se arotsoeng ka har'a centrifuge

Ke eng e arohantsoeng ka har'a Centrifuge?

Selelekela:

Li-centrifuge li sebelisoa haholo indastering e fapaneng, ho kenyeletsoa le lipatlisiso tsa meriana, lik'hemik'hale le tsa baeloji. Mechini ena e matla e sebelisa matla a centrifugal ho arola likarolo tse fapaneng tsa motsoako ho latela bongata ba tsona. Sehloohong sena, re tla teba haholoanyane ts'ebetsong ea centrifuge le ho hlahloba lintho tse fapaneng tse ka aroloang ka katleho ho sebelisoa mokhoa ona.

I. Ho utloisisa Centrifugation:

A. Molao-motheo oa centrifugation

B. Likarolo tsa bohlokoa tsa centrifuge

C. Mefuta ea li-centrifuge

Ho centrifugation ke mokhoa o sebelisang ho potoloha ka lebelo le phahameng ho hlahisa matla a centrifugal, a fetang matla a khoheli ka makhetlo a likete tse 'maloa. Matla ana a etsa hore likaroloana kapa likarolo tse teteaneng motsoakong li lule kapa li arohane le tse sa teteaneng haholo. Ts'ebetso eohle e etsahala ka har'a sesebelisoa se ikhethileng se tsejoang e le centrifuge.

Likarolo tsa motheo tsa centrifuge li kenyelletsa rotor, e tšoereng disampole, le enjene e potolohang rotor ka lebelo le phahameng. Mefuta e fapaneng ea centrifuge e fumaneha ka bokhoni bo fapaneng, meralo ea rotor, le lebelo le phahameng ka ho fetisisa. Mefuta e meng e tloaelehileng e kenyelletsa microcentrifuge e sebelisoang bakeng sa libolumo tse nyane tsa sampole, ultracentrifuge e khonang ho etsa lebelo le matla a holimo, le centrifuge ea deske e lebelo le phahameng e atisang ho fumanoa lilaboratoring tsa bongaka.

II. Ho Arola Likarolo tsa Selefouno:

A. Lisele tse khubelu tsa mali le plasma

B. Kobo e nang le boea bo teteaneng: Lera le bohareng

C. Dikopo bankeng ea mali

E 'ngoe ea lits'ebetso tse tloaelehileng tsa ho centrifugation ke ho arola likarolo tsa sele tsa mali. Ha sampole ea mali e kenngoa tlas'a matla a centrifugal, ho thehoa likarolo tse tharo tse fapaneng. Lisele tse khubelu tse boima li lula tlase, plasma e bobebe e phaphamala holimo, 'me pakeng tsa tsona, ho thehoa lera le lesesaane le lesoeu le bohareng le bitsoang buffy coat.

Mokhoa ona o sebelisoa haholo libankeng tsa mali le lilaboratoring tsa bongaka. Ka ho arola likarolo tse fapaneng tsa sele ka katleho, li-centrifuge li nolofalletsa ho arola le ho hlahloba plasma, lisele tse khubelu tsa mali le boea bo teteaneng. Sena se lumella ho lemoha mafu a fapaneng, a kang khaello ea mali, leukemia le tšoaetso.

III. Karohano ka Gradient ea Botenya:

A. Selelekela ho centrifugation ea gradient ea bongata

B. Ho arola DNA le RNA

C. Karohano ea li-organelle

Ha ho centrifugation ea gradient ea density, density gradient medium e eketsoa liphaepheng tsa centrifuge. Medium ena e etsa gradient ea mahloriso, ho tloha ho density e phahameng tlase ho ea ho density e tlase holimo. Ha motsoako o centrifugation boteng ba gradient ea density, likarolo tsa motsoako li lula libakeng tse fapaneng ho latela bongata ba tsona, e leng se lumellang karohano e sebetsang hantle.

Mokhoa ona o sebelisoa haholo baelojing ea limolek'hule le biochemistry. O nolofalletsa karohano ea limolek'hule tsa DNA le RNA ho latela boholo le bongata ba tsona, e leng ntho ea bohlokoa bakeng sa ho ithuta lintho tsa lefutso. Ho potoloha ha gradient ea density ho boetse ho sebelisoa ho arola lisele, e leng mokhoa o arolang li-organelle tse fapaneng, tse kang mitochondria, ho tsoa ho li-homogenate tsa sele.

IV. Ho Arola Likaroloana tsa Boholo bo Fapaneng:

A. Ho potoloha ha lebelo la sedimentation

B. Ho arola li-nanoparticles

C. Ditshebediso dipatlisisong tsa nanoparticle

Sedimentation velocity centrifugation e sebediswa ha sepheo e le ho arola dikarolwana tsa boholo bo fapaneng ka hara motswako. Ha centrifuge e ntse e potoloha ka potlako, dikarolwana tse nyane di dula butle ka lebaka la boima ba tsona bo tlase, e leng se fellang ka ho thehwa ha dihlopha kapa dikarolwana tse fapaneng ka hara sampole.

Mokhoa ona o bohlokoa haholo lipatlisisong tsa nanoparticle, moo karohano e nepahetseng le tlhaloso ea nanoparticles li leng bohlokoa. Ka ho fetola lebelo le bolelele ba nako ea centrifugation, bo-rasaense ba ka arola nanoparticles ho latela boholo ba tsona, e leng se ba nolofalletsang ho ithuta thepa ea tsona, botsitso le ts'ebeliso mafapheng a kang bongaka, saense ea tikoloho le lisebelisoa tsa elektroniki.

V. Karohano ea Lipula:

A. Ho tlosa thepa e sa qhibiliheng

B. Karolo lits'ebetsong tsa tlhoekiso

C. Likopo tsa indasteri

Ho centrifugation ho boetse ho sebediswa ho arola di-precipitate ho tswa ho motswako wa metsi. Di-precipitate ke dikarolwana tse tiileng tse qetellang di dula tlase ha ho etsahala karabelo e itseng ya dikhemikhale kapa ha dintho tse itseng di eketswa ka hara tharollo. Ka ho kenya motswako ho centrifugation, di-precipitate di ka arolwa ka katleho le mokelikeli o matla.

Karolo ena ea ho centrifugation e sebelisoa haholo lits'ebetsong tsa tlhoekiso ka har'a lik'hemik'hale, meriana le indasteri ea lijo. E lumella ho tlosoa ha lintho tse tiileng tse sa batleheng, litšila le litšila tse tsoang metsing, e leng se lebisang boleng bo holimo ba sehlahisoa le bohloeki.

Qetello:

Li-centrifuge li bapala karolo ea bohlokoa karohanong ea lintho tse fapaneng ho latela bongata ba tsona, boholo, kapa ho se qhibilihe ha tsona. Ho tloha likarolong tsa mali ho ea ho li-nanoparticles, mekhoa ea ho centrifuge e sebelisoa haholo liindastering tse fapaneng le lipatlisisong tsa mahlale ho fumana likaroloana tse hloekisitsoeng haholo le ho arola likarolo tse itseng tse khahlisang. Ho utloisisa melao-motheo le ts'ebeliso ea centrifuge ho bohlokoa bakeng sa bo-rasaense, litsebi tsa theknoloji le batho ka bomong ba sebetsang masimong a mangata moo karohano e sebetsang e leng ea bohlokoa.