yuav ua li cas cais cov qe ntshav liab ntawm cov centrifuge ntshav tag nrho
Cov Txuj Ci Tshiab Los Cais Cov Qe Ntshav Liab Los Ntawm Cov Ntshav Tag Nrho
Kev Taw Qhia:
Ntshav yog ib yam kua dej tseem ceeb thiab muaj ntau yam sib xyaws ua ke, suav nrog cov qe ntshav liab (RBCs), cov qe ntshav dawb, cov ntshav plasma, thiab cov ntshav platelets. Hauv qee yam kev kho mob thiab kev tshawb fawb, nws yuav tsum tau cais cov khoom ntshav tshwj xeeb, xws li RBCs, ntawm tag nrho cov ntshav. Ib qho ntawm cov txheej txheem zoo tshaj plaws rau kev ua tiav qhov kev sib cais no yog siv lub centrifuge. Hauv tsab xov xwm no, peb yuav tshawb nrhiav cov txheej txheem tshiab sib txawv uas muaj los cais RBCs ntawm tag nrho cov ntshav siv centrifugation.
I. Kev Nkag Siab Txog Cov Txheej Txheem Centrifugation:
Kev centrifugation yog ib txoj kev siv cov ntsiab cai ntawm kev tso dej thiab lub zog centrifugal los cais cov khoom me me ntawm ntau qhov sib txawv. Los ntawm kev tig cov qauv ntawm qhov ceev ceev, cov khoom me me, zoo li RBCs, tso cov av mus rau hauv qab, ua rau kev sib cais ntawm lwm cov khoom.
II. Siv Kev Sib Txawv Qhov Ceev Gradient Centrifugation:
Ib qho ntawm cov txheej txheem siv dav tshaj plaws los cais cov RBCs ntawm cov ntshav tag nrho yog qhov sib txawv ntawm qhov ceev gradient centrifugation. Txoj kev no siv qhov sib txawv ntawm qhov ceev ntawm ntau yam khoom ntawm cov ntshav. Los ntawm kev muab cov qauv tso rau saum cov khoom siv density gradient, cov khoom sib cais raws li lawv qhov ceev tshwj xeeb.
III. Siv Ficoll-Paque Density Gradient Centrifugation:
Ficoll-Paque yog ib qho tshuaj siv dav dav rau kev centrifugation density gradient vim nws muaj peev xwm tsim cov gradients ruaj khov. Cov txheej txheem no suav nrog kev ua tib zoo muab cov ntshav tag nrho uas xav tau rau ntawm Ficoll-Paque, tom qab ntawd centrifugation ntawm qhov ceev thiab lub sijhawm tswj tau. Yog li ntawd, RBCs tsim ib txoj kab sib txawv hauv qab plasma, ua rau yooj yim sib cais.
IV. Kev Txhim Kho Cov Kev Ntsuas Centrifugation rau Kev Sib Cais Zoo Dua:
Yuav kom ua tiav kev sib cais cov RBCs ntawm cov ntshav tag nrho, kev ua kom zoo tshaj plaws ntawm cov kev ntsuas centrifugation yog qhov tseem ceeb heev. Cov yam xws li qhov ceev, lub sijhawm, thiab qhov kub ua lub luag haujlwm tseem ceeb hauv kev txiav txim siab qhov ua tau zoo ntawm kev sib cais. Los ntawm kev kho cov kev hloov pauv no, cov kws tshawb fawb tuaj yeem txhim kho qhov txiaj ntsig thiab kev huv ntawm RBCs tau los ntawm cov ntshav tag nrho.
V. Kev Tshwm Sim ntawm Microfluidics thiab Lab-on-a-Chip Technologies:
Nyob rau xyoo tas los no, cov thev naus laus zis microfluidics thiab cov thev naus laus zis hauv chav kuaj tau tshwm sim los ua lwm txoj hauv kev zoo rau cov txheej txheem centrifugation ib txwm muaj. Cov txheej txheem txiav tshiab no suav nrog cov khoom siv me me uas tswj cov ntshav me me, siv cov ntsiab cai xws li hydrodynamic flow, acoustic forces, thiab cov txheej txheem lim dej los cais RBCs ntawm cov ntshav tag nrho. Cov txheej txheem microscale no muaj ntau yam zoo, suav nrog kev txo qis qhov yuav tsum tau muaj cov qauv, kev txhim kho throughput, thiab kev tswj hwm zoo dua ntawm cov kev sib cais.
VI. Kev faib cov qe ntshav liab me me raws li cov khoom siv biophysical:
Cov platform microfluidic tso cai rau kev sib cais ntawm RBCs kom meej los ntawm kev siv lawv cov khoom biophysical. Los ntawm kev tsim cov microchannels nrog cov geometries tshwj xeeb thiab siv cov txheej txheem xws li kev sib cais deformability-induced, inertial focusing, lossis dielectrophoresis, nws muaj peev xwm los cais RBCs raws li cov yam ntxwv xws li qhov loj me, cov duab, thiab kev tawv. Cov khoom siv microfluidic no muab kev tshwj xeeb siab hauv kev cais RBCs thiab ua rau cov ntawv thov downstream hauv kev kuaj mob, kev kho mob transfusion, thiab kev tshawb fawb.
Xaus lus:
Kev cais cov ntshav liab (RBCs) ntawm cov ntshav tag nrho yog ib kauj ruam tseem ceeb hauv ntau yam kev siv kho mob thiab kev tshawb fawb. Cov txheej txheem centrifugation, xws li kev sib txawv ntawm qhov ceev gradient centrifugation siv Ficoll-Paque, tau siv dav rau lub hom phiaj no. Txawm li cas los xij, kev nce qib tsis ntev los no hauv microfluidics thiab lab-on-a-chip technologies muab cov kev xaiv zoo rau kev sib cais RBC, muab kev ua haujlwm zoo dua, txo qhov yuav tsum tau ntim cov qauv, thiab txhim kho kev tswj hwm ntawm cov txheej txheem sib cais. Thaum kev tshawb fawb hauv daim teb no txuas ntxiv mus, cov txheej txheem tshiab no muaj peev xwm zoo rau kev hloov pauv txoj hauv kev sib cais ntshav thiab txhim kho ntau yam kev tshawb fawb kho mob thiab kev tshawb fawb.
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