Pehea e hoʻokaʻawale ʻia ai nā hui ʻana i loko o kahi centrifuge
He Hoʻolauna i ka Centrifugation
He ʻano hana i hoʻohana nui ʻia ka centrifugation ma nā ʻano ʻepekema like ʻole, lapaʻau, a me nā ʻoihana no ka hoʻokaʻawale ʻana i nā hui ʻana ma muli o ko lākou mau mānoanoa. Pili kēia hana kūpono i ka hoʻohana ʻana i kahi mīkini centrifuge, kahi e hoʻopili ai i ka ikaika centrifugal e hoʻokaʻawale i nā ʻāpana o kahi hui ʻana. I nā makahiki i hala iho nei, ua lilo ka centrifugation i mea hana pono ʻole i nā keʻena noiʻi, nā kikowaena diagnostic, a me nā wahi ʻoihana ma muli o kona hiki ke unuhi i nā mea kikoʻī a me nā ʻāpana mai nā hui paʻakikī. Ma kēia ʻatikala, e luʻu mākou i loko o ke ao hoihoi o ka centrifugation a e ʻimi pehea e hoʻokaʻawale ʻia ai nā hui ʻana i loko o kahi centrifuge.
Ke Hoʻomaopopo ʻana i ka Mana Centrifugal
I mea e hoʻomaopopo ai pehea e hoʻokaʻawale ʻia ai nā hui ʻana i loko o kahi centrifuge, he mea nui e hoʻomaopopo i ka ikaika centrifugal. Ke kau ʻia kahi mea i loko o kahi centrifuge, e wili wikiwiki ia, e hana ana i kahi ikaika centrifugal i kuhikuhi ʻia i waho mai ke kikowaena. ʻIke nā ʻāpana i loko o ka hui ʻana i nā nui like ʻole o kēia ikaika ma muli o ko lākou mau mānoanoa. Hoʻoikaika ʻia nā ʻāpana kaumaha i nā ʻaoʻao o waho o ke keʻena wili, i kapa ʻia ʻo ke kahua ikaika centrifugal.
Sedimentation: Hoʻokaʻawale ʻana ma ka Density
ʻO kekahi o nā ʻano hana nui i hoʻohana ʻia i loko o kahi centrifuge ʻo ia ka sedimentation. Ma kēia kaʻina hana, ʻoi aku ka paʻa o nā ʻāpana i loko o kahi hui ʻana e noho ana ma lalo o ka ʻōmole centrifuge, e hana ana i kahi pellet a i ʻole sediment. ʻAe kēia i ka hoʻokaʻawale ʻana o nā ʻāpana me nā densities like ʻole. No ka laʻana, i ka hoʻokaʻawale ʻana o ke koko, ʻoi aku ka paʻa o nā cell koko ʻulaʻula (RBC) a noho ma lalo, ʻoiai ʻo ka plasma, nona nā ʻāpana ʻē aʻe, e noho ana i loko o ka supernatant. Ma ka wehe pono ʻana i ka supernatant, hiki ke hoʻokaʻawale ʻia nā RBC no ka nānā hou ʻana a i ʻole nā kaʻina hana lapaʻau.
Ka wikiwiki o ka centrifugation a me ka hoʻonui ʻana i ka manawa
ʻO ka holomua o ka centrifugation e hilinaʻi nui ʻia i ka hoʻoholo ʻana i ka wikiwiki kūpono a me ka lōʻihi no ka hoʻokaʻawale ʻana. Pono paha nā mea like ʻole a i ʻole nā ʻāpana i nā hoʻonohonoho centrifuge like ʻole e hoʻokō i ka hoʻokaʻawale kūpono. Ma ka hoʻoponopono ʻana i ka wikiwiki a me ka manawa rotational, hiki i nā kānaka ʻepekema ke hoʻopilikino i ke kaʻina hana e kuhikuhi pono i nā ʻāpana a i ʻole nā ʻāpana i makemake ʻia. Hoʻoulu nā wikiwiki wikiwiki i nā ikaika centrifugal kiʻekiʻe, e kōkua ana i ka hoʻokaʻawale ʻana i nā ʻāpana maikaʻi a i ʻole nā hui. Eia nō naʻe, hiki i nā ikaika centrifugal nui ke hōʻino i nā ʻāpana a i ʻole nā pūnaewele palupalu, e hoʻoikaika ana i ka pono o ka loaʻa ʻana o ke kaulike kūpono.
Nā ʻenehana Centrifugation: ʻokoʻa a me ke gradient density
I loko o ke aupuni o ka centrifugation, ʻelua mau ʻano hana mua i hoʻohana ʻia no ka hoʻokaʻawale ʻana i nā hui: ka centrifugation differential a me ka centrifugation density gradient. Pili ka centrifugation differential i nā pōʻai he nui o ka centrifugation ma ka wikiwiki e hoʻonui ana, e ʻae ana i ka hoʻokaʻawale ʻana o nā ʻāpana like ʻole ma nā pae like ʻole. Ma ka hoʻoponopono ʻana i ka lōʻihi a me ka wikiwiki no kēlā me kēia ʻanuʻu ma hope, hiki i nā kānaka ʻepekema ke hoʻokaʻawale mālie i nā ʻāpana me nā nui a me nā densities like ʻole.
Ma ka ʻaoʻao ʻē aʻe, hoʻohana ka centrifugation density gradient i kahi paipu centrifuge e loaʻa ana kahi hopena density gradient. Hoʻokumu kēia hopena i kahi gradient concentration, e ʻae ana i nā ʻāpana e noho ma nā kūlana like ʻole ma muli o ko lākou mau densities. Ma ka hoʻopili pono ʻana i ka hui ʻana ma luna o ka gradient, neʻe nā ʻāpana me nā densities like i nā kūlana kikoʻī i loko o ka paipu centrifuge, e hoʻomaʻalahi ana i ko lākou kaʻawale ʻana. He mea pono loa ka centrifugation density gradient i ka wā e hana ai me nā hui ʻana e loaʻa ana nā ʻāpana pili kokoke a i ʻole nā ʻāpana e ʻokoʻa iki wale nō i ka density.
Hopena:
He ʻano hana maʻalahi a koʻikoʻi ka centrifugation no ka hoʻokaʻawale ʻana i nā hui ʻana ma nā ʻano noiʻi ʻepekema, lapaʻau, a me nā ʻoihana like ʻole. Ma o ka hoʻohana ʻana i ka ikaika centrifugal a me ka sedimentation, hiki i nā kānaka ʻepekema ke hoʻokaʻawale pololei i nā ʻāpana ma muli o ko lākou mau densities. Ma ka hoʻomaikaʻi ʻana i nā hoʻonohonoho centrifuge a me ka hoʻohana ʻana i nā ʻano hana e like me ka differential and density gradient centrifugation, hiki i nā mea noiʻi ke hoʻokaʻawale i nā mea kikoʻī a i ʻole nā ʻāpana me ka pololei a me ka pono. Me ka holomua mau ʻana i ka ʻenehana centrifuge, ke hoʻomau nei kēia mea hana pono e hoʻololi i nā ʻano like ʻole o ke aʻo ʻana a hoʻonui i ko mākou ʻike i nā hui paʻakikī.
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