mokhoa oa ho arola plasma ho mali ntle le centrifuge

Mokhoa oa ho Arohanya Plasma le Mali ntle le Centrifuge

Selelekela sa Plasma le Likarolo tsa Mali

Mekhoa e Meng ea ho Aroloa ha Plasma

Karolo ea Matla a 'Mele Karohanong ea Plasma

Ho Sebelisa Mekhoa ea Gradient ea Botenya bakeng sa Karohano e Fapaneng

Maikutlo a Nakong e Tlang le Tsoelo-pele Mekhoeng ea Karohano ea Plasma

Selelekela:

Plasma, karolo e metsi ea mali, e na le liprotheine tse ngata tsa bohlokoa, li-electrolyte, lihormone le limolek'hule tse ling tsa bohlokoa. Ho arola plasma ho likarolo tsa sele, tse kang lisele tse khubelu le tse tšoeu tsa mali, ka tloaelo ho 'nile ha finyelloa ka ho sebelisa centrifugation. Leha ho le joalo, centrifuge e ka ba theko e boima, ea hloka mohloli oa motlakase, 'me ha se kamehla e fumanehang libakeng tse fokolang tsa mehloli. Sehloohong sena, re hlahloba mekhoa e meng ea ho arola plasma ho mali ntle le ho sebelisa centrifuge.

Mekhoa e Meng ea ho Arohanya Lero la Mali:

1. Ho senngoa ha lerōle:

Ho ntsha sedimentation ke mokhoa o bonolo o itshetlehileng hodima matla a khoheli ho arola plasma ho disele tsa madi. Ho etsa mokgwa ona, madi a hulwa ka hara tube mme a dumellwa ho ema a sa sitiswe ka nako e lekaneng. Nakong ya ho dula, disele tse kgubedu le tse tshweu tsa madi di teba tlase ka lebaka la bongata ba tsona bo phahameng, ha plasma e bobebe e ntse e le ka hodimo. Ka ho bokella lera le ka hodimo ka hloko, motho a ka arola plasma ho disele. Leha ho le jwalo, ho ntsha sedimentation ho ka nka nako e telele haholo ho feta ho ntsha sedimentation mme ho hloka mamello.

2. Ho sefa:

Ho sefa ke mokhoa o mong o sebetsang oa ho arola plasma ho lisele tsa mali. Mokhoa ona o kenyelletsa ho sebelisa lera le nang le masoba kapa sefe ho tšoara lisele tse kholo tsa mali ha ka nako e ts'oanang ho lumella plasma ho feta. Ts'ebetso ea ho sefa e ka etsoa ka phallo ea matla a khoheli kapa ka ho sebelisa khatello e bonolo. Khetho ea thepa ea sefe le boholo ba masoba li bohlokoa ho netefatsa karohano e sebetsang ea plasma ntle le ho senya lisele kapa ho lahleheloa ke likarolo tsa bohlokoa tsa plasma.

Karolo ea Matla a 'Mele Karohanong ea Plasma:

Matla a 'mele a phetha karolo ea bohlokoa karohanong ea plasma ho lisele tsa mali. Ho senngoa ha sedimentation le ho sefshoa ka bobeli ho itšetlehile ka matla a khoheli le ho tlosoa ho thehiloeng boholong, ka ho latellana. Matla a mang a 'mele a amanang le 'mele a ka thusang karohano a kenyelletsa matla a centrifugal, khatello ea hydrostatic, le ketso ea capillary. Ka ho utloisisa le ho laola matla ana, bafuputsi ba ntse ba tsoela pele ho nts'etsapele mekhoa e mecha ea karohano ea plasma ntle le ho itšetleha ka centrifugation.

Ho Sebelisa Mekhoa ea Gradient ea Botenya bakeng sa Karohano e Fapaneng:

Mekhoa ea gradient ea density e sebelisa molao-motheo oa differential centrifugation ho arola plasma ho tsoa ho likarolo tsa sele tsa mali. Medium ea gradient ea density e lokisoa ka ho beha tharollo ka bongata bo fapaneng. Ha mali a eketsoa ka holim'a gradient, centrifugation e etsa hore likarolo tsa sele li tsamaee ho ea tlase ho tube ho latela bongata ba tsona. Ka lebaka leo, ho hlaha karohano e hlakileng, 'me plasma e lutse holimo ka holim'a gradient. Mokhoa ona o fana ka bohloeki bo botle 'me o ka etsoa ntle le centrifuge ea lebelo le phahameng.

Maikutlo a Nakong e Tlang le Tsoelo-pele Mekhoeng ea Karohano ea Plasma:

Bafuputsi ba ntse ba sebetsa ka thata ho ntlafatsa mekhoa ea karohano ea plasma ntle le ho itšetleha ka centrifugation. Mokhoa o mong o hlahang ke tšebeliso ea lisebelisoa tsa microfluidic, tse nolofalletsang taolo e nepahetseng holim'a metsi le likaroloana ka bongata bo bonyenyane. Li-platform tsena tse nyane li etselitsoe ho sebelisa thepa e fapaneng ea 'mele, joalo ka boholo, litefiso tsa motlakase le hydrodynamics, ho arola plasma ho lisele tsa mali. Ho feta moo, tsoelo-pele ea nanotechnology e ka lebisa nts'etsopele ea lisebelisoa tse ncha tsa nanoscale tse khonang ho arola plasma boemong ba limolek'hule.

Qetellong, leha ho centrifugation e ntse e le tekanyetso ea khauta bakeng sa karohano ea plasma, mekhoa e meng e fapaneng e ntse e hlahlojoa. Mekhoa ena, joalo ka sedimentation, sefiltration, le mekhoa ea density gradient, e fana ka likhetho tse sebetsang bakeng sa karohano ea plasma ntle le tlhoko ea centrifuge. Ha tsoelo-pele e ntse e tsoela pele ho hlaha, re ka lebella phihlello e ntlafetseng ea mahlale a karohano ea plasma, e bulang mamati a ts'ebeliso maemong a bongaka le a fokolang a mehloli.