Kodi zosakaniza zimalekanitsidwa bwanji mkati mwa centrifuge

Chiyambi cha Centrifugation

Kutsegula magetsi ndi njira yomwe imagwiritsidwa ntchito kwambiri m'magawo osiyanasiyana asayansi, azachipatala, ndi mafakitale polekanitsa zosakaniza kutengera kuchuluka kwake. Njira yothandizayi imaphatikizapo kugwiritsa ntchito makina otsegula magetsi, omwe amagwiritsa ntchito mphamvu ya centrifugal kulekanitsa zigawo za zosakaniza. Kwa zaka zambiri, kutsegula magetsi kwakhala chida chofunikira kwambiri m'ma laboratories ofufuza, malo oyezera matenda, ndi m'malo opangira mafakitale chifukwa cha kuthekera kwake kutulutsa zinthu ndi tinthu tating'onoting'ono kuchokera ku zosakaniza zovuta. M'nkhaniyi, tifufuza dziko losangalatsa la kutsegula magetsi ndikuwona momwe zosakaniza zimalekanitsidwira mkati mwa centrifuge.

Kumvetsetsa Mphamvu ya Centrifugal

Kuti mumvetse momwe zosakaniza zimalekanitsidwira mkati mwa centrifuge, ndikofunikira kumvetsetsa mphamvu ya centrifugal. Chinthu chikayikidwa mu centrifuge, chimazungulira mofulumira, ndikupanga mphamvu ya centrifugal yolunjika kunja kuchokera pakati. Zigawo zomwe zili mkati mwa chosakanizacho zimakumana ndi kukula kosiyana kwa mphamvu iyi kutengera kuchuluka kwawo. Tinthu tolemera timakakamizika kupita kumphepete kwakunja kwa chipinda chozungulira, chomwe chimatchedwa munda wa mphamvu ya centrifugal.

Kutaya kwa nthaka: Kulekanitsidwa ndi kuchulukana kwa nthaka

Chimodzi mwa njira zazikulu zomwe zimagwiritsidwa ntchito mu centrifuge ndi sedimentation. Mu njirayi, zigawo zokhuthala mkati mwa chisakanizo zimakhazikika pansi pa chubu cha centrifuge, ndikupanga pellet kapena sediment. Izi zimathandiza kuti zigawo zomwe zili ndi makulidwe osiyanasiyana zilekanitsidwe. Mwachitsanzo, pakulekanitsidwa kwa magazi, maselo ofiira a magazi (RBCs) amakhala okhuthala ndipo amakhala pansi, pomwe plasma, yokhala ndi zigawo zina, imakhalabe mu supernatant. Pochotsa supernatant mosamala, ma RBC amatha kugawidwa kuti akawunikidwenso kapena kuchitidwa opaleshoni.

Kuthamanga kwa Centrifugation ndi Kukonza Nthawi

Kupambana kwa centrifugation kumadalira kwambiri kudziwa liwiro loyenera komanso nthawi yopatukana. Zinthu zosiyanasiyana kapena tinthu tating'onoting'ono tingafunike kusintha makonda a centrifuge kuti tipeze kulekana koyenera. Mwa kusintha liwiro lozungulira ndi nthawi, asayansi amatha kusintha njirayi kuti igwirizane ndi zigawo kapena tinthu tating'onoting'ono tomwe timafunikira. Kuthamanga mwachangu kumapanga mphamvu zambiri za centrifugation, zomwe zimathandiza kulekanitsa tinthu tating'onoting'ono kapena mankhwala. Komabe, mphamvu zambiri za centrifugation zimatha kuwononga tinthu tating'onoting'ono kapena maselo osalimba, zomwe zimagogomezera kufunika kopeza bwino.

Njira Zogwiritsira Ntchito Centrifugation: Kusiyana ndi Kuchuluka kwa Maginito

Mu gawo la centrifugation, njira ziwiri zazikulu zimagwiritsidwa ntchito polekanitsa zosakaniza: differential centrifugation ndi density gradient centrifugation. Differential centrifugation imaphatikizapo maulendo angapo a centrifugation pa liwiro lowonjezera, zomwe zimathandiza kuti zigawo zosiyanasiyana zilekanitsidwe pazigawo zosiyanasiyana. Mwa kusintha nthawi ndi liwiro la sitepe iliyonse yotsatira, asayansi amatha pang'onopang'ono kusiyanitsa zigawo zomwe zili ndi kukula ndi kuchulukana kosiyanasiyana.

Kumbali inayi, kugawa kwa maginito pogwiritsa ntchito chubu cha centrifuge chomwe chili ndi yankho la maginito pogwiritsa ntchito density gradient. Njirayi imapanga maginito ozungulira, zomwe zimathandiza kuti tinthu tikhazikike m'malo osiyanasiyana kutengera kuchulukana kwawo. Mwa kuyika mosamala chisakanizocho pamwamba pa gradient, tinthu tomwe tili ndi maginito ofanana timasamukira kumalo enaake mkati mwa chubu cha centrifuge, zomwe zimathandiza kuti tisiyane. Kugawa kwa maginito pogwiritsa ntchito density gradient kumathandiza kwambiri pochita zinthu ndi zosakaniza zomwe zili ndi zigawo zogwirizana kwambiri kapena tinthu tomwe timasiyana pang'ono mu kuchulukana.

Mapeto:

Kugawa zinthu pogwiritsa ntchito centrifugation ndi njira yothandiza komanso yofunika kwambiri yolekanitsira zinthu zosiyanasiyana m'mafakitale osiyanasiyana asayansi, azachipatala, ndi mafakitale. Pogwiritsa ntchito mphamvu ya centrifugal ndi sedimentation, asayansi amatha kulekanitsa bwino zinthu kutengera kuchuluka kwake. Mwa kukonza bwino malo ogawa zinthu pogwiritsa ntchito centrifugation ndikugwiritsa ntchito njira monga differential and density gradient centrifugation, ofufuza amatha kusiyanitsa zinthu zinazake kapena tinthu tating'onoting'ono molondola komanso moyenera. Ndi kupita patsogolo kwa ukadaulo wa centrifuge, chida chofunikira ichi chikupitilizabe kusintha magawo osiyanasiyana ophunzirira ndikuwonjezera kumvetsetsa kwathu kwa zinthu zovuta zosakanikirana.