Ukukhiqizwa kobungcweti kwesihlukanisi se-centrifuge kanye nomkhiqizi wemishini yemithi - i-Zonelink
kungani kwenziwa i-centrifuge yegazi？ i-centrifuge| I-ZONELINK
Njengoba sonke sazi, ukuhlukaniswa kwe-serum kuyinkinga enzima, ngakho-ke ungayisebenzisa kanjani i-centrifuge ukuhlukaniswa kwe-serum? Okokuqala, sihlaziya kusukela emqondweni we-serum:
i-serum yegazi: Uketshezi oluhlukanisa igazi ngemva kokujiya.
Ingqikithi yokujiya kwegazi ukuguqulwa kwe-fibrinogen encibilikayo ibe yi-fibrin engancibiliki ku-plasma. Amaprotheni ane-fibrous ayizinhlayiya ezincane ezihlangana zibe amaseli egazi, zakha ama-clots afana ne-gel. Ngemva kwemizuzu engama-30 kuya kwehora eli-1 igazi lijiyile, i-blood clot ku-platelet systolic protein contraction, ukuze i-clot ibuye iqine, i-extrusion clear liquid, eyaziwa ngokuthi i-serum. I-serum ihlukile ku-plasma ngoba ayinayo i-fibrinogen kanye nezinto ezijiyile ezihilelekile ekujiyileni kwegazi, kodwa ingeze inani elincane lezinto ezikhishwa ama-platelet ngesikhathi sokujiyile.
Ngakho ake ngibuyele enjongweni yokuhlukaniswa kwe-serum
Ulwazi oluvamile:
Yima endaweni yokushisa yegumbi (ama-degree angu-37) isikhathi esingaphezu kwesigamu sehora, vula i-centrifuge, bese u-centrifuge ku-3500-4000rpm imizuzu emi-5-10. I-serum ihlukanisiwe. Uma ama-enzyme okulinganisa nezinye izinkomba kulula ukuziqeda, kuye ngesidingo sokufaka ama-degree angu-4 isikhathi esingaphezu kwesigamu sehora, centrifuge. (Inhloso yokuyishiya kuma-degree angu-37 isikhashana ukuvumela igazi ukuthi lijiye futhi likhulule i-serum, ngoba lapho igazi lijiya, i-fibrin iyafinyela bese i-serum iyaphuma.)
Izindaba ezidinga ukunakwa
1 Ijubane le-centrifugal likhulu kakhulu, kulula ukulibangela i-hemolysis
2 Igazi lezilwane, zama ukunganamatheli ezinweleni, kulula ukulisusa.
Amathiphu: Ngemva kokususwa kwegazi, ungabeki isitsha siqonde, sicaba noma sitsheke, ukuze i-serum ikwazi ukwandisa indawo yemvula, okuzokwenza kube lula ukuna kwe-serum.
Ngamafuphi, inkinga eyinhloko ukuqoqa igazi kahle, ukubamba isikhathi sokuma kwezinga lokushisa legumbi, bese usebenzisa i-centrifuge ekhethekile yokuhlukanisa i-serum, ukuze ugweme i-hemolysis.
