mokhoa oa ho arola lisele tse khubelu tsa mali ho centrifuge ea mali kaofela

Mekhoa e Mecha ea ho Arohanya Lisele tse Khubelu tsa Mali ho Mali a Feletseng

Selelekela:

Mali ke mokelikeli o rarahaneng le oa bohlokoa o nang le likarolo tse fapaneng, ho kenyeletsoa lisele tse khubelu tsa mali (RBC), lisele tse tšoeu tsa mali, plasma le li-platelet. Litšebelisong tse itseng tsa bongaka le tsa lipatlisiso, hoa hlokahala ho arola likarolo tse itseng tsa mali, joalo ka li-RBC, maling 'ohle. E 'ngoe ea mekhoa e sebetsang ka ho fetisisa ea ho fihlela karohano ena ke ka ho sebelisa centrifuge. Sehloohong sena, re tla hlahloba mekhoa e fapaneng e mecha e fumanehang ea ho arola li-RBC maling 'ohle ka ho sebelisa centrifuge.

I. Ho utloisisa Mokhoa oa ho Centrifugation:

Ho Centrifugation ke mokhoa o sebedisang melaomotheo ya ho sedimentation le matla a centrifugal ho arola dikarolwana tse nang le bongata bo fapaneng. Ka ho potoloha sampole ka lebelo le phahameng, dikarolwana tse teteaneng, jwalo ka di-RBC, di sediment ho ya tlase, e leng se dumellang karohano ho dikarolwana tse ding.

II. Ho Sebelisa Differential Density Gradient Centrifugation:

E 'ngoe ea mekhoa e sebelisoang haholo ho arola lisele tse khubelu tsa mali maling kaofela ke ho arola lisele tse khubelu tsa mali ka tekanyo e fapaneng. Mokhoa ona o sebelisa liphapang tsa khatello ea mali lipakeng tsa likarolo tse fapaneng tsa mali. Ka ho beha sampole holim'a sebaka sa khatello ea mali, likarolo lia arohana ho latela bongata ba tsona bo itseng.

III. Ho Sebelisa Ficoll-Paque Density Gradient Centrifugation:

Ficoll-Paque ke reagent e sebelisoang haholo bakeng sa ho centrifugation ea gradient ea density ka lebaka la bokhoni ba eona ba ho theha li-gradient tse tsitsitseng. Mokhoa ona o kenyelletsa ho beha ka hloko bophahamo ba mali bo lakatsehang ho Ficoll-Paque, ho lateloa ke centrifugation ka lebelo le laoloang. Ka lebaka leo, li-RBC li theha sehlopha se ikhethang ka tlase ho plasma, e leng se nolofalletsang karohano e bonolo.

IV. Ho Ntlafatsa Diparamitha tsa Centrifugation bakeng sa Karohano e Ntlafetseng:

Ho fihlella karohano e sebetsang hantle ea li-RBC ho tsoa maling a felletseng, ntlafatso ea liparamente tsa centrifugation e bohlokoa. Lintlha tse kang lebelo, nako le mocheso li bapala karolo ea bohlokoa ho fumaneng katleho ea karohano. Ka ho lokisa li-variable tsena, bafuputsi ba ka ntlafatsa chai le bohloeki ba li-RBC tse fumanoeng maling a felletseng.

V. Ho hlaha ha Microfluidics le Theknoloji ea Lab-on-a-Chip:

Lilemong tsa morao tjena, mahlale a microfluidics le lab-on-a-chip a hlahile e le mekhoa e meng e tšepisang ho fapana le mekhoa e tloaelehileng ea centrifugation. Mekhoa ena ea sejoale-joale e kopanya lisebelisoa tse nyane tse laolang mali a manyane, li sebelisa melao-motheo e kang phallo ea hydrodynamic, matla a molumo, le mekhoa ea ho sefa ho arola li-RBC maling a felletseng. Mekhoa ena ea microscale e fana ka melemo e 'maloa, ho kenyeletsoa le litlhoko tse fokotsehileng tsa bophahamo ba sampole, phallo e ntlafalitsoeng, le taolo e ntlafalitsoeng holim'a liparamente tsa karohano.

VI. Ho Hlophisa Li-RBC ka Microfluidic ho Thehiloe ho Thepa ea Biophysical:

Li-platform tsa microfluidic li lumella karohano e nepahetseng ea li-RBC ka ho sebelisa thepa ea tsona ea biophysical. Ka ho rala li-microchannel tse nang le li-geometri tse ikhethileng le ho sebelisa mekhoa e kang karohano e bakoang ke ho se sebetse hantle, ho tsepamisa maikutlo ho sa sebetseng hantle, kapa dielectrophoresis, hoa khoneha ho hlophisa li-RBC ho latela litšobotsi tse kang boholo, sebopeho le ho tiea. Lisebelisoa tsena tsa microfluidic li fana ka ho hlaka ho hoholo ho aroleng li-RBC le ho nolofalletsa lits'ebetso tse tlase ho tlhahlobo, meriana ea tšelo ea mali le lipatlisiso.

Qetello:

Ho arola li-RBC maling a felletseng ke mohato oa bohlokoa lits'ebetsong tse ngata tsa bongaka le lipatlisiso. Mekhoa ea ho centrifugation, joalo ka ho centrifugation ea differential density gradient ho sebelisa Ficoll-Paque, e sebelisitsoe haholo bakeng sa morero ona. Leha ho le joalo, tsoelo-pele ea morao tjena ho microfluidics le theknoloji ea lab-on-a-chip e fana ka mekhoa e meng e khahlisang bakeng sa karohano ea li-RBC, e fanang ka ts'ebetso e eketsehileng, litlhoko tse fokotsehileng tsa bophahamo ba sampole, le taolo e ntlafalitsoeng ts'ebetsong ea karohano. Ha lipatlisiso lefapheng lena li ntse li tsoela pele, mekhoa ena e mecha e na le bokhoni bo boholo ba ho fetola mekhoa ea karohano ea mali le ho ntlafatsa masimo a fapaneng a lipatlisiso tsa bongaka le tsa saense.