Pehea e hoʻokaʻawale ai i ka plasma mai ke koko me ka ʻole o ka centrifuge
Pehea e hoʻokaʻawale ai i ka Plasma mai ke koko me ka ʻole o ka Centrifuge
Hoʻolauna i nā ʻāpana Plasma a me ke Koko
Nā ʻenehana ʻē aʻe no ka hoʻokaʻawale ʻana i ka plasma
Ke Kuleana o nā Mana Kino i ka Hoʻokaʻawale ʻana o ka Plasma
Ke hoʻohana nei i nā ʻano hana Density Gradient no ka hoʻokaʻawale ʻokoʻa
Nā Manaʻo e hiki mai ana a me nā Holomua i nā ʻenehana hoʻokaʻawale Plasma
Hoʻolauna:
ʻO ka plasma, ka ʻāpana wai o ke koko, aia ka nui o nā protein waiwai nui, nā electrolytes, nā hormones, a me nā molekala koʻikoʻi ʻē aʻe. ʻO ka hoʻokaʻawale ʻana i ka plasma mai nā ʻāpana cellular, e like me nā hunaola koko ʻulaʻula a me ke keʻokeʻo, ua hoʻokō maʻamau ʻia me ka hoʻohana ʻana i ka centrifugation. Eia nō naʻe, hiki ke pipiʻi nā centrifuges, koi i kahi kumu mana, a ʻaʻole hiki ke loaʻa mau i nā wahi i kaupalena ʻia i nā kumuwaiwai. Ma kēia ʻatikala, ke ʻimi nei mākou i nā ʻano hana ʻē aʻe e hoʻokaʻawale i ka plasma mai ke koko me ka ʻole o ka hoʻohana ʻana i kahi centrifuge.
Nā ʻAno Hana ʻē aʻe no ka Hoʻokaʻawale ʻana i ka Plasma:
1. Ka hoʻoheheʻe ʻana:
He hana maʻalahi ka Sedimentation e hilinaʻi ana i ka umekaumaha e hoʻokaʻawale i ka plasma mai nā ʻāpana koko. No ka hana ʻana i kēia ʻano hana, huki ʻia ke koko i loko o kahi ʻōmole a waiho ʻia e kū mālie no ka manawa kūpono. I ka wā e noho ana, piholo nā ʻāpana koko ʻulaʻula a me ke keʻokeʻo i lalo ma muli o ko lākou nui o ka paʻa, ʻoiai ʻo ka plasma māmā e noho ana ma luna. Ma ka hōʻiliʻili pono ʻana i ka papa luna, hiki i kekahi ke hoʻokaʻawale i ka plasma mai nā ʻāpana cellular. Eia nō naʻe, hiki ke lōʻihi ka sedimentation ma mua o ka centrifugation a pono ke ahonui.
2. Kānana:
ʻO ke kānana kekahi ʻano hana kūpono no ka hoʻokaʻawale ʻana i ka plasma mai nā ʻāpana koko. Pili kēia ʻano hana i ka hoʻohana ʻana i kahi membrane porous a i ʻole kānana e hopu i nā ʻāpana koko nui aʻe me ka ʻae ʻana i ka plasma e hele. Hiki ke hana ʻia ke kaʻina kānana ma o ke kahe ʻana o ka umekaumaha a i ʻole ma ke kaomi mālie. He mea koʻikoʻi ke koho ʻana i ka mea kānana a me ka nui o ka pore e hōʻoia i ka hoʻokaʻawale plasma maikaʻi me ka ʻole o ka hōʻino ʻana i nā ʻāpana a i ʻole ka nalowale ʻana o nā ʻāpana plasma koʻikoʻi.
Ke Kuleana o nā Mana Kino i ka Hoʻokaʻawale ʻana o ka Plasma:
He kuleana koʻikoʻi ko nā mana kino i ka hoʻokaʻawale ʻana o ka plasma mai nā ʻāpana koko. Pili ka sedimentation a me ka filtration i nā mana umekaumaha a me ka hoʻokaʻawale ʻana ma muli o ka nui. ʻO nā mana kino ʻē aʻe e pili ana e hiki ke kōkua i ka hoʻokaʻawale ʻana, ʻo ia ka mana centrifugal, ke kaomi hydrostatic, a me ka hana capillary. Ma ka hoʻomaopopo ʻana a me ka hoʻopunipuni ʻana i kēia mau mana, ke hoʻomohala mau nei nā mea noiʻi i nā ʻano hana hou no ka hoʻokaʻawale ʻana o ka plasma me ka hilinaʻi ʻole i ka centrifugation.
Ke hoʻohana nei i nā ʻano hana Density Gradient no ka hoʻokaʻawale ʻokoʻa:
Hoʻohana nā ʻano hana gradient density i ke kumumanaʻo o ka centrifugation differential e hoʻokaʻawale i ka plasma mai nā ʻāpana cellular o ke koko. Hoʻomākaukau ʻia kahi medium gradient density ma ke kau ʻana i kahi hopena me nā densities like ʻole. Ke hoʻohui ʻia ke koko ma luna o ka gradient, hoʻoulu ka centrifugation i nā ʻāpana cellular e neʻe i lalo o ka paipu ma muli o ko lākou density. ʻO ka hopena, hana ʻia kahi kaʻawale maopopo, me ka plasma e waiho ana ma luna ma luna o ka gradient. Hāʻawi kēia ʻano hana i ka maʻemaʻe maikaʻi loa a hiki ke hana me ka ʻole o ka centrifuge wikiwiki.
Nā Manaʻo e hiki mai ana a me nā Holomua i nā ʻenehana hoʻokaʻawale Plasma:
Ke hana mau nei nā kānaka noiʻi i ka hoʻomaikaʻi ʻana i nā ʻano hana hoʻokaʻawale plasma me ka hilinaʻi ʻole i ka centrifugation. ʻO kekahi ala e kū mai ana, ʻo ia ka hoʻohana ʻana i nā mea hana microfluidic, e hiki ai ke kaohi pololei i nā wai a me nā ʻāpana i nā nui liʻiliʻi. Ua hoʻolālā ʻia kēia mau kahua miniaturized e hoʻohana i nā ʻano kino like ʻole, e like me ka nui, nā uku uila, a me ka hydrodynamics, e hoʻokaʻawale i ka plasma mai nā ʻāpana koko. Eia kekahi, ʻo ka holomua i ka nanotechnology e alakaʻi i ka hoʻomohala ʻana i nā mea hana nanoscale hou e hiki ke hoʻokaʻawale i ka plasma ma ka pae molekala.
I ka hopena, ʻoiai ʻo ka centrifugation ke kūlana gula no ka hoʻokaʻawale ʻana o ka plasma, ke noiʻi ʻia nei nā ʻano hana ʻē aʻe like ʻole. Hāʻawi kēia mau ʻenehana, e like me ka sedimentation, filtration, a me nā ʻano hana density gradient, i nā koho kūpono no ka hoʻokaʻawale ʻana o ka plasma me ka ʻole o ka pono no ka centrifuge. Ke hoʻomau nei ka holomua ʻana, hiki iā mākou ke manaʻo e hoʻomaikaʻi ʻia ka hiki ke loaʻa i nā ʻenehana hoʻokaʻawale plasma, e wehe ana i nā puka no ka noi ʻana ma nā wahi lapaʻau a me nā wahi i kaupalena ʻia e nā kumuwaiwai.
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