nzira yekuparadzanisa masero matsvuku eropa kubva kumasero ese eropa

Maitiro Ekuvandudza Ekuparadzanisa Masero Matsvuku Eropa Kubva kuRopa Rose

Nhanganyaya:

Ropa imvura yakaoma uye inokosha ine zvikamu zvakasiyana-siyana, zvinosanganisira masero matsvuku eropa (RBCs), masero machena eropa, plasma, uye maplatelet. Mune mamwe mashandisirwo ekurapa nekutsvaga, zvinova zvakakosha kuparadzanisa zvikamu zveropa, zvakaita seRBCs, kubva muropa rose. Imwe yenzira dzinoshanda kwazvo dzekuwana kupatsanurwa uku ndeyekushandisa centrifuge. Muchinyorwa chino, tichaongorora nzira dzakasiyana dzekuvandudza dziripo dzekuparadzanisa RBCs kubva kuropa rose tichishandisa centrifugation.

I. Kunzwisisa Maitiro eCentrifugation:

Kupinza simbi mumhepo (centrifugation) inzira inoshandisa misimboti yekuparadza simbi uye simba rekuparadzanisa simbi kuti iparadzanise simbi dzine huwandu hwakasiyana. Nekutenderedza sample nekumhanya kwakanyanya, simbi dzakasimba, seRBCs, dzinoisa simbi pasi, zvichiita kuti dziparadzane nedzimwe nhengo.

II. Kushandisa Differential Density Gradient Centrifugation:

Imwe yenzira dzinonyanya kushandiswa kupatsanura maRBC kubva kuropa rose ndeye differential density gradient centrifugation. Nzira iyi inoshandisa musiyano we density pakati pezvikamu zvakasiyana zveropa. Nekuisa sampuro pamusoro pe density gradient medium, zvikamu zvinopatsanurwa zvichienderana nehuremu hwazvo.

III. Kushandisa Ficoll-Paque Density Gradient Centrifugation:

Ficoll-Paque imushonga unoshandiswa zvakanyanya pakubvisa density gradient centrifugation nekuda kwekugona kwayo kugadzira ma gradient akagadzikana. Iyi nzira inosanganisira kuisa vhoriyamu yeropa rose paFicoll-Paque nekungwarira, zvichiteverwa ne centrifugation nekumhanya kwakarongwa uye kwenguva yakareba. Nekuda kweizvozvo, maRBC anoumba band rakasiyana pasi peplasma, zvichiita kuti zvive nyore kupatsanurana.

IV. Kugadzirisa Maparamita eCentrifugation ekuvandudza Kuparadzaniswa:

Kuti pave nekupatsanurwa kwakanaka kwemaRBC kubva muropa rose, kugadzirisa ma centrifugation parameters kwakakosha. Zvinhu zvakaita sekumhanya, nguva, uye tembiricha zvinoita basa guru mukuona kushanda kwekupatsanurwa. Nekugadzirisa izvi, vaongorori vanogona kuwedzera goho uye kuchena kwemaRBC anowanikwa muropa rose.

V. Kuuya kweMicrofluidics neLab-on-a-Chip Technologies:

Mumakore achangopfuura, matekinoroji e microfluidics uye lab-on-a-chip ave achionekwa senzira dzinovimbisa dzekushandisa nzira dzemazuva ano dze centrifugation. Nzira idzi dzemazuva ano dzinobatanidza michina midiki inoshandisa ropa diki, ichishandisa misimboti yakaita se hydrodynamic flow, acoustic forces, uye filtration mechanisms kuti iparadzanise maRBC kubva kuropa rose. Aya matekinoroji e microscale anopa mabhenefiti akati wandei, anosanganisira kudzikiswa kwehuwandu hwesampuro, kuvandudzwa kwe throughput, uye kuvandudzwa kwekutonga pamusoro pe parameters dzekuparadzanisa.

VI. Kurongeka kwemaRBC mu microfluidic Zvichienderana neHunhu hweBiophysical:

Mapuratifomu e microfluidic anobvumira kupatsanurwa kwakanyatsojeka kwemasero eropa nekushandisa hunhu hwawo hwe biophysical. Nekugadzira ma microchannels ane geometries chaiyo uye kushandisa matekiniki akadai sekupatsanurwa kunokonzerwa ne deformability-induced, inertial focus, kana dielectrophoresis, zvinokwanisika kupatsanura ma RBC zvichienderana nehunhu hwakadai sehukuru, chimiro, uye kuomarara. Midziyo iyi ye microfluidic inopa hunyanzvi hwakanyanya mukuparadzanisa ma RBC uye inobvumira kushandiswa kwemashure mukuongorora, kurapa kweropa, uye kutsvagisa.

Mhedziso:

Kuparadzanisa maRBC kubva kuropa rose idanho rakakosha mune zvakawanda zvinoshandiswa pakurapa nekutsvaga. Matekiniki e centrifugation, akadai se differential density gradient centrifugation uchishandisa Ficoll-Paque, akashandiswa zvakanyanya pachinangwa ichi. Zvisinei, kufambira mberi kwazvino mu microfluidics uye lab-on-a-chip tekinoroji zvinopa dzimwe nzira dzinonakidza dzekuparadzanisa maRBC, zvichipa kushanda kwakawedzerwa, kuderedzwa kwehuwandu hwesampuli, uye kudzora kwakawedzerwa maitiro ekuparadzanisa. Sezvo tsvakiridzo mundima iyi ichiramba ichifambira mberi, matekiniki aya matsva ane mukana mukuru wekuchinja nzira dzekuparadzanisa ropa uye kuvandudza nzvimbo dzakasiyana dzekutsvaga kwekurapa nesainzi.