He aha ka mea e hiki ai i ka centrifuge ke hoʻokaʻawale i kahi scintist
He aha ka mea e hiki ai i ka Centrifuge ke hoʻokaʻawale i kahi ʻepekema?
Hoʻolauna:
He mea hana koʻikoʻi ka centrifuge i loko o ke kaiāulu ʻepekema, e ʻae ana i nā mea noiʻi e hoʻokaʻawale i nā mea like ʻole ma muli o ko lākou nui a me ka nui. Hoʻohana kēia mea hana ikaika i ka ikaika centrifugal e hoʻokaʻawale ai, e hiki ai i nā ʻepekema ke aʻo a noiʻi i nā ʻāpana like ʻole i loko o kahi laʻana. Mai ka hoʻokaʻawale ʻana o nā hunaola koko a hiki i ka unuhi ʻana o ka DNA, he kuleana koʻikoʻi ka centrifuge i nā ʻano aʻo ʻepekema he nui. Ma kēia ʻatikala, e ʻimi mākou i nā hiki o kahi centrifuge a pehea e hiki ai i nā ʻepekema ke hoʻokaʻawale pono i nā mea.
I. Ke Kumumanaʻo o ka Centrifugation:
ʻO ke centrifugation kahi ʻano hana e hoʻohana ana i ke kumumanaʻo o ka sedimentation e hoʻokaʻawale i nā mea i loko o kahi hui ʻana ma muli o ko lākou nui. Ke hoʻohuli ʻia ka laʻana i ka wikiwiki kiʻekiʻe i loko o ka centrifuge, ʻike nā ʻāpana i kahi ikaika centrifugal, e hoʻoneʻe ana iā lākou mai ke kikowaena. A laila noho nā ʻāpana i loko o nā papa like ʻole, e ʻae ana i ka hoʻokaʻawale maʻalahi.
II. Ke hoʻokaʻawale ʻana i nā ʻāpana koko:
ʻO kekahi o nā hoʻohana maʻamau o ka centrifuge ʻo ia ka hoʻokaʻawale ʻana i nā ʻāpana koko. Ma ka hoʻohana ʻana i kahi paipu hōʻiliʻili koko kūikawā me kahi anticoagulant, hiki ke waiho ʻia ka hāpana koko i loko o ka centrifuge. Ma muli o nā ʻano mānoanoa like ʻole o kona mau ʻāpana, e hoʻokaʻawale ke koko i ʻekolu mau papa like ʻole - plasma ma luna, kahi papa lahilahi o nā keʻokeʻo koko a me nā platelets, a me kahi papa o nā keʻokeʻo koko ʻulaʻula ma lalo. A laila hiki i nā loea lapaʻau ke loaʻa nā ʻāpana kikoʻī no nā hoʻokolohua diagnostic a i ʻole nā kaʻina hana lapaʻau, e like me ka hoʻokaʻawale ʻana i ka plasma no ka nānā ʻana i nā ʻano kemika a i ʻole ka loaʻa ʻana o ka plasma momona platelet no nā lāʻau lapaʻau regenerative.
III. Ka Hoʻokaʻawale ʻana i ka DNA:
He kuleana koʻikoʻi nō hoʻi ka centrifuge i ka unuhi ʻana o ka DNA, kahi hana koʻikoʻi i ka biology molecular a me ka noiʻi genetic. Ma ka hoʻohana ʻana i ka centrifugation, hiki i nā kānaka ʻepekema ke hoʻokaʻawale i ka DNA mai nā ʻāpana cellular ʻē aʻe, e like me nā protein a me nā lipids. I nā ʻanuʻu mua o ka unuhi ʻana o ka DNA, mālama ʻia nā laʻana biological (e laʻa, nā cell a i ʻole nā ʻiʻo) me nā reagents like ʻole e wāwahi i ka membrane cell a hoʻokuʻu i ka DNA. A laila centrifuged ka hui ʻana, e hoʻokaʻawale ana i nā ʻōpala cell kaumaha a me nā protein a hana i kahi pellet ma lalo ʻoiai e noho ana ka DNA i loko o ka supernatant. Ma ka wehe pono ʻana i ka supernatant, hiki i nā kānaka ʻepekema ke hoʻokaʻawale i ka DNA no ka nānā hou aku, e like me ka sequencing a i ʻole cloning.
IV. Ke hoʻokaʻawale ʻana i nā ʻāpana subcellular:
Me ke kōkua o nā centrifuges holomua, hiki i nā kānaka ʻepekema ke hoʻokaʻawale pono i nā ʻāpana subcellular e aʻo i kā lākou mau hana a me nā ʻano. No ka laʻana, hiki ke hoʻokaʻawale ʻia ka mitochondria, nā organelles e hana ana i ka ikehu i loko o nā cell, me ka hoʻohana ʻana i ka centrifugation ma muli o ko lākou nui a me ka nui. Ma ka hoʻohana ʻana i kahi moʻo o nā ʻanuʻu centrifugation ma nā wikiwiki like ʻole, hiki i nā mea noiʻi ke loaʻa nā ʻāpana mitochondria i hoʻomaʻemaʻe ʻia no ka nānā hohonu a aʻo i kā lākou kuleana i ka metabolism cellular a me ka ulu ʻana o nā maʻi. Hiki ke hoʻopili ʻia nā ʻano hana like e hoʻokaʻawale i nā ʻāpana subcellular ʻē aʻe, e like me nā nuclei a i ʻole nā membranes cellular, e hiki ai ke noiʻi piha i ko lākou ʻano a me nā hana.
V. Ka hoʻokaʻawale ʻana o nā Lipoproteins:
He mau hui paʻakikī nā lipoproteins i haku ʻia me nā lipids a me nā protein. He kuleana koʻikoʻi ko lākou i ka lawe ʻana i nā momona, cholesterol, a me nā lipids ʻē aʻe i loko o ke koko. Hoʻohana nui ʻia ka centrifugation i nā keʻena hoʻokolohua lapaʻau e hoʻokaʻawale i nā lipoproteins no nā kumu diagnostic. Ma ka milo ʻana i kahi hāpana koko i nā wikiwiki kiʻekiʻe, hoʻolaha ʻia nā lipoproteins i nā ʻāpana density like ʻole, a laila hiki ke kālailai ʻia e hoʻoholo i nā ʻikepili lipid. Hāʻawi kēia mau ʻikepili i nā ʻike waiwai i ke olakino cardiovascular o ke kanaka, ʻoiai ʻo nā pae lipoprotein maʻamau ʻole e pili pinepine ʻia me nā kūlana e like me ka hyperlipidemia a me ka atherosclerosis.
Hopena:
He mea hana ʻepekema maʻalahi ka centrifuge e hiki ai i nā mea noiʻi ke hoʻokaʻawale i nā mea, nā ʻāpana, a me nā ʻāpana me ka pololei a me ka pono. Inā paha he hoʻokaʻawale ʻana i nā ʻāpana koko, ka unuhi ʻana i ka DNA, a i ʻole ke aʻo ʻana i nā organelles subcellular, he kuleana koʻikoʻi ka centrifuge i ka hoʻolaha ʻana i ka ʻike ʻepekema a me ka hoʻomaopopo ʻana. Ma ka hoʻohana ʻana i nā kumumanaʻo o ka centrifugation, hiki i nā kānaka ʻepekema ke ʻimi i nā paʻakikī o nā ʻōnaehana like ʻole a wehe i nā mea pohihihi i hūnā ʻia i loko o nā laʻana helu ʻole.
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