indlela yokwahlula iplasma egazini ngaphandle kwe-centrifuge
Indlela Yokwahlula iPlasma kwiGazi ngaphandle kweCentrifuge
Intshayelelo kwiPlasma kunye neeCandelo leGazi
Iindlela Ezizezinye Zokwahlulwa Kweplasma
Indima Yamandla Ezinto Eziphilayo Ekwahlulweni Kweplasma
Ukusebenzisa Iindlela zeGradient yoXinzelelo loKwahlulahlula
Iimbono zexesha elizayo kunye nokuqhubela phambili kwiindlela zokwahlula iPlasma
Intshayelelo:
I-Plasma, inxalenye engamanzi egazini, ineeproteni ezininzi ezixabisekileyo, ii-electrolytes, iihomoni, kunye nezinye iimolekyuli ezibalulekileyo. Ukwahlula i-plasma kwizinto zeseli, ezifana neeseli zegazi ezibomvu nezimhlophe, kuye kwenziwa ngokwesiko ngokusebenzisa i-centrifugation. Nangona kunjalo, ii-centrifuges zinokubiza kakhulu, zifuna umthombo wamandla, kwaye azifumaneki rhoqo kwiindawo ezinqongopheleyo kwezixhobo. Kweli nqaku, sihlola ezinye iindlela zokwahlula i-plasma kwigazi ngaphandle kokusebenzisa i-centrifuge.
Iindlela Ezizezinye Zokwahlulwa Kweplasma:
1. Ukutshatyalaliswa kwenkunkuma:
Ukusasazwa kweseli yindlela elula exhomekeke kumxhuzulane ukuze yahlule iplasma kwiiseli zegazi. Ukuze kwenziwe le ndlela, igazi litsalwa kwityhubhu kwaye livunyelwe ukuba lime lingaphazanyiswa ixesha elaneleyo. Ngexesha lokuhlala, iiseli zegazi ezibomvu nezimhlophe ziyatshona ezantsi ngenxa yoxinano lwazo oluphezulu, ngelixa iplasma ekhaphukhaphu ihlala phezulu. Ngokuqokelela ngononophelo umaleko ophezulu, umntu unokwahlula iplasma kwiinxalenye zeseli. Nangona kunjalo, ukusabalala kweseli kungathatha ixesha elide kakhulu kune-centrifugation kwaye kufuna umonde.
2. Ukuhluza:
Ukucoca yenye indlela esebenzayo yokwahlula i-plasma kwiiseli zegazi. Le ndlela ibandakanya ukusebenzisa i-membrane enemingxuma okanye isihluzi ukubamba iiseli zegazi ezinkulu ngelixa ivumela i-plasma ukuba idlule. Inkqubo yokucoca inokwenziwa ngokuhamba komxhuzulane okanye ngokusebenzisa uxinzelelo oluthambileyo. Ukukhetha izinto zokucoca kunye nobukhulu bemingxuma kubalulekile ukuqinisekisa ukwahlukana kwe-plasma ngokufanelekileyo ngaphandle kokonakalisa iiseli okanye ukulahlekelwa ziinxalenye ezibalulekileyo ze-plasma.
Indima Yamandla Ezinto Eziphilayo Ekwahlulweni Kweplasma:
Amandla endalo adlala indima ebalulekileyo ekwahlulweni kweplasma kwiiseli zegazi. Zombini i-sedimentation kunye ne-filtration zixhomekeke kumandla adonsela phantsi kunye nokukhutshwa okusekwe kubungakanani, ngokulandelelana. Amanye amandla endalo anxulumene nawo anokunceda ukwahlukana aquka amandla e-centrifugal, uxinzelelo lwe-hydrostatic, kunye ne-capillary action. Ngokuqonda kunye nokulawula la mandla, abaphandi bahlala bephuhlisa iindlela ezintsha zokwahlula i-plasma ngaphandle kokuxhomekeka kwi-centrifugation.
Ukusebenzisa Iindlela ze-Density Gradient zoKwahlulahlula umahluko:
Iindlela zobuninzi be-density gradient zisebenzisa umgaqo we-differential centrifugation ukwahlula i-plasma kwi-cellular components yegazi. I-density gradient medium ilungiswa ngokudibanisa isisombululo esine-densities ezahlukeneyo. Xa igazi longezwa ngaphezulu kwe-gradient, i-centrifugation ibangela ukuba ii-cellular components zihambe ziye ezantsi kwetyhubhu ngokusekelwe kubuninzi bazo. Ngenxa yoko, ukwahlukana okucacileyo kwenzeka, apho i-plasma ibekwe phezulu ngaphezu kwe-gradient. Le ndlela inika ubunyulu obuhle kakhulu kwaye inokwenziwa ngaphandle kwe-high-speed centrifuge.
Iimbono zexesha elizayo kunye nokuqhubela phambili kwiindlela zokwahlulahlula iplasma:
Abaphandi baqhubeka besebenza ekuphuculeni iindlela zokwahlulahlula i-plasma ngaphandle kokuxhomekeka kwi-centrifugation. Enye indlela entsha kukusebenzisa izixhobo ze-microfluidic, ezivumela ulawulo oluchanekileyo phezu kolwelo kunye namasuntswana ngamanani amancinci. La maqonga amancinci ayilwe ukuze asebenzise iipropati ezahlukeneyo zomzimba, ezinje ngobukhulu, iindleko zombane, kunye ne-hydrodynamics, ukwahlula i-plasma kwiiseli zegazi. Ngaphezu koko, ukuqhubela phambili kwi-nanotechnology kunokukhokelela ekuphuhlisweni kwezixhobo ezintsha ze-nanoscale ezikwaziyo ukwahlula i-plasma kwinqanaba le-molecular.
Ukuqukumbela, nangona i-centrifugation isengumgangatho ophambili wokwahlulwa kwe-plasma, iindlela ezahlukeneyo ezizezinye ziyahlolwa. Ezi ndlela, ezinje nge-sedimentation, i-filtration, kunye neendlela ze-density gradient, zibonelela ngeendlela ezifanelekileyo zokwahlulwa kwe-plasma ngaphandle kwesidingo se-centrifuge. Njengoko uphuhliso luqhubeka luvela, sinokulindela ukufikeleleka okuphuculweyo kwitekhnoloji yokwahlulwa kwe-plasma, okuvula iingcango zokusetyenziswa kuzo zombini iimeko zeklinikhi kunye nezibonelelo ezinqongopheleyo.
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