musanganiswa wakafanana unogona kuparadzaniswa ne centrifugation

Kuparadzanisa Misanganiswa Yakafanana: Mhinduro Yekuparadzanisa Misanganiswa Yakafanana

Nhanganyaya:

Misanganiswa ipfungwa huru mukemistri, inoumba musanganiswa wezvinhu zviviri kana kupfuura. Zvinogona kurongwa sezvine homogeneous kana heterogeneous zvichienderana nechitarisiko chazvo chemuviri. Mumusanganiswa wakafanana, zvikamu zvacho zvakapararira zvakaenzana uye hazvigone kusiyaniswa nemaziso. Kusiyana neizvi, musanganiswa wakafanana une zvinhu zvakasiyana zvinooneka. Kuparadzanisa misanganiswa inzira yakakosha mumashandisirwo akasiyana esainzi nemaindasitiri. Imwe nzira yakajairika inoshandiswa pachinangwa ichi ndeye centrifugation. Chinyorwa chino chinoongorora kushanda kwe centrifugation mukuparadzanisa misanganiswa yakaenzana uku uchijekesa misimboti yayo.

Chii chinonzi Centrifugation?

Kutenderedza zvinhu (centrifugation) inzira yekuparadzanisa inoshandisa kutenderera nekukurumidza kuparadzanisa musanganiswa zvichienderana nekusiyana kwehuwandu kana saizi yezvikamu. Inoenderana nekushandiswa kwesimba repakati, iro rinokurumidza kudonha kwemvura kana kuparadzanisa zvikamu zviri mumusanganiswa. Sezvo musanganiswa uchitenderera nekukurumidza, zvikamu zvakawandisa zvinofambiswa zvichienda pasi nepo zvinhu zvishoma zvakakora zvichiungana pamusoro. Nekushandisa misiyano iyi, kutenderedza zvinhu kunogona kuparadzanisa musanganiswa wakaomarara zvinobudirira.

Nheyo yeCentrifugation:

Simba reCentrifugal, simba rekunyepedzera, rinogadzirwa nekuda kwekutenderera kwemuenzaniso mukati me centrifuge. Simba iri rinobuda kunze kubva pakati pekutenderera uye rinoenderana nehuremu hwezvikamu zviri mumusanganiswa. Sekureva kwemutemo wekutanga waNewton, chinhu chiri pakamira chinowanzo gara chakazorora, uye chinhu chiri kufamba chinowanzo ramba chichifamba, kunze kwekunge chashandiswa nesimba rekunze. Mu centrifugation, simba iri rekunze isimba recentrifugal, rinoshanda richibuda kunze, izvo zvinokonzera kudonha kana kupatsanurwa kwezvikamu zvichienderana nekuwanda kwazvo.

Musoro wenyaya 1: Kubatanidza Misanganiswa Yakafanana

Vazhinji vanotenda kuti centrifugation haishande mukuparadzanisa misanganiswa yakafanana nekuda kwekuwanda kwayo kwakafanana. Zvisinei, pasi pemamwe mamiriro ezvinhu, kupatsanurwa uku kunogoneka. Misanganiswa yakafanana inowanzo kuve nezvidimbu kana mamorekuru ane saizi dzakasiyana kana maumbirwo, zvichikonzera kusiyana kwehuwandu hwesedimentation. Nekusarudza maparamita akakodzera ecentrifugation akadai sekumhanya nenguva, zvinokwanisika kuona kupatsanurwa kwakakosha.

Musoro weChinyorwa 2: Kuparadzanisa Colloids Uchishandisa Centrifugation

Kusanganiswa kweColloidal mienzaniso yemisanganiswa yakafanana iyo inonetsa kupatsanurana. Colloid inowanzo kuve nezvidimbu zvidiki-diki zvakasungirirwa mukati memvura, zvichikonzera chitarisiko chakasiyana. Kunyange zvazvo nzira dzechinyakare dzekusefa dzingasaparadzanisa misanganiswa iyi zvakakwana, centrifugation inoratidza kuti inoshanda. Nekutenderedza colloid nekumhanya kwakanyanya, zvidimbu zvakawandisa zvinogona kumanikidzwa kudonha, zvichizopedzisira zvaita kuti colloid iparadzane zvakajeka.

Musoro weChikamu 3: Kudzikamisa Density Gradient kweMisanganiswa Yakafanana

Kuyera density gradient centrifugation inzira yakasarudzika inoshandiswa kupatsanura musanganiswa une densities dzakafanana. Munzira iyi, density gradient medium yakaita se sucrose kana cesium chloride solution inogadzirwa, iyo inogadzira density gradient pamwe chete nehurefu hwe centrifuge tube kana rotor. Musanganiswa wacho unozoiswa pamusoro pe gradient, uye centrifugation inoitiswa. Sezvo musanganiswa uchifamba nepakati pe density gradient, zvikamu zvine densities dzakasiyana zvinogara panzvimbo dzazvo, zvichiita kuti kupatsanurwa kuve nyore.

Musoro wenyaya 4: Kugadzira Centrifugation muIndasitiri yeMishonga

Indasitiri yemishonga inoshandisa zvakanyanya centrifugation senzira yekuparadzanisa. Misanganiswa yakafanana inowanzo kuwanikwa mukutsvagisa mishonga, senge mhinduro dzemapuroteni, inoda kupatsanurwa kuti icheneswe. Centrifugation inoita basa rakakosha mukuparadzanisa mapuroteni nema biomolecule zvichienderana nehuremu hwawo kana huwandu hwawo. Iyi nzira inovimbisa kugadzirwa kwezvigadzirwa zvemishonga zvemhando yepamusoro nekubvisa tsvina uye kuparadzanisa zvinhu zvinovavarirwa.

Musoro weChinyorwa 5: Kuganhurirwa kweCentrifugation

Kunyange hazvo centrifugation iri nzira inoshandiswa zvakasiyana-siyana yekuparadzanisa, ine zvipingamupinyi zvayo pakubata nemimwe misanganiswa yakafanana. Semuenzaniso, mumisanganiswa umo saizi dzezvimedu dzakafanana zvakanyanya kana kuti huwandu hwacho hwakafanana, centrifugation inogona kusapa mhedzisiro inogutsa. Muzviitiko zvakadaro, dzimwe nzira dzekuparadzanisa, dzakadai se chromatography kana filtration, dzinofanira kufungwa kuti dzisvike pamhedzisiro inodiwa.

Mhedziso:

Kuparadzanisa zvinhu zvakasiyana-siyana (centrifugation) inzira ine simba yekuparadzanisa zvinhu zvakasiyana-siyana, kusanganisira masisitimu akafanana. Nekushandisa musiyano muhuwandu kana hukuru hwezvinhu zvakasiyana-siyana, kuparadzanisa zvinhu zvakasiyana-siyana kunogona kuparadzanisa zvinhu zvakaoma-siyana izvo zvaizove zvakaoma kuparadzanisa uchishandisa nzira dzechinyakare. Kunyangwe zvingasava sarudzo yakanaka kune musanganiswa wega wega wakafanana, kunzwisisa misimboti nemiganhu yekubatanidza zvinhu kunoita kuti masayendisiti nevaongorori vasarudze zvine ruzivo nezvenzira dzakakodzera dzekuparadzanisa zvinhu.