puas tuaj yeem sib cais cov khoom sib xyaw ua ke los ntawm centrifugation
Centrifugation: Ib qho kev daws teeb meem rau kev sib cais cov khoom sib xyaw ua ke
Kev Taw Qhia:
Cov khoom sib xyaw yog lub tswv yim tseem ceeb hauv chemistry, uas yog kev sib xyaw ua ke ntawm ob lossis ntau yam khoom. Lawv tuaj yeem raug faib ua homogeneous lossis heterogeneous raws li lawv qhov tsos. Hauv cov khoom sib xyaw homogeneous, cov khoom sib xyaw tau faib sib npaug thiab tsis tuaj yeem pom qhov sib txawv. Ntawm qhov tod tes, cov khoom sib xyaw heterogeneous muaj cov khoom sib txawv pom tseeb. Kev sib cais cov khoom sib xyaw yog ib qho txheej txheem tseem ceeb hauv ntau yam kev tshawb fawb thiab kev siv hauv kev lag luam. Ib txoj hauv kev siv rau lub hom phiaj no yog centrifugation. Tsab xov xwm no tshawb nrhiav qhov ua tau zoo ntawm centrifugation hauv kev sib cais cov khoom sib xyaw homogeneous thaum tso lub teeb rau nws cov hauv paus ntsiab lus.
Centrifugation yog dab tsi?
Kev sib xyaw ua ke (centrifugation) yog ib txoj kev sib cais uas siv kev sib hloov ceev ceev los cais cov khoom sib xyaw raws li qhov sib txawv ntawm qhov ceev lossis qhov loj ntawm cov khoom me me. Nws vam khom kev siv lub zog centrifugal, uas ua rau cov khoom sib xyaw sai dua lossis cais cov khoom hauv cov khoom sib xyaw. Thaum cov khoom sib xyaw tig sai sai, cov khoom sib xyaw tuab dua raug tsav mus rau hauv qab thaum cov khoom sib xyaw tsawg dua sib sau ua ke rau saum toj. Los ntawm kev siv cov kev sib txawv no, kev sib xyaw ua ke tuaj yeem cais cov khoom sib xyaw nyuaj tau zoo.
Lub hauv paus ntsiab lus ntawm centrifugation:
Lub zog centrifugal, lub zog pseudo, yog tsim los ntawm kev tig ntawm cov qauv hauv lub centrifuge. Lub zog no ua haujlwm sab nraud ntawm qhov chaw ntawm kev tig thiab yog proportional rau qhov hnyav ntawm cov khoom me me hauv qhov sib tov. Raws li Newton txoj cai thawj zaug, ib yam khoom uas so feem ntau nyob twj ywm, thiab ib yam khoom uas txav mus los feem ntau nyob twj ywm, tshwj tsis yog tias muaj lub zog sab nraud ua rau. Hauv centrifugation, lub zog sab nraud no yog lub zog centrifugal, ua haujlwm radially sab nraud, uas ua rau muaj kev sib cais lossis sib cais ntawm cov khoom raws li lawv qhov ceev.
Nqe Lus Qhia Me 1: Kev Centrifugation ntawm Cov Khoom Sib Xyaws Sib Xws
Muaj coob tus ntseeg tias kev siv centrifugation tsis muaj txiaj ntsig zoo hauv kev sib cais cov khoom sib xyaw ua ke vim lawv cov ntom zoo sib xws. Txawm li cas los xij, nyob rau hauv qee qhov xwm txheej, qhov kev sib cais no ua tau. Cov khoom sib xyaw ua ke feem ntau muaj cov khoom me me lossis cov molecule nrog ntau qhov ntau thiab tsawg lossis cov duab sib txawv, ua rau muaj kev hloov pauv ntawm cov nqi sedimentation. Los ntawm kev xaiv cov kev ntsuas centrifugation tsim nyog xws li qhov ceev thiab lub sijhawm, nws muaj peev xwm pom kev sib cais tseem ceeb.
Nqe Lus Qhia Ntxiv 2: Kev Cais Cov Colloids Siv Centrifugation
Cov tshuaj Colloidal suspensions yog cov piv txwv ntawm cov khoom sib xyaw ua ke uas ua rau muaj kev sib tw rau kev sib cais. Cov Colloids feem ntau muaj cov khoom me me uas dai rau hauv cov kua nruab nrab, ua rau muaj qhov sib txawv. Txawm hais tias cov txheej txheem lim dej ib txwm yuav tsis cais cov khoom sib xyaw no kom txaus, centrifugation ua pov thawj tias muaj txiaj ntsig zoo. Los ntawm kev tig cov colloid ntawm qhov ceev ceev, cov khoom me me tuaj yeem raug yuam kom ua rau cov av noo, thaum kawg ua rau muaj kev sib cais meej ntawm cov colloid.
Lub Ncauj Lus Me 3: Kev Siv Centrifugation Gradient rau Cov Khoom Sib Xyaws Sib Xws
Kev siv centrifugation los cais cov khoom sib xyaw uas muaj qhov ceev sib xws yog ib txoj kev tshwj xeeb uas siv los cais cov khoom sib xyaw uas muaj qhov ceev sib xws. Hauv txoj kev no, ib qho khoom siv los cais cov khoom sib xyaw xws li cov kua sucrose lossis cesium chloride raug npaj, uas tsim ib qho kev faib tawm ntawm qhov ceev raws li qhov ntev ntawm lub raj centrifuge lossis rotor. Tom qab ntawd cov khoom sib xyaw raug muab tso rau ntawm qhov sib txawv, thiab kev centrifugation raug ua. Thaum cov khoom sib xyaw txav mus los ntawm qhov sib txawv ntawm qhov ceev, cov khoom sib xyaw uas muaj qhov ceev sib txawv nyob rau ntawm lawv qhov chaw, ua rau kev sib cais yooj yim.
Nqe Lus Qhia Me 4: Kev Siv Centrifugation Hauv Kev Lag Luam Tshuaj
Kev lag luam tshuaj siv ntau yam kev centrifugation ua ib txoj kev sib cais. Cov khoom sib xyaw ua ke uas feem ntau pom hauv kev tshawb fawb tshuaj, xws li cov kua protein, xav tau kev sib cais rau lub hom phiaj ntawm kev ntxuav. Kev centrifugation ua lub luag haujlwm tseem ceeb hauv kev sib cais cov protein thiab biomolecules raws li lawv qhov hnyav molecular lossis qhov ceev. Cov txheej txheem no ua kom ntseeg tau tias muaj cov khoom lag luam tshuaj zoo los ntawm kev tshem tawm cov khoom tsis huv thiab cais cov tshuaj sib xyaw.
Nqe Lus Qhia Ntxiv 5: Cov Kev Txwv ntawm Centrifugation
Txawm hais tias centrifugation yog ib txoj kev sib cais uas siv tau ntau yam, nws muaj nws cov kev txwv thaum cuam tshuam nrog qee cov khoom sib xyaw ua ke. Piv txwv li, hauv cov khoom sib xyaw uas qhov loj me ntawm cov khoom me me zoo sib xws lossis qhov ceev yuav luag zoo ib yam, centrifugation yuav tsis xa cov txiaj ntsig zoo. Hauv cov xwm txheej zoo li no, lwm txoj hauv kev sib cais, xws li chromatography lossis filtration, yuav tsum tau txiav txim siab kom ua tiav qhov tshwm sim xav tau.
Xaus lus:
Kev siv centrifugation yog ib txoj kev siv zog heev rau kev sib cais cov khoom sib xyaw, suav nrog cov txheej txheem sib xyaw ua ke. Los ntawm kev siv qhov sib txawv ntawm qhov ceev lossis qhov loj ntawm cov khoom me me, kev siv centrifugation tuaj yeem sib cais cov khoom sib xyaw ua ke uas yuav nyuaj rau kev sib cais siv cov txheej txheem ib txwm muaj. Txawm hais tias nws yuav tsis yog qhov kev xaiv zoo tshaj plaws rau txhua qhov sib xyaw ua ke, kev nkag siab txog cov ntsiab cai thiab kev txwv ntawm kev siv centrifugation tso cai rau cov kws tshawb fawb thiab cov kws tshawb fawb txiav txim siab txog cov txheej txheem sib cais zoo tshaj plaws rau lawv cov kev siv tshwj xeeb.
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