Kodi chisakanizo chofanana chingalekanitsidwe ndi centrifugation

Kutsegula Mitsempha: Njira Yothetsera Kusakaniza Kofanana

Chiyambi:

Zosakaniza ndi mfundo yaikulu mu chemistry, zomwe zimapanga kuphatikiza kwa zinthu ziwiri kapena zingapo. Zitha kugawidwa m'magulu ngati zofanana kapena zosiyana kutengera mawonekedwe awo akuthupi. Mu zosakaniza zofanana, zigawozo zimagawidwa mofanana ndipo sizingasiyanitsidwe ndi maso. Mosiyana ndi zimenezi, zosakaniza zosiyana zimakhala ndi zinthu zosiyana kwambiri. Kulekanitsa zosakaniza ndi njira yofunika kwambiri pa ntchito zosiyanasiyana zasayansi ndi mafakitale. Njira imodzi yodziwika bwino yomwe imagwiritsidwa ntchito pachifukwa ichi ndi centrifugation. Nkhaniyi ikufotokoza momwe centrifugation imagwirira ntchito polekanitsa zosakaniza zofanana pamene ikuwunikira mfundo zake.

Kodi Centrifugation ndi chiyani?

Kugawanitsa zinthu ndi njira yolekanitsira zinthu pogwiritsa ntchito mphamvu ya centrifugal, yomwe imapangitsa kuti zinthu zigawike mofulumira kapena kugawanitsa zinthu mkati mwa chisakanizocho. Pamene chisakanizocho chikuzungulira mofulumira, zinthu zokhuthala kwambiri zimayendetsedwa pansi pomwe zinthu zosakhuthala kwambiri zimasonkhana pamwamba. Pogwiritsa ntchito kusiyana kumeneku, kugawa zinthu pogwiritsa ntchito centrifugal kumatha kugawanitsa zinthu zovuta mosavuta.

Mfundo ya Centrifugation:

Mphamvu ya centrifugal, mphamvu yonyenga, imapangidwa chifukwa cha kuzungulira kwa chitsanzo mkati mwa centrifuge. Mphamvu iyi imagwira ntchito kunja kuchokera pakati pa kuzungulira ndipo imagwirizana ndi kulemera kwa tinthu tating'onoting'ono tomwe tili mu chisakanizocho. Malinga ndi lamulo loyamba la Newton, chinthu chomwe chili pamalopo chimakhala pamalopo, ndipo chinthu chomwe chikuyenda chimakhala pamalopo, pokhapokha ngati chikugwiritsidwa ntchito ndi mphamvu yakunja. Mu centrifugation, mphamvu yakunja iyi ndi mphamvu ya centrifugal, yomwe imagwira ntchito kunja, zomwe zimayambitsa kusungunuka kapena kulekanitsidwa kwa zigawo kutengera kuchulukana kwawo.

Mutu Waufupi 1: Kusakaniza kwa Zosakaniza Zofanana

Ambiri amakhulupirira kuti kugawa kwa centrifugation sikuthandiza pakugawanitsa zosakaniza zofanana chifukwa cha kuchulukana kwawo kofanana. Komabe, pazifukwa zina, kugawa kumeneku kumakhala kotheka. Zosakaniza zofanana nthawi zambiri zimakhala ndi tinthu tating'onoting'ono kapena mamolekyu okhala ndi kukula kapena mawonekedwe osiyanasiyana, zomwe zimapangitsa kuti pakhale kusiyana kwa kuchuluka kwa dothi. Mwa kusankha magawo oyenera a centrifugation monga liwiro ndi nthawi, ndizotheka kuwona kusiyana kwakukulu.

Mutu Wachiwiri: Kulekanitsa Ma Colloids Pogwiritsa Ntchito Centrifugation

Ma Colloidal suspensions ndi zitsanzo za zosakaniza zofanana zomwe zimapangitsa kuti pakhale vuto lolekanitsa. Ma Colloid nthawi zambiri amakhala ndi tinthu tating'onoting'ono tomwe timapachikidwa mkati mwa madzi, zomwe zimapangitsa kuti pakhale mawonekedwe osiyanasiyana. Ngakhale njira zachikhalidwe zosefera sizingalekanitse bwino zosakanizazi, centrifugation imagwira ntchito bwino. Mwa kuzunguliza colloid pa liwiro lalikulu, tinthu tating'onoting'ono titha kukakamizika kutayika, zomwe pamapeto pake zimapangitsa kuti colloid ipatukane bwino.

Mutu Waukulu 3: Kuchepetsa Kuchuluka kwa Ma Gradient kwa Zosakaniza Zofanana

Kuchuluka kwa ma centrifugation ndi njira yapadera yomwe imagwiritsidwa ntchito kulekanitsa zosakaniza zomwe zili ndi ma densities ofanana. Mu njira iyi, njira yocheperako monga sucrose kapena cesium chloride solution imakonzedwa, yomwe imapanga ma density gradient motsatira kutalika kwa chubu cha centrifuge kapena rotor. Kenako chisakanizocho chimayikidwa pa gradient, ndipo centrifugation imachitika. Pamene chisakanizocho chikuyenda kudzera mu ma density gradient, zigawo zomwe zili ndi ma densities osiyanasiyana zimakhazikika pamalo awo, zomwe zimathandiza kupatukana.

Mutu Waufupi 4: Kutsegula Ma Centrifugation mu Makampani Opanga Mankhwala

Makampani opanga mankhwala amagwiritsa ntchito kwambiri njira yolekanitsira zinthu pogwiritsa ntchito centrifugation ngati njira yolekanitsira zinthu. Zosakaniza zofanana zomwe zimapezeka nthawi zambiri mu kafukufuku wamankhwala, monga njira zopezera mapuloteni, zimafuna kulekanitsa kuti ziyeretsedwe. Kulekanitsa zinthu pogwiritsa ntchito centrifugation kumachita gawo lofunika kwambiri pakulekanitsa mapuloteni ndi ma biomolecule kutengera kulemera kwawo kapena kuchuluka kwawo. Njirayi imatsimikizira kupanga mankhwala abwino kwambiri pochotsa zodetsa ndikuchotsa zinthu zomwe zikufuna.

Mutu Waufupi 5: Zofooka za Centrifugation

Ngakhale kuti centrifugation ndi njira yosiyana yolekanitsira zinthu, ili ndi zofooka zake pochita zinthu zina zosakanikirana mofanana. Mwachitsanzo, m'zosakaniza zomwe kukula kwa tinthu tating'onoting'ono kuli kofanana kwambiri kapena kuchulukana kuli kofanana kwambiri, centrifugation singapereke zotsatira zabwino. Muzochitika zotere, njira zina zolekanitsira zinthu, monga chromatography kapena kusefa, ziyenera kuganiziridwa kuti zikwaniritse zomwe mukufuna.

Mapeto:

Kugawa zinthu pogwiritsa ntchito centrifugation ndi njira yamphamvu yolekanitsira zinthu zosakaniza, kuphatikizapo machitidwe ofanana. Pogwiritsa ntchito kusiyana kwa kuchuluka kwa zinthu kapena kukula kwa tinthu, kugawa zinthu pogwiritsa ntchito centrifugation kumatha kusiyanitsa bwino zinthu zovuta zomwe zingakhale zovuta kuzigawa pogwiritsa ntchito njira zachikhalidwe. Ngakhale kuti nthawi zina sizingakhale chisankho chabwino kwambiri pa zinthu zonse zofanana, kumvetsetsa mfundo ndi zofooka za kugawa zinthu pogwiritsa ntchito centrifugation kumathandiza asayansi ndi ofufuza kupanga zisankho zolondola za njira zoyenera zogawa zinthu pogwiritsa ntchito njira zawo.