indlela yokuhlukanisa amaseli abomvu egazi ku-centrifuge yegazi lonke

Amasu Antsha Okuhlukanisa Amaseli Egazi Abomvu Egazini Eliphelele

Isingeniso:

Igazi liwuketshezi oluyinkimbinkimbi nolubalulekile oluqukethe izingxenye ezahlukene, okuhlanganisa amangqamuzana abomvu egazi (ama-RBC), amangqamuzana amhlophe egazi, i-plasma, nama-platelet. Kwezinye izinhlelo zokusebenza zezokwelapha nezocwaningo, kuba nesidingo sokuhlukanisa izingxenye ezithile zegazi, njenge-RBC, egazini lonke. Enye yezindlela eziphumelela kakhulu zokufeza lokhu kuhlukaniswa ukusebenzisa i-centrifuge. Kulesi sihloko, sizohlola amasu ahlukene amasha atholakalayo okuhlukanisa ama-RBC egazini lonke sisebenzisa i-centrifugation.

I. Ukuqonda Inqubo Yokucisha Ugesi:

I-Centrifugation iyindlela yokusebenzisa izimiso zokuthuthwa kanye namandla e-centrifugal ukuhlukanisa izinhlayiya ezinobukhulu obuhlukene. Ngokuphotha isampula ngesivinini esikhulu, izinhlayiya eziqinile, njenge-RBC, zithuthwa ziye phansi, okuvumela ukuhlukana kwezinye izingxenye.

II. Ukusebenzisa i-Differential Density Gradient Centrifugation:

Enye yezindlela ezisetshenziswa kakhulu ukuhlukanisa ama-RBC egazini lonke i-differential density gradient centrifugation. Le ndlela isebenzisa umehluko wobuningi phakathi kwezingxenye ezahlukene zegazi. Ngokubeka isampula phezu kwe-density gradient medium, izakhi ziyahlukana ngokusekelwe ekubuneni kwazo okuthile.

III. Ukusebenzisa i-Ficoll-Paque Density Gradient Centrifugation:

I-Ficoll-Paque iyi-reagent esetshenziswa kabanzi ekugxilweni kwe-density gradient ngenxa yekhono layo lokwakha ama-gradient azinzile. Le ndlela ihilela ukubeka ngokucophelela ivolumu efiselekayo yegazi lonke ku-Ficoll-Paque, kulandelwe yi-centrifugation ngesivinini esilawulwayo kanye nobude besikhathi. Ngenxa yalokho, ama-RBC akha ibhande elihlukile ngaphansi kwe-plasma, okwenza kube lula ukuhlukana.

IV. Ukuthuthukisa Amapharamitha E-Centrifugation Ukuze Kuhlukaniswe Okuthuthukisiwe:

Ukuze kufezwe ukuhlukaniswa okuphumelelayo kwama-RBC egazini eliphelele, ukwenziwa ngcono kwamapharamitha okusebenza kwe-centrifugation kubalulekile. Izici ezifana nesivinini, ubude, kanye nokushisa kudlala indima ebalulekile ekunqumeni ukusebenza kahle kokuhlukaniswa. Ngokulungisa lezi ziguquguquko, abacwaningi bangathuthukisa isivuno kanye nobumsulwa bama-RBC atholakala egazini eliphelele.

V. Ukufika Kwe-Microfluidics kanye Nezobuchwepheshe Be-Lab-on-a-Chip:

Eminyakeni yamuva nje, ubuchwepheshe be-microfluidics kanye ne-lab-on-a-chip buvele njengezindlela ezithembisayo zokusebenzisa izindlela ezivamile zokuhlukanisa igazi. Lezi zindlela ezisezingeni eliphezulu zihlanganisa amadivayisi amancane alawula inani elincane legazi, esebenzisa izimiso ezifana nokugeleza kwe-hydrodynamic, amandla e-acoustic, kanye nezindlela zokuhlunga ukuze kuhlukaniswe ama-RBC egazini eliphelele. Lezi zindlela ze-microscale zinikeza izinzuzo eziningana, kufaka phakathi izidingo zomthamo wesampula oncishisiwe, ukudluliswa okuthuthukisiwe, kanye nokulawula okuthuthukisiwe kwamapharamitha okuhlukaniswa.

VI. Ukuhlungwa kwama-RBC nge-Microfluidic Ngokusekelwe Ezimpahleni Ze-Biophysical:

Amapulatifomu e-Microfluidic avumela ukuhlukaniswa okunembile kwama-RBC ngokusebenzisa izakhiwo zawo ze-biophysical. Ngokuklama iziteshi ezincane ezine-geometries ethile nokusebenzisa amasu afana nokuhlukaniswa okubangelwa ukukhubazeka, ukugxila kwe-inertial, noma i-dielectrophoresis, kungenzeka ukuhlunga ama-RBC ngokusekelwe ezicini ezifana nosayizi, ukuma, nokuqina. Lawa madivayisi e-microfluidic anikeza ukucaciswa okuphezulu ekuhlukaniseni ama-RBC futhi avumela ukusetshenziswa okungezansi ekuxilongeni, ekwelashweni kokumpompela igazi, kanye nocwaningo.

Isiphetho:

Ukuhlukanisa ama-RBC egazini eliphelele kuyisinyathelo esibalulekile ezicelweni eziningi zezokwelapha nezocwaningo. Amasu okusebenzisa i-centrifugation, njenge-differential density gradient centrifugation esebenzisa i-Ficoll-Paque, asetshenziswe kabanzi kule njongo. Kodwa-ke, intuthuko yakamuva kubuchwepheshe be-microfluidics kanye ne-lab-on-a-chip inikeza ezinye izindlela ezithakazelisayo zokuhlukanisa ama-RBC, okuhlinzeka ngokusebenza kahle okukhulu, izidingo zesampula ezincishisiwe, kanye nokulawula okuthuthukisiwe kwenqubo yokuhlukanisa. Njengoba ucwaningo kulo mkhakha luqhubeka nokuthuthuka, lawa masu amasha anamandla amakhulu okuguqula izindlela zokuhlukanisa igazi nokuthuthukisa amasimu ahlukahlukene ocwaningo lwezokwelapha nolwesayensi.