Me pēhea te wehewehe i ngā toto whero mai i te centrifuge toto katoa
Ngā Tikanga Auaha hei Wehe i ngā Pūtau Toto Whero mai i te Toto Katoa
Kupu Whakataki:
He wai matatini, he wai ora hoki te toto, kei roto ko ngā momo wāhanga, tae atu ki ngā toto whero (RBC), ngā toto mā, te parāuma, me ngā pereti toto. I roto i ētahi mahi hauora me te rangahau, me wehe ngā wāhanga toto motuhake, pērā i ngā toto whero, mai i te toto katoa. Ko tētahi o ngā tikanga tino whai hua mō te whakatutuki i tēnei wehenga ko te whakamahi i te centrifuge. I roto i tēnei tuhinga, ka tūhuratia e mātou ngā tikanga auaha rerekē e wātea ana hei wehe i ngā toto whero mai i te toto katoa mā te whakamahi i te centrifugation.
I. Te Mārama ki te Tukanga Whakapūngao:
Ko te whakapūpū he tikanga e whakamahi ana i ngā mātāpono o te whakapūpū me te kaha whakapūpū hei wehewehe i ngā matūriki o ngā matotoru rerekē. Mā te hurihuri i tētahi tauira i ngā tere teitei, ka whakapūpū ngā matūriki matotoru, pērā i ngā toto whero, ki raro, ka taea te wehe i ngā wāhanga kē atu.
II. Te Whakamahi i te Whakapūtanga Rerekētanga o te Matotoru:
Ko tētahi o ngā tikanga e whakamahia whānuitia ana hei wehewehe i ngā toto whero mai i te toto katoa ko te centrifugation rerekētanga o te mātotorutanga. Ka whakamahia e tēnei tikanga ngā rerekētanga mātotorutanga i waenga i ngā wāhanga rerekē o te toto. Mā te whakatakoto i tētahi tauira ki runga i tētahi reo mātotorutanga, ka wehea ngā wāhanga i runga i ō rātou mātotorutanga motuhake.
III. Te Whakamahi i te Whakapūtanga Taumaha Ficoll-Paque:
He matū whakamahi whānuitia te Ficoll-Paque mō te whakapūtanga i te pikinga mātotoru nā tōna kaha ki te hanga i ngā pikinga pumau. Ko tēnei tikanga he āta whakatakoto i te nui o te toto katoa e hiahiatia ana ki runga i te Ficoll-Paque, ā, muri iho ka whakapūtanga i te tere me te roa e whakahaeretia ana. Nō reira, ka hangaia e ngā toto whero he whitiki motuhake i raro i te parāuma, e ngāwari ai te wehenga.
IV. Te Arotau i ngā Tawhā Whakapūtanga mō te Wehenga Pai Ake:
Hei whakatutuki i te wehenga whai hua o ngā toto whero mai i te toto katoa, he mea nui te arotau i ngā tawhā centrifugation. Ko ngā āhuatanga pēnei i te tere, te roa, me te pāmahana he mea nui ki te whakatau i te whai huatanga o te wehenga. Mā te whakatikatika i ēnei taurangi, ka taea e ngā kairangahau te whakarei ake i te hua me te parakore o ngā toto whero i whiwhihia mai i te toto katoa.
V. Te Haerenga Mai o ngā Hangarau Maikorowai me ngā Hangarau Lab-on-a-Chip:
I ngā tau tata nei, kua puta ake ngā hangarau moroiti me ngā hangarau taiwhanga-i-runga-i-te-maramara hei huarahi whai hua ki ngā tikanga whakapūmau tikanga. Ka whakauruhia e ēnei huarahi hou ngā taputapu iti e whakahaere ana i ngā rahinga toto iti, e whakamahi ana i ngā mātāpono pēnei i te rere wai, ngā kaha oro, me ngā tikanga tātari hei wehe i ngā toto whero mai i te toto katoa. He maha ngā painga o ēnei tikanga moroiti, tae atu ki te whakaiti i ngā whakaritenga rahinga tauira, te whakapai ake i te rere o te toto, me te whakahaere pai ake i ngā tawhā wehenga.
VI. Te Whakarōpūtanga Maikoro-wai o ngā RBC i runga i ngā Āhuatanga Koiora-tinana:
Mā ngā tūāpapa moroiti-wai ka taea te wehewehe tika i ngā toto whero mā te whakamahi i ō rātou āhuatanga koiora-tinana. Mā te hoahoa i ngā awa moroiti me ngā āhuahanga motuhake me te whakamahi i ngā tikanga pēnei i te wehenga i puta mai i te whakarerekētanga, te arotahi inertial, te dielectrophoresis rānei, ka taea te whakarōpū i ngā toto whero i runga i ngā āhuatanga pēnei i te rahi, te āhua, me te pakari. Ka tukuna e ēnei taputapu moroiti-wai he tino motuhake ki te wehe i ngā toto whero, ā, ka taea ai ngā tono o muri mai i roto i te taatai, te rongoā whakawhiti toto, me te rangahau.
Whakatau:
He taahiraa nui te wehewehe i ngā toto whero mai i te toto katoa i roto i ngā mahi hauora me ngā mahi rangahau maha. Kua whānuitia te whakamahinga o ngā tikanga centrifugation, pērā i te centrifugation gradient differential density ma te whakamahi i te Ficoll-Paque, mō tēnei kaupapa. Heoi, ko ngā whanaketanga hou o te microfluidics me ngā hangarau taiwhanga-i-runga-i-te-maramara he huarahi whakahihiri mō te wehenga toto whero, e whakarato ana i te pai ake o te mahi, te whakaiti i ngā whakaritenga rahinga tauira, me te whakahaere pai ake i te tukanga wehenga. I te haere tonu o te rangahau i tēnei mara, he nui te kaha o ēnei tikanga auaha ki te huri i ngā tikanga wehenga toto me te whakapai ake i ngā momo mara rangahau hauora me te pūtaiao.
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