he aha te mea ka wehea i roto i te centrifuge

He aha te mea e wehea ana i roto i te centrifuge?

Kupu Whakataki:

He whānuitia te whakamahinga o ngā pūwero i roto i ngā ahumahi maha, tae atu ki te rangahau rongoā, matū, me te koiora. Ka whakamahia e ēnei mīhini kaha te kaha pūwero hei wehewehe i ngā wāhanga rerekē o te ranunga i runga i tō rātou mātotoru. I roto i tēnei tuhinga, ka ruku hohonu ake tātou ki ngā mahi a te pūwero, ā, ka tūhuratia ngā matū rerekē ka taea te wehewehe pai mā te whakamahi i tēnei tikanga.

I. Te Mārama ki te Whakapūngao:

A. Te mātāpono o te centrifugation

B. Ngā wāhanga matua o te mīhini centrifuge

C. Ngā momo pūwhakapūmau

Ko te centrifugation he tikanga e whakamahi ana i te hurihanga tere-teitei hei whakaputa i te kaha centrifuge, he mano ngā wā e nui ake ana i te kaha o te kaha ā-papa. Mā tēnei kaha ka noho, ka wehe rānei ngā matūriki, ngā wāhanga rānei o te ranunga kia matotoru ake i ngā mea iti ake te matotoru. Ka puta te tukanga katoa i roto i tētahi taputapu motuhake e mōhiotia ana ko te centrifuge.

Ko ngā wāhanga matua o te centrifuge ko te rotor, e pupuri ana i ngā tauira, me te motuka e huri ana i te rotor i ngā tere teitei. He rerekē ngā momo centrifuge e wātea ana me ngā kaha rerekē, ngā hoahoa rotor, me ngā tere mōrahi. Ko ētahi momo noa ko ngā microcentrifuge e whakamahia ana mō ngā rahinga tauira iti, ngā ultracentrifuge e kaha ana ki te tere me te kaha teitei ake, me ngā centrifuge tēpu tere-teitei e kitea ana i roto i ngā taiwhanga hauora.

II. Te Wehewehe i ngā Wāhanga Pūtau:

A. Ngā pūtau toto whero me te plasma

B. Koti pūhuruhuru: Te paparanga waenga

C. Ngā tono i roto i te peeke toto

Ko tētahi o ngā whakamahinga tino noa o te centrifugation ko te wehewehe i ngā wāhanga pūtau o te toto. Ina tukuna he toto ki te kaha centrifugal, ka hangaia he paparanga motuhake e toru. Ko ngā toto toto whero taumaha ka tau ki raro, ko ngā toto toto māmā ka mānu ki runga, ā, i waenganui i a rātou, ka hangaia he paparanga waenga angiangi, mā e kiia nei ko te buffy coat.

He nui te whakamahinga o tēnei tikanga i roto i ngā whare pupuri toto me ngā taiwhanga haumanu. Mā te wehewehe pai i ngā wāhanga pūtau rerekē, ka taea e ngā centrifuge te wehe me te whakamātautau i muri mai i te plasma, ngā toto whero, me te huruhuru buffy. Mā tēnei ka taea te kite i ngā momo mate, pērā i te anemia, te leukemia, me ngā mate pāwera.

III. Te Wehewehenga mā te Rōrahi Matotoru:

A. Whakataki ki te whakapūtanga o te pikinga mātotoru

B. Te wehewehe i te DNA me te RNA

C. Te wehewehenga o ngā whekau

I roto i te whakapūtanga pikinga mātotoru, ka tāpirihia he reo pikinga mātotoru ki ngā ngongo whakapūtanga. Ka hangaia e tēnei reo he pikinga kukū, mai i te kiato teitei ake i raro ki te kiato iti iho i runga. Ina whakapūtangahia he ranunga i te aroaro o te pikinga mātotoru, ka tau ngā wāhanga o te ranunga ki ngā wāhi rerekē i runga i ō rātou kiato, ka taea ai te wehewehe pai.

He whānuitia te whakamahinga o tēnei tikanga i roto i te koiora ngota me te matū koiora. Mā tēnei ka taea te wehewehe i ngā ngota DNA me te RNA i runga i ō rātou rahi me te kiato, he mea nui tēnei mō te ako i ngā rauemi ira. Ka whakamahia hoki te centrifugation gradient kiato i roto i te wehewehenga pūtau, he tukanga e wehe ana i ngā momo whekau, pērā i ngā mitochondria, mai i ngā homogenates pūtau.

IV. Te Wehewehe i ngā Matūriki o ngā Rahi Rerekē:

A. Te tere o te whakapūpūtanga o te oneone

B. Te wehewehe i ngā matūriki nano

C. Ngā tono i roto i te rangahau matūriki nano

Ka whakamahia te whakateretere tere o te paru ina ko te whāinga he wehewehe i ngā matūriki o ngā rahi rerekē i roto i te ranunga. I te tere o te hurihuri o te whakateretere, ka pōturi ake te taunga o ngā matūriki iti nā te iti o tō rātou taumaha, ka hua ake te hanganga o ngā roopu motuhake, ngā wāhanga rānei i roto i te tauira.

He mea nui tēnei tikanga i roto i te rangahau matūriki nano, he mea nui te wehewehe me te tautuhi tika i ngā matūriki nano. Mā te whakarerekē i te tere me te roa o te centrifugation, ka taea e ngā kaipūtaiao te wehewehe i ngā matūriki nano i runga i tō rātou rahi, ka taea ai e rātou te ako i ō rātou āhuatanga, te pumau, me ngā tono i roto i ngā mara pēnei i te rongoā, te pūtaiao taiao, me te hikohiko.

V. Te Wehenga o ngā Uaua:

A. Te tango i ngā rauemi kore-rewa

B. Te tūranga i roto i ngā tukanga purenga

C. Ngā tono ahumahi

Ka whakamahia hoki te centrifugation hei wehe i ngā matūriki mai i te ranunga wai. Ko ngā matūriki he matūriki totoka ka tau ki raro ina puta he tauhohenga matū, ina tāpirihia rānei ētahi matū ki te otinga. Mā te tuku i te ranunga ki te centrifugation, ka taea te wehe pai i ngā matūriki mai i te wai i runga ake.

He whānuitia te whakamahinga o tēnei āhuatanga o te centrifugation i roto i ngā tukanga purenga i roto i ngā ahumahi matū, rongoā, me te kai. Mā tēnei ka taea te tango i ngā totoka, ngā poke, me ngā poke kāore e hiahiatia mai i ngā wai, ka hua ake te kounga me te parakore o te hua.

Whakatau:

He mea nui te mahi a ngā pūwhakapū i roto i te wehewehe i ngā matū rerekē i runga i te kiato, te rahi, te kore rānei e wairewa. Mai i ngā wāhanga toto ki ngā matūriki nano, e whakamahia whānuitia ana ngā tikanga whakapū i roto i ngā ahumahi me ngā rangahau pūtaiao rerekē hei whiwhi i ngā wāhanga tino parakore me te wehe i ngā wāhanga motuhake e hiahiatia ana. He mea nui te mārama ki ngā mātāpono me ngā tono o te whakapū mō ngā kaipūtaiao, ngā tohunga hangarau, me ngā tāngata takitahi e mahi ana i ngā mara maha e matua ana te wehewehenga whai hua.