indlela yokuhlukanisa i-plasma negazi ngaphandle kwe-centrifuge

Indlela Yokuhlukanisa I-Plasma Negazi Ngaphandle Kwe-Centrifuge

Isingeniso Sezingxenye Ze-Plasma Negazi

Izindlela Ezihlukile Zokuhlukaniswa Kwe-Plasma

Indima Yamandla Emvelo Ekuhlukanisweni Kwe-Plasma

Ukusebenzisa Izindlela Zobukhulu Bokuhlukaniswa Kokuhlukana

Imibono Yesikhathi Esizayo kanye Nentuthuko Emakhonweni Okuhlukanisa I-Plasma

Isingeniso:

I-Plasma, ingxenye ewuketshezi egazini, iqukethe inqwaba yamaprotheni abalulekile, ama-electrolyte, ama-hormone, namanye ama-molecule abalulekile. Ukuhlukanisa i-plasma nezingxenye zamaseli, njengamaseli egazi abomvu namhlophe, kuye kwafezwa kusetshenziswa i-centrifugation. Kodwa-ke, ama-centrifuge angabiza kakhulu, adinga umthombo wamandla, futhi awatholakali njalo ezindaweni ezilinganiselwe izinsiza. Kulesi sihloko, sihlola ezinye izindlela zokuhlukanisa i-plasma negazi ngaphandle kokusebenzisa i-centrifuge.

Izindlela Ezihlukile Zokuhlukanisa I-Plasma:

1. Ukususwa kwenhlabathi:

Ukususwa kwe-sedimentation kuyindlela elula encike ekudonseleni amandla adonsela phansi ukuze ihlukanise i-plasma kumaseli egazi. Ukuze kwenziwe le ndlela, igazi lidonswa epayipini bese livunyelwa ukuthi lime lingaphazanyiswa isikhathi esanele. Ngesikhathi sokuhlala, amaseli egazi abomvu namhlophe acwila phansi ngenxa yobuningi bawo obuphezulu, kuyilapho i-plasma elula ihlala phezulu. Ngokuqoqa ngokucophelela ungqimba oluphezulu, umuntu angahlukanisa i-plasma nezingxenye zamaseli. Kodwa-ke, ukususwa kwe-sedimentation kungathatha isikhathi eside kakhulu kunokususwa kwe-centrifugation futhi kudinga isineke.

2. Ukuhlunga:

Ukuhlunga kungenye indlela ephumelelayo yokuhlukanisa i-plasma kumaseli egazi. Le ndlela ihilela ukusebenzisa ulwelwesi olunezimbobo noma isihlungi ukuze kubanjwe amaseli egazi amakhulu ngenkathi kuvumela i-plasma ukuthi idlule. Inqubo yokuhlunga ingenziwa ngokugeleza kwamandla adonsela phansi noma ngokusebenzisa ukucindezela okuthambile. Ukukhetha izinto zokuhlunga kanye nosayizi wembobo kubalulekile ukuqinisekisa ukuhlukaniswa kwe-plasma okuphumelelayo ngaphandle kokulimaza amaseli noma ukulahlekelwa yizingxenye ezibalulekile ze-plasma.

Indima Yamandla Emvelo Ekuhlukanisweni Kwe-Plasma:

Amandla angokwenyama adlala indima ebalulekile ekuhlukaniseni i-plasma kumaseli egazi. Kokubili ukuthungwa kanye nokuhlunga kuncike emandleni adonsela phansi kanye nokukhishwa okusekelwe kusayizi, ngokulandelana. Amanye amandla angokwenyama ahlobene angasiza ekuhlukaniseni afaka amandla e-centrifugal, ingcindezi ye-hydrostatic, kanye nesenzo se-capillary. Ngokuqonda nangokulawula la mandla, abacwaningi baqhubeka nokuthuthukisa amasu amasha okuhlukaniswa kwe-plasma ngaphandle kokuthembela ekuhlanganiseni.

Ukusebenzisa Izindlela Zokulinganisa Ubuningi Bokuhlukaniswa Kokuhlukahluka:

Izindlela zokulinganisa ubuningi zisebenzisa isimiso sokuhlukanisa i-plasma ezingxenyeni zegazi. I-density gradient medium ilungiswa ngokubeka isixazululo esinobukhulu obuhlukahlukene. Uma igazi lengezwa ngaphezu kwe-gradient, i-centrifugation ibangela ukuthi izingxenye zeseli zihambe ziye phansi kwepayipi ngokusekelwe ekuqineni kwazo. Ngenxa yalokho, kwenzeka ukuhlukana okucacile, lapho i-plasma ibekwe phezulu ngaphezu kwe-gradient. Le ndlela inikeza ubumsulwa obuhle kakhulu futhi ingenziwa ngaphandle kwe-centrifuge yesivinini esikhulu.

Imibono Yesikhathi Esizayo kanye Nentuthuko Emakhonweni Okuhlukanisa I-Plasma:

Abacwaningi baqhubeka nokusebenza ekuthuthukiseni amasu okuhlukanisa i-plasma ngaphandle kokuthembela ekugxilweni kwe-centrifugation. Enye indlela entsha ukusebenzisa amadivayisi e-microfluidic, avumela ukulawula okunembile uketshezi nezinhlayiya ngamanani amancane. Lawa mapulatifomu amancane aklanyelwe ukusebenzisa izakhiwo ezahlukahlukene zomzimba, njengosayizi, amacala kagesi, kanye ne-hydrodynamics, ukuhlukanisa i-plasma kumaseli egazi. Ngaphezu kwalokho, intuthuko kwezobuchwepheshe be-nano ingaholela ekuthuthukisweni kwamadivayisi e-nanoscale amasha akwazi ukuhlukanisa i-plasma ezingeni lama-molecule.

Ekuphetheni, nakuba i-centrifugation isalokhu iyindinganiso yegolide yokuhlukaniswa kwe-plasma, izindlela ezahlukene ezisetshenziswayo ziyahlolwa. Lezi zindlela, njenge-sedimentation, i-filtration, kanye ne-density gradient methods, zinikeza izinketho ezisebenzayo zokuhlukaniswa kwe-plasma ngaphandle kwesidingo se-centrifuge. Njengoba intuthuko iqhubeka nokuvela, singalindela ukufinyeleleka okuthuthukisiwe kubuchwepheshe bokuhlukanisa i-plasma, okuvula iminyango yokusetshenziswa kokubili ezindaweni zezokwelapha kanye nezinsiza ezilinganiselwe.