ndi kafukufuku wa majini. Pogwiritsa ntchito sentirifuge, asayansi amatha kulekanitsa DNA ndi zigawo zina za maselo, monga mapuloteni ndi mafuta. Poyamba kuchotsa DNA, zitsanzo zamoyo (monga maselo kapena minofu) zimachiritsidwa ndi ma reagents osiyanasiyana kuti ziswe nembanemba ya selo ndikutulutsa DNA. Kenako chisakanizocho chimayikidwa pakati, zomwe zimapangitsa kuti zinyalala zolemera za maselo ndi mapuloteni zilekanitsidwe ndikupanga pellet pansi pomwe DNA imakhalabe mu supernatant. Mwa kuchotsa supernatant mosamala, asayansi amatha kupatula DNA kuti afufuzenso, monga kutsata kapena kuyika cloning.

IV. Kupatula Zigawo Zam'maselo:

Mothandizidwa ndi ma centrifuge apamwamba, asayansi amatha kusiyanitsa bwino zigawo za subcellular kuti aphunzire ntchito ndi makhalidwe awo. Mwachitsanzo, mitochondria, ma organelles opanga mphamvu mkati mwa maselo, amatha kulekanitsidwa pogwiritsa ntchito centrifuge kutengera kukula ndi kuchuluka kwawo. Pogwiritsa ntchito njira zingapo zoyendetsera centrifuge pa liwiro losiyanasiyana, ofufuza amatha kupeza zigawo za mitochondria zoyeretsedwa kuti ziwunike mozama ndikuphunzira ntchito zawo pa kagayidwe ka maselo ndi chitukuko cha matenda. Njira zofananazi zingagwiritsidwe ntchito posiyanitsa zigawo zina za subcellular, monga nuclei kapena cellular membranes, zomwe zimathandiza kufufuza mokwanira kapangidwe kake ndi ntchito zake.

V. Kulekanitsa Mapuloteni a Lipo:

Ma lipoprotein ndi mankhwala ovuta omwe amapangidwa ndi mafuta ndi mapuloteni. Amagwira ntchito yofunika kwambiri ponyamula mafuta, cholesterol, ndi mafuta ena m'magazi. Kutsegula magazi kumagwiritsidwa ntchito kwambiri m'ma laboratories azachipatala kuti alekanitse lipoproteins kuti azindikire matenda. Mwa kupotoza chitsanzo cha magazi pa liwiro lalikulu, lipoproteins zimagawidwa m'magawo osiyanasiyana a density, omwe amatha kufufuzidwa kuti adziwe lipids. Ma profiles awa amapereka chidziwitso chofunikira pa thanzi la mtima wa munthu, chifukwa milingo ya lipoprotein yosazolowereka nthawi zambiri imagwirizanitsidwa ndi matenda monga hyperlipidemia ndi atherosclerosis.

Mapeto:

Sentirifuge ndi chida cha sayansi chogwiritsidwa ntchito m'njira zosiyanasiyana chomwe chimalola ofufuza kulekanitsa zinthu, zigawo, ndi tinthu tating'onoting'ono molondola komanso moyenera. Kaya ndi kulekanitsa zigawo za magazi, kuchotsa DNA, kapena kuphunzira za ma organelles ang'onoang'ono a maselo, sentirifuge imagwira ntchito yofunika kwambiri pakupititsa patsogolo chidziwitso cha sayansi ndi kumvetsetsa. Mwa kugwiritsa ntchito mfundo za sentirifuge, asayansi amatha kufufuza zovuta za machitidwe osiyanasiyana ndikuvumbula zinsinsi zobisika mkati mwa zitsanzo zambiri.