dab tsi yog sib cais hauv centrifuge
Dab tsi raug cais tawm hauv Centrifuge?
Kev Taw Qhia:
Cov tshuab centrifuges siv dav hauv ntau yam lag luam, suav nrog kev tshawb fawb txog tshuaj, tshuaj lom neeg, thiab kev tshawb fawb txog tsiaj txhu. Cov tshuab muaj zog no siv lub zog centrifugal los cais cov khoom sib xyaw ua ke raws li lawv qhov ceev. Hauv tsab xov xwm no, peb yuav kawm tob txog kev ua haujlwm ntawm lub tshuab centrifuge thiab tshawb nrhiav ntau yam tshuaj uas tuaj yeem cais tau zoo siv cov txheej txheem no.
I. Kev Nkag Siab Txog Kev Siv Centrifugation:
A. Lub hauv paus ntsiab lus ntawm centrifugation
B. Cov khoom tseem ceeb ntawm lub centrifuge
C. Hom centrifuges
Kev centrifugation yog ib txoj kev siv lub zog tig ceev ceev los tsim lub zog centrifugal, uas yog ntau txhiab npaug ntau dua li lub zog ntawm lub ntiajteb txawj nqus. Lub zog no ua rau cov khoom me me lossis cov khoom sib xyaw ua ke hauv cov khoom sib xyaw kom sib haum lossis sib cais ntawm cov uas tsis ntom ntom. Tag nrho cov txheej txheem tshwm sim hauv ib lub cuab yeej tshwj xeeb hu ua centrifuge.
Cov khoom tseem ceeb ntawm lub centrifuge muaj xws li lub rotor, uas tuav cov qauv, thiab lub cev muaj zog uas tig lub rotor ntawm qhov ceev siab. Muaj ntau hom centrifuges sib txawv nrog ntau lub peev xwm, cov qauv rotor, thiab qhov ceev siab tshaj plaws. Qee hom feem ntau suav nrog microcentrifuges siv rau cov qauv me me, ultracentrifuges uas muaj peev xwm ceev thiab muaj peev xwm siab dua, thiab cov centrifuges ceev ceev uas feem ntau pom hauv cov chaw kuaj mob.
II. Kev Cais Cov Cheebtsam Cellular:
A. Cov qe ntshav liab thiab cov ntshav hauv lub cev
B. Lub tsho tiv no: Lub txheej nruab nrab
C. Cov ntawv thov hauv kev khaws cov ntshav
Ib qho ntawm cov kev siv centrifugation feem ntau yog kev cais cov khoom hauv lub cev ntawm cov ntshav. Thaum cov ntshav kuaj raug rau lub zog centrifugal, peb txheej sib txawv raug tsim. Cov qe ntshav liab hnyav dua nyob hauv qab, cov plasma sib dua ntab saum toj, thiab ntawm lawv, ib txheej nruab nrab dawb nyias nyias hu ua buffy coat raug tsim.
Cov txheej txheem no pom tias muaj kev siv ntau hauv kev khaws cov ntshav thiab cov chaw kuaj mob hauv tsev kho mob. Los ntawm kev sib cais cov khoom sib txawv ntawm cov cellular, centrifuges ua rau muaj kev sib cais thiab kev kuaj tom qab ntawm cov plasma, cov qe ntshav liab, thiab cov plaub mos mos. Qhov no tso cai rau kev kuaj pom ntau yam kab mob, xws li ntshav tsis txaus, mob leukemia, thiab kev kis kab mob.
III. Kev sib cais los ntawm qhov ceev Gradient:
A. Kev Taw Qhia Txog Kev Siv Lub Tshuab Centrifugation Uas Muaj Qhov Ceev
B. Kev cais DNA thiab RNA
C. Kev faib ua feem ntawm cov organelles
Hauv kev centrifugation uas muaj qhov ceev sib txawv, ib qho nruab nrab ntawm qhov ceev sib txawv raug ntxiv rau hauv cov raj centrifuge. Cov nruab nrab no tsim ib qho kev sib txawv ntawm qhov ceev sib txawv, xws li qhov ceev siab dua ntawm qab mus rau qhov ceev qis dua ntawm sab saum toj. Thaum ib qho khoom sib tov raug centrifuged nyob rau hauv qhov muaj qhov ceev sib txawv, cov khoom sib xyaw ntawm cov khoom sib tov yuav nyob rau ntawm ntau qhov sib txawv raws li lawv qhov ceev, ua rau muaj kev sib cais zoo.
Cov txheej txheem no siv dav hauv kev tshawb fawb txog molecular biology thiab biochemistry. Nws ua rau muaj kev sib cais ntawm DNA thiab RNA molecules raws li lawv qhov loj me thiab qhov ceev, uas yog qhov tseem ceeb rau kev kawm txog cov khoom siv caj ces. Kev siv density gradient centrifugation kuj tseem siv rau hauv kev faib ua feem ntawm lub hlwb, ib qho txheej txheem uas cais ntau yam organelles, xws li mitochondria, los ntawm cov homogenates ntawm lub hlwb.
IV. Kev Cais Cov Khoom Me Me Txawv Txawv:
A. Kev centrifugation ceev ntawm cov av noo
B. Kev cais cov nanoparticles
C. Cov ntawv thov hauv kev tshawb fawb nanoparticle
Kev siv lub tshuab centrifugation uas ceev thaum cov av poob yog siv thaum lub hom phiaj yog cais cov khoom me me uas muaj qhov loj sib txawv hauv cov khoom sib tov. Thaum lub tshuab centrifuge tig sai sai, cov khoom me me yuav poob qeeb dua vim lawv qhov hnyav tsawg dua, ua rau muaj cov kab sib txawv lossis cov feem sib txawv hauv cov qauv.
Cov txheej txheem no tseem ceeb heev rau kev tshawb fawb txog nanoparticle, qhov twg kev sib cais thiab kev piav qhia meej ntawm cov nanoparticles yog qhov tseem ceeb. Los ntawm kev hloov pauv qhov ceev thiab lub sijhawm ntawm centrifugation, cov kws tshawb fawb tuaj yeem sib cais cov nanoparticles raws li lawv qhov loj me, ua rau lawv kawm txog lawv cov khoom, kev ruaj khov, thiab kev siv hauv cov teb xws li tshuaj, kev tshawb fawb ib puag ncig, thiab hluav taws xob.
V. Kev Sib Cais Cov Nag Los:
A. Tshem tawm cov khoom uas tsis yaj
B. Lub luag haujlwm hauv cov txheej txheem ntxuav
C. Cov ntawv thov kev lag luam
Kev siv centrifugation kuj siv los cais cov precipitates ntawm cov kua sib tov. Cov precipitates yog cov khoom khov uas thaum kawg poob rau hauv qab thaum muaj kev tshuaj lom neeg tshwj xeeb tshwm sim lossis thaum muaj qee yam tshuaj ntxiv rau hauv cov tshuaj. Los ntawm kev muab cov khoom sib tov rau centrifugation, cov precipitates tuaj yeem cais tau zoo ntawm cov kua supernatant.
Qhov no ntawm centrifugation pom muaj kev siv dav hauv cov txheej txheem ntxuav hauv cov tshuaj lom neeg, tshuaj, thiab zaub mov. Nws tso cai rau kev tshem tawm cov khoom tsis xav tau, cov khoom tsis huv, thiab cov khoom tsis huv ntawm cov kua, ua rau cov khoom zoo dua thiab huv si.
Xaus lus:
Cov centrifuges ua lub luag haujlwm tseem ceeb hauv kev sib cais ntawm ntau yam khoom raws li lawv qhov ceev, qhov loj me, lossis qhov tsis yaj. Txij li cov khoom ntshav mus rau nanoparticles, cov txheej txheem centrifugation yog siv dav hauv ntau yam lag luam thiab kev tshawb fawb kom tau txais cov feem pua huv heev thiab cais cov khoom tshwj xeeb uas xav tau. Kev nkag siab txog cov ntsiab cai thiab kev siv ntawm centrifugation yog qhov tseem ceeb rau cov kws tshawb fawb, cov kws tshaj lij, thiab cov tib neeg ua haujlwm thoob plaws ntau qhov chaw uas kev sib cais zoo yog qhov tseem ceeb.
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