momwe mungalekanitsire maselo ofiira a magazi kuchokera ku centrifuge yamagazi yonse

Njira Zatsopano Zolekanitsira Maselo Ofiira a Magazi ndi Magazi Athunthu

Chiyambi:

Magazi ndi madzi ovuta komanso ofunikira omwe ali ndi zigawo zosiyanasiyana, kuphatikizapo maselo ofiira a magazi (RBC), maselo oyera a magazi, plasma, ndi ma platelet. Mu ntchito zina zachipatala ndi kafukufuku, zimakhala zofunikira kulekanitsa zigawo zinazake za magazi, monga RBC, ndi magazi onse. Njira imodzi yothandiza kwambiri yopezera kulekanitsa kumeneku ndi kugwiritsa ntchito centrifuge. M'nkhaniyi, tifufuza njira zosiyanasiyana zatsopano zomwe zilipo zolekanitsira RBC ndi magazi onse pogwiritsa ntchito centrifuge.

I. Kumvetsetsa Njira Yogwiritsira Ntchito Centrifugation:

Kupondaponda ndi njira yomwe imagwiritsa ntchito mfundo za kupondaponda ndi mphamvu ya kupondaponda kuti ilekanitse tinthu tating'onoting'ono tosiyanasiyana. Mwa kupotoza chitsanzo pa liwiro lalikulu, tinthu tating'onoting'ono, monga ma RBC, timapondaponda pansi, zomwe zimapangitsa kuti tisiyane ndi zinthu zina.

II. Kugwiritsa Ntchito Differential Density Gradient Centrifugation:

Njira imodzi yomwe imagwiritsidwa ntchito kwambiri polekanitsa maselo ofiira a m'magazi ndi magazi athunthu ndi differential density gradient centrifugation. Njirayi imagwiritsa ntchito kusiyana kwa density pakati pa zigawo zosiyanasiyana za magazi. Mwa kuyika chitsanzo pamwamba pa density gradient medium, zigawozo zimasiyana kutengera kuchuluka kwawo.

III. Kugwiritsa Ntchito Ficoll-Paque Density Gradient Centrifugation:

Ficoll-Paque ndi reagent yomwe imagwiritsidwa ntchito kwambiri poyezera kuchuluka kwa magazi chifukwa cha mphamvu yake yopangira ma gradient okhazikika. Njira imeneyi imaphatikizapo kuyika mosamala kuchuluka kwa magazi athunthu pa Ficoll-Paque, kutsatiridwa ndi centrifugation pa liwiro lolamulidwa komanso nthawi yake. Zotsatira zake, ma RBC amapanga gulu losiyana pansi pa plasma, zomwe zimathandiza kuti pakhale kusiyana kosavuta.

IV. Kukonza Ma Centrifugation Parameters kuti Pakhale Kulekanitsa Koyenera:

Kuti tikwaniritse kulekanitsa bwino maselo ofiira a m'magazi kuchokera ku magazi athunthu, kukonza bwino magawo a centrifugation ndikofunikira kwambiri. Zinthu monga liwiro, nthawi, ndi kutentha zimathandiza kwambiri pakudziwa momwe kulekanitsa kumagwirira ntchito. Mwa kusintha zinthuzi, ofufuza amatha kuwonjezera kuchuluka ndi chiyero cha maselo ofiira a m'magazi omwe amapezeka kuchokera ku magazi athunthu.

V. Kubwera kwa Microfluidics ndi Lab-on-a-Chip Technologies:

M'zaka zaposachedwapa, ma microfluidics ndi ukadaulo wa lab-on-a-chip zakhala njira zina zabwino m'malo mwa njira zachikhalidwe zoyezera magazi. Njira zamakonozi zimaphatikiza zida zazing'ono zomwe zimayendetsa magazi ochepa, pogwiritsa ntchito mfundo monga kuyenda kwa hydrodynamic, mphamvu zamawu, ndi njira zosefera kuti zilekanitse ma RBC ndi magazi athunthu. Njira zazing'ono izi zimapereka zabwino zingapo, kuphatikiza kuchepa kwa kuchuluka kwa zitsanzo, kupititsa patsogolo mphamvu zamagetsi, komanso kuwongolera bwino magawo olekanitsa.

VI. Kusankha kwa Ma RBC a Microfluidic Kutengera ndi Kapangidwe ka Biophysical:

Mapulatifomu a microfluidic amalola kulekanitsa molondola ma RBC pogwiritsa ntchito mphamvu zawo za biophysical. Mwa kupanga njira zazing'ono zokhala ndi geometries yapadera ndikugwiritsa ntchito njira monga kulekanitsa komwe kumayambitsa kusokonekera, kuyang'ana kwa inertial, kapena dielectrophoresis, ndizotheka kusanja ma RBC kutengera mawonekedwe monga kukula, mawonekedwe, ndi kuuma. Zipangizo zazing'ono izi zimapereka chidziwitso chapamwamba pakulekanitsa ma RBC ndikuthandizira kugwiritsa ntchito njira zoyambira pozindikira matenda, kuyika magazi, ndi kafukufuku.

Mapeto:

Kulekanitsa maselo ofiira a m'magazi ndi magazi athunthu ndi gawo lofunika kwambiri pa ntchito zambiri zachipatala ndi kafukufuku. Njira zoyezera magazi, monga differential density gradient centrifugation pogwiritsa ntchito Ficoll-Paque, zagwiritsidwa ntchito kwambiri pa izi. Komabe, kupita patsogolo kwaposachedwa mu microfluidics ndi ukadaulo wa lab-on-a-chip kumapereka njira zina zosangalatsa zoyezera magazi a m'magazi, zomwe zimapangitsa kuti pakhale kugwira ntchito bwino, kuchepetsa kuchuluka kwa zitsanzo, komanso kuwongolera bwino njira yoyezera magazi. Pamene kafukufuku m'munda uno akupitilizabe kupita patsogolo, njira zatsopanozi zili ndi kuthekera kwakukulu kosinthira njira zoyezera magazi ndikukweza magawo osiyanasiyana a kafukufuku wazachipatala ndi sayansi.