Kev tsim khoom tshaj lij ntawm cov centrifuge separator thiab cov chaw tsim khoom siv tshuaj - Zonelink

vim li cas cov ntshav tsis sib cais hauv lub centrifuge

2023-07-30

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Vim li cas cov ntshav tsis sib cais hauv lub tshuab centrifuge?

Kev Taw Qhia:

Ntshav yog ib feem tseem ceeb ntawm tib neeg lub cev, ua haujlwm ua txoj sia uas thauj cov pa oxygen, cov as-ham, thiab cov tshuaj hormones thoob plaws peb lub cev. Nws muaj ntau yam khoom sib txawv, suav nrog cov qe ntshav liab, cov qe ntshav dawb, cov platelets, thiab cov plasma. Thaum peb kuaj mob lossis xav tau kev hloov ntshav, nws yog ib qho tsim nyog los cais cov khoom no rau kev kuaj mob lossis kev kho mob. Centrifugation yog ib txoj kev siv ntau los ua kom tiav qhov kev sib cais no tau zoo. Txawm li cas los xij, koj puas tau xav tsis thoob tias vim li cas cov ntshav tsis sib cais hauv lub centrifuge? Hauv tsab xov xwm no, peb yuav tshawb nrhiav cov yam ntxwv uas ua rau muaj qhov xwm txheej zoo kawg no thiab cov ntsiab cai tshawb fawb uas yog lub hauv paus ntawm nws.

Cov Cheebtsam Ntshav thiab Lawv Cov Khoom

Ua ntej yuav kawm txog kev tshawb fawb txog kev sib cais ntshav, nws yog ib qho tseem ceeb kom nkag siab txog cov khoom sib xyaw thiab cov yam ntxwv ntawm nws cov khoom sib txawv.

1.1 – Cov Qog Ntshav Liab (Erythrocytes):

Cov qe ntshav liab yog cov qe ntshav uas muaj ntau tshaj plaws hauv peb cov ntshav. Lawv nqa cov pa oxygen los ntawm lub ntsws mus rau cov nqaij ntawm lub cev thiab thauj cov pa roj carbon dioxide rov qab mus rau lub ntsws kom tshem tawm. Cov qe ntshav no muaj hemoglobin, ib qho protein uas khi cov pa oxygen thiab ua rau lawv muaj xim liab.

1.2 – Cov Qog Ntshav Dawb (Leukocytes):

Cov qe ntshav dawb ua lub luag haujlwm tseem ceeb hauv peb lub cev tiv thaiv kab mob, tiv thaiv peb lub cev tiv thaiv kab mob thiab kab mob. Muaj ntau hom qe ntshav dawb sib txawv, txhua tus tshwj xeeb hauv cov txheej txheem tiv thaiv tshwj xeeb. Tsis zoo li cov qe ntshav liab, lawv muaj cov nuclei thiab tsis muaj hemoglobin.

1.3 – Cov Platelets (Thrombocytes):

Cov platelets yog cov uas ua rau cov ntshav khov thiab ua lub luag haujlwm tseem ceeb hauv kev kho cov qhov txhab. Lawv yog cov cell me me uas ncig hauv cov ntshav, tsis ua haujlwm kom txog thaum cov hlab ntsha puas tsuaj. Thaum cov platelets ua haujlwm lawm, lawv sib sau ua ke los ua cov ntshav khov, tiv thaiv kev los ntshav ntau dhau.

1.4 – Cov ntshav:

Cov ntshav (plasma) yog cov kua dej ntawm cov ntshav, uas yog li ntawm 55% ntawm nws tag nrho cov ntim. Nws feem ntau yog ua los ntawm dej tab sis kuj muaj cov electrolytes, cov protein plasma (xws li albumin thiab globulins), cov tshuaj hormones, cov enzymes, thiab cov khoom pov tseg. Nws ua haujlwm ua ib qho chaw nruab nrab rau kev thauj cov as-ham, cov tshuaj hormones, thiab cov khoom pov tseg.

Lub Ntsiab Cai ntawm Centrifugation

Centrifugation yog ib txoj kev siv lub zog centrifugal los cais cov khoom sib txawv hauv ib qho qauv.

2.1 – Tsim Lub Tshuab Centrifuge:

Cov centrifuges muaj ib lub rotor, uas tuav cov raj uas muaj cov qauv, thiab ib lub cev muaj zog uas tig lub rotor ntawm qhov ceev ceev. Lub rotor tig tsim lub zog centrifugal, thawb cov khoom hnyav dua ntawm cov qauv mus rau phab ntsa sab nraud ntawm cov raj.

2.2 - Lub zog centrifugal:

Lub zog centrifugal yog lub zog pom tseeb uas cov khoom txav mus los hauv lub voj voog. Nws raug taw qhia tawm ntawm qhov chaw nruab nrab ntawm kev tig thiab yog proportional rau qhov hnyav ntawm cov khoom thiab lub square ntawm nws qhov ceev. Hauv lub centrifuge, lub zog no tsav cov khoom ntshav sib cais.

Lub Luag Haujlwm ntawm Qhov Ceev hauv Kev Sib Cais Ntshav

3.1 – Qhov Ceev Sib Piv ntawm Cov Cheebtsam Ntshav:

Qhov zoo kawg nkaus ntawm kev sib cais cov ntshav hauv lub centrifuge yog tias cov khoom tsis sib cais ua ntau txheej sib txawv. Hloov chaw, lawv tsim cov qauv sib txawv raws li lawv qhov ceev. Cov qe ntshav liab, yog qhov hnyav tshaj plaws, cov av qis mus rau hauv qab, ua raws li cov qe ntshav dawb thiab cov platelets. Plasma tseem nyob rau sab saum toj vim nws yog cov khoom sib xyaw tsawg tshaj plaws.

3.2 - Lub Tsho Buffy:

Cov txheej nruab nrab, nyob nruab nrab ntawm cov qe ntshav liab hauv qab thiab cov plasma nyob rau sab saum toj, hu ua cov txheej buffy. Nws muaj cov qe ntshav dawb thiab cov platelets sib xyaw. Txawm hais tias lawv qhov ceev tsawg dua piv rau cov qe ntshav liab, lawv tsis faib thoob plaws hauv cov plasma vim muaj kev sib koom ua ke ntawm ntau lub zog.

Cov Yam Tseem Ceeb ntawm Centrifugation Uas Cuam Tshuam Rau Kev Sib Cais Ntshav

4.1 – Qhov Ceev thiab Lub Sijhawm ntawm Centrifuge:

Qhov ceev uas lub rotor tig thiab lub sijhawm ntawm centrifugation ua lub luag haujlwm tseem ceeb hauv kev sib cais cov khoom ntshav. Kev ceev dua thiab lub sijhawm ntev dua ua rau muaj kev sib cais zoo dua, vim tias lub zog centrifugal raug siv rau lub sijhawm ntev dua.

4.2 – Tsim Lub Raj:

Kev tsim cov raj kuj cuam tshuam rau kev sib cais ntawm cov ntshav. Cov raj tshwj xeeb uas muaj cov khoom siv gradient lossis cov gel inserts tuaj yeem pab ua kom tau qhov sib cais meej dua, txo qhov tsim ntawm cov txheej buffy thiab ua kom pom tseeb ntawm cov txheej sib txawv.

Viscoelasticity thiab Lwm Yam Yam Ua Si

5.1 - Viscoelasticity ntawm cov ntshav:

Cov ntshav muaj cov yam ntxwv viscoelastic, uas txhais tau tias nws muaj cov yam ntxwv ntawm cov kua thiab cov khoom khov. Cov yam ntxwv no cuam tshuam rau kev txav mus los ntawm cov khoom thaum lub sijhawm centrifugation, ua rau muaj cov txheej buffy thiab kev sib cais ntawm cov txheej tsis sib txawv.

5.2 - Kev qeeb ntawm lub tshuab centrifuge:

Txoj kev uas lub centrifuge qeeb qeeb tom qab txoj kev tig kuj tseem tuaj yeem cuam tshuam rau kev sib cais ntshav. Kev qeeb qeeb sai sai yuav cuam tshuam kev faib cov khoom sib xyaw, thaum kev qeeb qeeb maj mam ua rau kev sib cais zoo dua.

Xaus lus:

Xaus lus, kev sib cais ntshav hauv lub centrifuge yog ib qho teeb meem nyuaj uas cuam tshuam los ntawm qhov ceev, qhov loj me, thiab qhov viscoelastic ntawm nws cov khoom. Qhov kev faib ua pawg zoo kawg nkaus ua rau muaj kev sib cais zoo, pab txhawb kev kuaj mob thiab kev kho mob. Kev nkag siab txog qhov nyuaj tom qab cov txheej txheem no ua rau peb txaus siab rau cov ntsiab cai tshawb fawb thiab kev tsim kho uas tsav centrifugation. Raws li thev naus laus zis hloov zuj zus, kev tsim kho tshiab yav tom ntej yuav ua kom zoo dua cov txheej txheem no, thawb cov ciam teb ntawm kev tshawb fawb thiab txhim kho kev kho mob.